################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccIII AclI AhdI Alw44I AocI Aor51HI ApaLI AscI AspEI AspI AviII BbeI BbrPI BclI BseAI BsiWI BspDI BspEI BspLU11I BsrBI BssHII BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I FbaI FseI FspI HaeII HgaI KasI KspI MfeI MluI MroI MunI NarI NotI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI SfoI Sse8387I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AflIII 5922 ACACGT A^C[GA][CT]GT AgeI 5795 ACCGGT A^CCGGT AvrII 6231 CCTAGG C^CTAGG BamHI 3137 GGATCC G^GATCC BciVI 1117 GTATCC GTATCC(6/5) BlnI 6231 CCTAGG C^CTAGG BsaBI 1852 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 5885 CGGCCG CG[GA][CT]^CG BsmBI 6136 GAGACG CGTCTC(1/5) Bsp1407I 2303 TGTACA T^GTACA BsrGI 2303 TGTACA T^GTACA BstBI 1010 TTCGAA TT^CGAA DsaI 266 CCATGG C^C[GA][CT]GG EagI 5885 CGGCCG C^GGCCG EclXI 5885 CGGCCG C^GGCCG Eco52I 5885 CGGCCG C^GGCCG FauI 4394 GCGGG CCCGC(4/6) MamI 1852 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 3823 GCCGGC GCC^GGC NcoI 266 CCATGG C^CATGG NgoMIV 3823 GCCGGC G^CCGGC NheI 1555 GCTAGC G^CTAGC NruI 175 TCGCGA TCG^CGA NspV 1010 TTCGAA TT^CGAA PflMI 4346 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 5795 ACCGGT A^CCGGT PpuMI 975 GGGACCT [GA]G^G[AT]CC[CT] SalI 852 GTCGAC G^TCGAC SexAI 1325 ACCAGGT A^CC[AT]GGT SfuI 1010 TTCGAA TT^CGAA SgrAI 5794 CACCGGTG C[GA]^CCGG[CT]G SmaI 1291 CCCGGG CCC^GGG SnaBI 6496 TACGTA TAC^GTA SpeI 2217 ACTAGT A^CTAGT SphI 3092 GCATGC GCATG^C SspBI 2303 TGTACA T^GTACA Van91I 4346 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1291 CCCGGG C^CCGGG XmaI 1291 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 1466 GACGTC GACGT^C 6138 GACGTC Acc65I 1322 GGTACC G^GTACC 5230 GGTACC AccI 526 GTAGAC GT^[AC][GT]AC 852 GTCGAC AcyI 1466 GACGTC G[GA]^CG[CT]C 6138 GACGTC AflII 3154 CTTAAG C^TTAAG 5091 CTTAAG AseI 4903 ATTAAT AT^TAAT 6787 ATTAAT AsnI 4903 ATTAAT AT^TAAT 6787 ATTAAT Asp718I 1322 GGTACC G^GTACC 5230 GGTACC AvaI 240 CCCGAG C^[CT]CG[GA]G 1291 CCCGGG BalI 1745 TGGCCA TGG^CCA 5855 TGGCCA BcgI 3395 GCATTAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5626 GCAACTTCCTCG BfrI 3154 CTTAAG C^TTAAG 5091 CTTAAG BglII 2644 AGATCT A^GATCT 4475 AGATCT BlpI 193 GCTCAGC GC^T[ACGT]AGC 3753 GCTGAGC Bpu1102I 193 GCTCAGC GC^T[ACGT]AGC 3753 GCTGAGC BsaHI 1466 GACGTC G[GA]^CG[CT]C 6138 GACGTC BsaWI 20 ACCGGA [AT]^CCGG[AT] 5795 ACCGGT BsoBI 240 CCCGAG C^[CT]CG[GA]G 1291 CCCGGG BspHI 3002 TCATGA T^CATGA 5467 TCATGA BsrFI 3823 GCCGGC [GA]^CCGG[CT] 5795 ACCGGT BstAPI 3092 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6658 GCATTTATTGC BstXI 3580 CCATTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5395 CCACAGGGGTGG CelII 193 GCTCAGC GC^T[ACGT]AGC 3753 GCTGAGC Cfr10I 3823 GCCGGC [GA]^CCGG[CT] 5795 ACCGGT Hin1I 1466 GACGTC G[GA]^CG[CT]C 6138 GACGTC HindIII 3151 AAGCTT A^AGCTT 5081 AAGCTT HpaI 6677 GTTAAC GTT^AAC 6692 GTTAAC KpnI 1322 GGTACC GGTAC^C 5230 GGTACC MluNI 1745 TGGCCA TGG^CCA 5855 TGGCCA MscI 1745 TGGCCA TGG^CCA 5855 TGGCCA NdeI 1775 CATATG CA^TATG 3849 CATATG PshBI 4903 ATTAAT AT^TAAT 6787 ATTAAT RcaI 3002 TCATGA T^CATGA 5467 TCATGA SfiI 1341 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4846 GGCCAATGAGGCC XcmI 1194 CCACCCCCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 6333 CCACTTTGGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoRV EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StuI StyI SwaI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI