##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AocI ApaI AscI
Asp718I AviII AvrII BalI BbeI
BbrPI BcgI BfrI BlnI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
BstBI BstUI Bsu36I ClaI CpoI
DrdI DsaI EaeI EagI EclXI
Eco52I Eco81I FseI FspI HgaI
Hin1I KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SnaBI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 3249 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3957 AGCGCT AGC^GCT
AspEI 3249 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2826 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1441 CCCGGG C^[CT]CG[GA]G
BbvCI 4169 GCTGAGG CCTCAGC(-5/-2)
BglI 3618 GCCTCTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 6435 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1441 CCCGGG C^[CT]CG[GA]G
BspHI 3650 TCATGA T^CATGA
Bst1107I 1011 GTATAC GTA^TAC
BstEII 2154 GGTCACC G^GT[ACGT]ACC
BstPI 2154 GGTCACC G^GT[ACGT]ACC
BstZ17I 1011 GTATAC GTA^TAC
DraII 2439 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 3249 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3957 AGCGCT AGC^GCT
EcoO109I 2439 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 2154 GGTCACC G^GT[ACGT]ACC
EcoRI 6450 GAATTC G^AATTC
EcoRV 1461 GATATC GAT^ATC
MamI 6435 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PacI 6207 TTAATTAA TTAAT^TAA
PflFI 2826 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 135 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2439 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 6314 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3650 TCATGA T^CATGA
SacI 1656 GAGCTC GAGCT^C
SbfI 3725 CCTGCAGG CCTGCA^GG
ScaI 4545 AGTACT AGT^ACT
SmaI 1441 CCCGGG CCC^GGG
SphI 3218 GCATGC GCATG^C
Sse8387I 3725 CCTGCAGG CCTGCA^GG
Tth111I 2826 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 135 CCACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1441 CCCGGG C^CCGGG
XmaI 1441 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AgeI 455 ACCGGT A^CCGGT
934 ACCGGT
Alw44I 2043 GTGCAC G^TGCAC
6954 GTGCAC
ApaLI 2043 GTGCAC G^TGCAC
6954 GTGCAC
Asp700I 3246 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5611 GAATTGTTTC
BanI 458 GGTGCC G^G[CT][GA]CC
3156 GGTGCC
BanII 1656 GAGCTC G[GA]GC[CT]^C
4970 GGGCTC
BclI 878 TGATCA T^GATCA
5800 TGATCA
BcnI 1441 CCCGG CC^[GC]GG
1442 CCGGG
BlpI 3099 GCTGAGC GC^T[ACGT]AGC
3110 GCTGAGC
BpmI 1036 CTGGAG CTGGAG(16/14)
3407 CTGGAG
Bpu1102I 3099 GCTGAGC GC^T[ACGT]AGC
3110 GCTGAGC
BsmFI 5262 GGGAC GGGAC(10/14)
3024 GTCCC
BsmI 5735 GAATGC GAATGC(1/-1)
216 GCATTC
BsrDI 3614 CATTGC GCAATG(2/0)
893 GCAATG
BsrFI 455 ACCGGT [GA]^CCGG[CT]
934 ACCGGT
BstXI 2678 CCACAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4840 CCAAGAATATGG
CelII 3099 GCTGAGC GC^T[ACGT]AGC
3110 GCTGAGC
CfoI 3958 GCGC GCG^C
5349 GCGC
Cfr10I 455 ACCGGT [GA]^CCGG[CT]
934 ACCGGT
FauI 4907 CCCGC CCCGC(4/6)
1893 GCGGG
FbaI 878 TGATCA T^GATCA
5800 TGATCA
HaeII 3957 AGCGCT [GA]GCGC^[CT]
5348 AGCGCC
HhaI 3958 GCGC GCG^C
5349 GCGC
HinP1I 3958 GCGC G^CGC
5349 GCGC
HpaI 1097 GTTAAC GTT^AAC
1472 GTTAAC
MfeI 2903 CAATTG C^AATTG
7787 CAATTG
MunI 2903 CAATTG C^AATTG
7787 CAATTG
NciI 1441 CCCGG CC^[GC]GG
1442 CCGGG
PinAI 455 ACCGGT A^CCGGT
934 ACCGGT
SapI 69 GAAGAGC GCTCTTC(1/4)
3495 GAAGAGC
SpeI 4238 ACTAGT A^CTAGT
4986 ACTAGT
StuI 418 AGGCCT AGG^CCT
1282 AGGCCT
SwaI 5470 ATTTAAAT ATTT^AAAT
7000 ATTTAAAT
XcmI 2746 CCAAAAAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2900 CCACAATTGCACTGG
XmnI 3246 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5611 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BamHI BbsI
BbvI BciVI BfaI BglII BmyI
BpuAI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BspLU11I BspMI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraIII EarI EcoNI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII