##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspI        AvaI        AviII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BfrI        
BglI        BglII       BlpI        Bpu1102I    BsaAI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstBI       BstUI       BstXI       
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       EcoT22I     FauI        
FseI        FspI        HgaI        Hin1I       HpaI        
KasI        KpnI        KspI        MluI        MroI        
MspA1I      MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AflIII       1812  ACATGT               A^C[GA][CT]GT
AseI         2830  ATTAAT               AT^TAAT
AsnI         2830  ATTAAT               AT^TAAT
BalI         3310  TGGCCA               TGG^CCA
BanI          910  GGTGCC               G^G[CT][GA]CC
BciVI        2872  GTATCC               GTATCC(6/5)
BcnI         1942  CCCGG                CC^[GC]GG
BsaBI         921  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2298  ACCGGA               [AT]^CCGG[AT]
BsgI         3199  GTGCAG               GTGCAG(16/14)
BspLU11I     1812  ACATGT               A^CATGT
BstAPI         27  GCAGTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        527  GGTGACC              G^GT[ACGT]ACC
BstPI         527  GGTGACC              G^GT[ACGT]ACC
BstYI         569  AGATCC               [GA]^GATC[CT]
CfoI         1510  GCGC                 GCG^C
DsaI          840  CCATGG               C^C[GA][CT]GG
EaeI         3310  TGGCCA               [CT]^GGCC[GA]
EcoO65I       527  GGTGACC              G^GT[ACGT]ACC
HaeII        1509  AGCGCC               [GA]GCGC^[CT]
HhaI         1510  GCGC                 GCG^C
HinP1I       1510  GCGC                 G^CGC
HincII        370  GTCAAC               GT[CT]^[GA]AC
HindII        370  GTCAAC               GT[CT]^[GA]AC
MamI          921  GATGATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          569  AGATCC               [GA]^GATC[CT]
MluNI        3310  TGGCCA               TGG^CCA
MscI         3310  TGGCCA               TGG^CCA
NciI         1942  CCCGG                CC^[GC]GG
NcoI          840  CCATGG               C^CATGG
PacI         1107  TTAATTAA             TTAAT^TAA
PshAI        2708  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2830  ATTAAT               AT^TAAT
SacI          381  GAGCTC               GAGCT^C
SspI          955  AATATT               AAT^ATT
StuI         2354  AGGCCT               AGG^CCT
XcmI          950  CCAGCAATATTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         569  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AocI          764  CCTCAGG              CC^T[ACGT]AGG
              914  CCTCAGG             
AvrII        3143  CCTAGG               C^CTAGG
             3375  CCTAGG              
BanII         381  GAGCTC               G[GA]GC[CT]^C
             3071  GGGCTC              
BbsI         2333  GAAGAC               GAAGAC(2/6)
             3416  GAAGAC              
BclI          926  TGATCA               T^GATCA
             2473  TGATCA              
BlnI         3143  CCTAGG               C^CTAGG
             3375  CCTAGG              
BpuAI        2333  GAAGAC               GAAGAC(2/6)
             3416  GAAGAC              
BsaI          401  GAGACC               GGTCTC(1/5)
             1745  GAGACC              
BsmI         2604  GAATGC               GAATGC(1/-1)
             3172  GAATGC              
Bsu36I        764  CCTCAGG              CC^T[ACGT]AGG
              914  CCTCAGG             
Cac8I         161  GCAAGC               GC[ACGT]^[ACGT]GC
             3337  GCTTGC              
DraI         2340  TTTAAA               TTT^AAA
             3462  TTTAAA              
DraII         238  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1320  AGGTCCC             
Eco81I        764  CCTCAGG              CC^T[ACGT]AGG
              914  CCTCAGG             
EcoO109I      238  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1320  AGGTCCC             
FbaI          926  TGATCA               T^GATCA
             2473  TGATCA              
HindIII      1579  AAGCTT               A^AGCTT
             3645  AAGCTT              
MaeII        1295  ACGT                 A^CGT
             2016  ACGT                
MfeI         1820  CAATTG               C^AATTG
             3500  CAATTG              
MunI         1820  CAATTG               C^AATTG
             3500  CAATTG              
NspI         1812  ACATGT               [GA]CATG^[CT]
             3569  GCATGT              
PpuMI         238  AGGTCCC              [GA]G^G[AT]CC[CT]
             1320  AGGTCCC             
TaqI          222  TCGA                 T^CGA
             2261  TCGA                
TthHB8I       222  TCGA                 T^CGA
             2261  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
Bst1107I    BstF5I      BstNI       BstZ17I     Cfr13I      
DdeI        DpnII       EarI        EcoRI       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XmnI