################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstUI BstXI CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FauI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MluI MroI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AflIII 1812 ACATGT A^C[GA][CT]GT AseI 2830 ATTAAT AT^TAAT AsnI 2830 ATTAAT AT^TAAT BalI 3310 TGGCCA TGG^CCA BanI 910 GGTGCC G^G[CT][GA]CC BciVI 2872 GTATCC GTATCC(6/5) BcnI 1942 CCCGG CC^[GC]GG BsaBI 921 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2298 ACCGGA [AT]^CCGG[AT] BsgI 3199 GTGCAG GTGCAG(16/14) BspLU11I 1812 ACATGT A^CATGT BstAPI 27 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 527 GGTGACC G^GT[ACGT]ACC BstPI 527 GGTGACC G^GT[ACGT]ACC BstYI 569 AGATCC [GA]^GATC[CT] CfoI 1510 GCGC GCG^C DsaI 840 CCATGG C^C[GA][CT]GG EaeI 3310 TGGCCA [CT]^GGCC[GA] EcoO65I 527 GGTGACC G^GT[ACGT]ACC HaeII 1509 AGCGCC [GA]GCGC^[CT] HhaI 1510 GCGC GCG^C HinP1I 1510 GCGC G^CGC HincII 370 GTCAAC GT[CT]^[GA]AC HindII 370 GTCAAC GT[CT]^[GA]AC MamI 921 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 569 AGATCC [GA]^GATC[CT] MluNI 3310 TGGCCA TGG^CCA MscI 3310 TGGCCA TGG^CCA NciI 1942 CCCGG CC^[GC]GG NcoI 840 CCATGG C^CATGG PacI 1107 TTAATTAA TTAAT^TAA PshAI 2708 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2830 ATTAAT AT^TAAT SacI 381 GAGCTC GAGCT^C SspI 955 AATATT AAT^ATT StuI 2354 AGGCCT AGG^CCT XcmI 950 CCAGCAATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 569 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AocI 764 CCTCAGG CC^T[ACGT]AGG 914 CCTCAGG AvrII 3143 CCTAGG C^CTAGG 3375 CCTAGG BanII 381 GAGCTC G[GA]GC[CT]^C 3071 GGGCTC BbsI 2333 GAAGAC GAAGAC(2/6) 3416 GAAGAC BclI 926 TGATCA T^GATCA 2473 TGATCA BlnI 3143 CCTAGG C^CTAGG 3375 CCTAGG BpuAI 2333 GAAGAC GAAGAC(2/6) 3416 GAAGAC BsaI 401 GAGACC GGTCTC(1/5) 1745 GAGACC BsmI 2604 GAATGC GAATGC(1/-1) 3172 GAATGC Bsu36I 764 CCTCAGG CC^T[ACGT]AGG 914 CCTCAGG Cac8I 161 GCAAGC GC[ACGT]^[ACGT]GC 3337 GCTTGC DraI 2340 TTTAAA TTT^AAA 3462 TTTAAA DraII 238 AGGTCCC [GA]G^G[ACGT]CC[CT] 1320 AGGTCCC Eco81I 764 CCTCAGG CC^T[ACGT]AGG 914 CCTCAGG EcoO109I 238 AGGTCCC [GA]G^G[ACGT]CC[CT] 1320 AGGTCCC FbaI 926 TGATCA T^GATCA 2473 TGATCA HindIII 1579 AAGCTT A^AGCTT 3645 AAGCTT MaeII 1295 ACGT A^CGT 2016 ACGT MfeI 1820 CAATTG C^AATTG 3500 CAATTG MunI 1820 CAATTG C^AATTG 3500 CAATTG NspI 1812 ACATGT [GA]CATG^[CT] 3569 GCATGT PpuMI 238 AGGTCCC [GA]G^G[AT]CC[CT] 1320 AGGTCCC TaqI 222 TCGA T^CGA 2261 TCGA TthHB8I 222 TCGA T^CGA 2261 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI Bst1107I BstF5I BstNI BstZ17I Cfr13I DdeI DpnII EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI