################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI BamHI BciVI BclI BfrI BglII BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraIII DrdI Eam1105I EcoRI EcoRV FbaI HincII HindII HindIII HpaI KpnI MamI MfeI MluI MunI NheI NotI NspV PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SfiI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AatII 955 GACGTC GACGT^C AccIII 1033 TCCGGA T^CCGGA Alw44I 3961 GTGCAC G^TGCAC ApaLI 3961 GTGCAC G^TGCAC AspI 1397 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3155 CCTAGG C^CTAGG BbvCI 1373 GCTGAGG CCTCAGC(-5/-2) BcgI 1573 GCAGGAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 3155 CCTAGG C^CTAGG BseAI 1033 TCCGGA T^CCGGA BspEI 1033 TCCGGA T^CCGGA BspHI 2667 TCATGA T^CATGA BsrDI 371 GCAATG GCAATG(2/0) BstEII 2500 GGTCACC G^GT[ACGT]ACC BstPI 2500 GGTCACC G^GT[ACGT]ACC DraI 856 TTTAAA TTT^AAA EcoO65I 2500 GGTCACC G^GT[ACGT]ACC EcoT22I 1040 ATGCAT ATGCA^T FseI 267 GGCCGGCC GGCCGG^CC MroI 1033 TCCGGA T^CCGGA NdeI 574 CATATG CA^TATG NruI 1582 TCGCGA TCG^CGA NsiI 1040 ATGCAT ATGCA^T NspI 1791 ACATGC [GA]CATG^[CT] PflFI 1397 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2494 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2667 TCATGA T^CATGA SacI 3331 GAGCTC GAGCT^C SbfI 2317 CCTGCAGG CCTGCA^GG SexAI 1416 ACCTGGT A^CC[AT]GGT Sse8387I 2317 CCTGCAGG CCTGCA^GG Tth111I 1397 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 3658 CCATTTAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 501 GAATTT [GA]^AATT[CT] 3991 AAATTT AflIII 1359 ACGTGT A^C[GA][CT]GT 2766 ACGTGT Aor51HI 1026 AGCGCT AGC^GCT 2328 AGCGCT ApoI 501 GAATTT [GA]^AATT[CT] 3991 AAATTT AviII 1248 TGCGCA TGC^GCA 2682 TGCGCA BbrPI 2765 CACGTG CAC^GTG 3751 CACGTG BlpI 2109 GCTCAGC GC^T[ACGT]AGC 3333 GCTCAGC Bpu1102I 2109 GCTCAGC GC^T[ACGT]AGC 3333 GCTCAGC BsaAI 2765 CACGTG [CT]AC^GT[GA] 3751 CACGTG BsaWI 1033 TCCGGA [AT]^CCGG[AT] 1492 ACCGGA BsmI 2560 GAATGC GAATGC(1/-1) 2975 GAATGC CelII 2109 GCTCAGC GC^T[ACGT]AGC 3333 GCTCAGC Eco47III 1026 AGCGCT AGC^GCT 2328 AGCGCT FspI 1248 TGCGCA TGC^GCA 2682 TGCGCA PmaCI 2765 CACGTG CAC^GTG 3751 CACGTG PmlI 2765 CACGTG CAC^GTG 3751 CACGTG SapI 2216 GAAGAGC GCTCTTC(1/4) 1311 GCTCTTC SgrAI 805 CGCCGGCG C[GA]^CCGG[CT]G 1727 CGCCGGCG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI