##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AspEI AviII BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BglI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI FauI FbaI
FseI FspI HapII Hin1I HpaII
KasI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
StuI SwaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2605 GGTACC G^GTACC
AccI 7056 GTCTAC GT^[AC][GT]AC
Aor51HI 543 AGCGCT AGC^GCT
ApaI 6954 GGGCCC GGGCC^C
Asp718I 2605 GGTACC G^GTACC
BalI 6125 TGGCCA TGG^CCA
BglII 7526 AGATCT A^GATCT
BsaAI 6567 TACGTG [CT]AC^GT[GA]
BsmI 7000 GAATGC GAATGC(1/-1)
BstBI 3318 TTCGAA TT^CGAA
BstYI 7526 AGATCT [GA]^GATC[CT]
CfoI 544 GCGC GCG^C
DraIII 1430 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1260 CCATGG C^C[GA][CT]GG
EaeI 6125 TGGCCA [CT]^GGCC[GA]
Eco47III 543 AGCGCT AGC^GCT
EcoRV 2430 GATATC GAT^ATC
HaeII 543 AGCGCT [GA]GCGC^[CT]
HgaI 4891 GACGC GACGC(5/10)
HhaI 544 GCGC GCG^C
HinP1I 544 GCGC G^CGC
KpnI 2605 GGTACC GGTAC^C
MflI 7526 AGATCT [GA]^GATC[CT]
MluNI 6125 TGGCCA TGG^CCA
MscI 6125 TGGCCA TGG^CCA
NcoI 1260 CCATGG C^CATGG
NdeI 6923 CATATG CA^TATG
NspV 3318 TTCGAA TT^CGAA
PstI 1754 CTGCAG CTGCA^G
PvuII 1188 CAGCTG CAG^CTG
SfuI 3318 TTCGAA TT^CGAA
SpeI 2883 ACTAGT A^CTAGT
SphI 6752 GCATGC GCATG^C
XhoII 7526 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 3803 CCGC CCGC(-3/-1)
4812 GCGG
AspI 3498 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5953 GACTGTGTC
AvrII 4780 CCTAGG C^CTAGG
4852 CCTAGG
BlnI 4780 CCTAGG C^CTAGG
4852 CCTAGG
BsgI 5465 CTGCAC GTGCAG(16/14)
7047 GTGCAG
BspHI 2072 TCATGA T^CATGA
6467 TCATGA
BssSI 3289 CACGAG CACGAG(-5/-1)
3830 CTCGTG
HindIII 316 AAGCTT A^AGCTT
7690 AAGCTT
MspA1I 1188 CAGCTG C[AC]G^C[GT]G
3803 CCGCTG
PflFI 3498 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5953 GACTGTGTC
PflMI 2289 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3826 CCAGCTCGTGG
PpuMI 2091 AGGACCT [GA]G^G[AT]CC[CT]
6943 AGGTCCC
RcaI 2072 TCATGA T^CATGA
6467 TCATGA
SacI 2898 GAGCTC GAGCT^C
5689 GAGCTC
ScaI 4207 AGTACT AGT^ACT
8004 AGTACT
SfcI 1754 CTGCAG C^T[GA][CT]AG
5831 CTACAG
Tth111I 3498 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5953 GACTGTGTC
Van91I 2289 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3826 CCAGCTCGTGG
XbaI 4396 TCTAGA T^CTAGA
4645 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AcsI AfaI AflII AflIII AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BfaI BfrI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI Eco81I EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HincII HindII HinfI HpaI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MslI MunI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI