##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BamHI BbrPI BciVI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
BspDI BspEI BsrBI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I FauI FseI FspI
HindIII KpnI KspI MluI MroI
NheI NotI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AclI 303 AACGTT AA^CGTT
AgeI 2140 ACCGGT A^CCGGT
Alw44I 2448 GTGCAC G^TGCAC
AlwNI 3781 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2012 CCTGAGG CC^T[ACGT]AGG
ApaLI 2448 GTGCAC G^TGCAC
AseI 3551 ATTAAT AT^TAAT
AsnI 3551 ATTAAT AT^TAAT
Asp700I 2884 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 23 GAGCTC G[GA]GC[CT]^C
BbsI 3495 GAAGAC GAAGAC(2/6)
BcgI 88 CGAGCTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 75 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1221 AGATCT A^GATCT
BpuAI 3495 GAAGAC GAAGAC(2/6)
BsaAI 2152 TACGTG [CT]AC^GT[GA]
BsaBI 1974 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2140 ACCGGT [AT]^CCGG[AT]
BsmFI 3108 GTCCC GGGAC(10/14)
Bsp1407I 828 TGTACA T^GTACA
BsrGI 828 TGTACA T^GTACA
BssHII 3097 GCGCGC G^CGCGC
Bsu36I 2012 CCTGAGG CC^T[ACGT]AGG
DraII 2412 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 209 GAAGAG CTCTTC(1/4)
Eco81I 2012 CCTGAGG CC^T[ACGT]AGG
EcoO109I 2412 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1007 GAATTC G^AATTC
HgaI 12 GCGTC GACGC(5/10)
HincII 2709 GTTAAC GT[CT]^[GA]AC
HindII 2709 GTTAAC GT[CT]^[GA]AC
HpaI 2709 GTTAAC GTT^AAC
Ksp632I 209 GAAGAG CTCTTC(1/4)
MamI 1974 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 999 CAATTG C^AATTG
MunI 999 CAATTG C^AATTG
NaeI 620 GCCGGC GCC^GGC
NgoMIV 620 GCCGGC G^CCGGC
NruI 114 TCGCGA TCG^CGA
PinAI 2140 ACCGGT A^CCGGT
PpuMI 2412 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3551 ATTAAT AT^TAAT
Psp1406I 303 AACGTT AA^CGTT
SacI 23 GAGCTC GAGCT^C
SpeI 2886 ACTAGT A^CTAGT
SspBI 828 TGTACA T^GTACA
XbaI 1079 TCTAGA T^CTAGA
XmnI 2884 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 890 GTCTAC GT^[AC][GT]AC
3568 GTAGAC
AcyI 83 GGCGCC G[GA]^CG[CT]C
1965 GGCGCC
AflII 1727 CTTAAG C^TTAAG
2791 CTTAAG
AvaII 2413 GGACC G^G[AT]CC
3107 GGTCC
BanI 83 GGCGCC G^G[CT][GA]CC
1965 GGCGCC
BbeI 83 GGCGCC GGCGC^C
1965 GGCGCC
BcnI 1327 CCCGG CC^[GC]GG
3834 CCGGG
BfrI 1727 CTTAAG C^TTAAG
2791 CTTAAG
BsaHI 83 GGCGCC G[GA]^CG[CT]C
1965 GGCGCC
BsmBI 28 CGTCTC CGTCTC(1/5)
1866 CGTCTC
BspLU11I 462 ACATGT A^CATGT
2991 ACATGT
BspMI 159 ACCTGC ACCTGC(4/8)
3977 GCAGGT
BsrFI 620 GCCGGC [GA]^CCGG[CT]
2140 ACCGGT
BssSI 1852 CACGAG CACGAG(-5/-1)
3678 CACGAG
BstAPI 1391 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1436 GCACTCCTTGC
Cfr10I 620 GCCGGC [GA]^CCGG[CT]
2140 ACCGGT
Hin1I 83 GGCGCC G[GA]^CG[CT]C
1965 GGCGCC
KasI 83 GGCGCC G^GCGCC
1965 GGCGCC
NarI 83 GGCGCC GG^CGCC
1965 GGCGCC
NciI 1327 CCCGG CC^[GC]GG
3834 CCGGG
PshAI 317 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2796 GACAGTGGTC
PstI 788 CTGCAG CTGCA^G
3165 CTGCAG
PvuII 64 CAGCTG CAG^CTG
3112 CAGCTG
SfoI 83 GGCGCC GGC^GCC
1965 GGCGCC
SspI 971 AATATT AAT^ATT
1017 AATATT
StuI 339 AGGCCT AGG^CCT
3018 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AspI
AvaI BalI BbvCI BbvI BclI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI BsoBI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PflFI PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII