##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AccIII      AclI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AspEI       AspI        
AviII       BbeI        BbrPI       BclI        BseAI       
BsiWI       BspDI       BspEI       BspLU11I    BsrBI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     FbaI        FseI        FspI        HaeII       
HgaI        KasI        KspI        MfeI        MluI        
MroI        MunI        NarI        NotI        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SbfI        SfoI        Sse8387I    Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflIII       5922  ACACGT               A^C[GA][CT]GT
AgeI         5795  ACCGGT               A^CCGGT
AvrII        6231  CCTAGG               C^CTAGG
BamHI        3137  GGATCC               G^GATCC
BciVI        1117  GTATCC               GTATCC(6/5)
BlnI         6231  CCTAGG               C^CTAGG
BsaBI        1852  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        5885  CGGCCG               CG[GA][CT]^CG
BsmBI        6136  GAGACG               CGTCTC(1/5)
Bsp1407I     2303  TGTACA               T^GTACA
BsrGI        2303  TGTACA               T^GTACA
BstBI        1010  TTCGAA               TT^CGAA
DsaI          266  CCATGG               C^C[GA][CT]GG
EagI         5885  CGGCCG               C^GGCCG
EclXI        5885  CGGCCG               C^GGCCG
Eco52I       5885  CGGCCG               C^GGCCG
FauI         4394  GCGGG                CCCGC(4/6)
MamI         1852  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         3823  GCCGGC               GCC^GGC
NcoI          266  CCATGG               C^CATGG
NgoMIV       3823  GCCGGC               G^CCGGC
NheI         1555  GCTAGC               G^CTAGC
NruI          175  TCGCGA               TCG^CGA
NspV         1010  TTCGAA               TT^CGAA
PflMI        4346  CCATGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        5795  ACCGGT               A^CCGGT
PpuMI         975  GGGACCT              [GA]G^G[AT]CC[CT]
SalI          852  GTCGAC               G^TCGAC
SexAI        1325  ACCAGGT              A^CC[AT]GGT
SfuI         1010  TTCGAA               TT^CGAA
SgrAI        5794  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         1291  CCCGGG               CCC^GGG
SnaBI        6496  TACGTA               TAC^GTA
SpeI         2217  ACTAGT               A^CTAGT
SphI         3092  GCATGC               GCATG^C
SspBI        2303  TGTACA               T^GTACA
Van91I       4346  CCATGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1291  CCCGGG               C^CCGGG
XmaI         1291  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AatII        1466  GACGTC               GACGT^C
             6138  GACGTC              
Acc65I       1322  GGTACC               G^GTACC
             5230  GGTACC              
AccI          526  GTAGAC               GT^[AC][GT]AC
              852  GTCGAC              
AcyI         1466  GACGTC               G[GA]^CG[CT]C
             6138  GACGTC              
AflII        3154  CTTAAG               C^TTAAG
             5091  CTTAAG              
AseI         4903  ATTAAT               AT^TAAT
             6787  ATTAAT              
AsnI         4903  ATTAAT               AT^TAAT
             6787  ATTAAT              
Asp718I      1322  GGTACC               G^GTACC
             5230  GGTACC              
AvaI          240  CCCGAG               C^[CT]CG[GA]G
             1291  CCCGGG              
BalI         1745  TGGCCA               TGG^CCA
             5855  TGGCCA              
BcgI         3395  GCATTAACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5626  GCAACTTCCTCG        
BfrI         3154  CTTAAG               C^TTAAG
             5091  CTTAAG              
BglII        2644  AGATCT               A^GATCT
             4475  AGATCT              
BlpI          193  GCTCAGC              GC^T[ACGT]AGC
             3753  GCTGAGC             
Bpu1102I      193  GCTCAGC              GC^T[ACGT]AGC
             3753  GCTGAGC             
BsaHI        1466  GACGTC               G[GA]^CG[CT]C
             6138  GACGTC              
BsaWI          20  ACCGGA               [AT]^CCGG[AT]
             5795  ACCGGT              
BsoBI         240  CCCGAG               C^[CT]CG[GA]G
             1291  CCCGGG              
BspHI        3002  TCATGA               T^CATGA
             5467  TCATGA              
BsrFI        3823  GCCGGC               [GA]^CCGG[CT]
             5795  ACCGGT              
BstAPI       3092  GCATGCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6658  GCATTTATTGC         
BstXI        3580  CCATTCAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5395  CCACAGGGGTGG        
CelII         193  GCTCAGC              GC^T[ACGT]AGC
             3753  GCTGAGC             
Cfr10I       3823  GCCGGC               [GA]^CCGG[CT]
             5795  ACCGGT              
Hin1I        1466  GACGTC               G[GA]^CG[CT]C
             6138  GACGTC              
HindIII      3151  AAGCTT               A^AGCTT
             5081  AAGCTT              
HpaI         6677  GTTAAC               GTT^AAC
             6692  GTTAAC              
KpnI         1322  GGTACC               GGTAC^C
             5230  GGTACC              
MluNI        1745  TGGCCA               TGG^CCA
             5855  TGGCCA              
MscI         1745  TGGCCA               TGG^CCA
             5855  TGGCCA              
NdeI         1775  CATATG               CA^TATG
             3849  CATATG              
PshBI        4903  ATTAAT               AT^TAAT
             6787  ATTAAT              
RcaI         3002  TCATGA               T^CATGA
             5467  TCATGA              
SfiI         1341  GGCCTTCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             4846  GGCCAATGAGGCC       
XcmI         1194  CCACCCCCGTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             6333  CCACTTTGGTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        Asp700I     
AspHI       AvaII       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        EcoO109I    EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI