################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AatII Acc65I AclI AgeI AscI AseI AsnI Asp718I BglII BsiWI BspDI BspHI Bst1107I BstBI BstZ17I ClaI EcoRI EcoRV EcoT22I FseI HpaI KpnI MfeI MluI MunI NaeI NgoMIV NotI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI SalI SfuI SgrAI SnaBI SpeI SwaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 732 AAATTT [GA]^AATT[CT] AflIII 617 ACATGT A^C[GA][CT]GT AhdI 7914 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1107 GTGCAC G^TGCAC AocI 4553 CCTCAGG CC^T[ACGT]AGG ApaLI 1107 GTGCAC G^TGCAC ApoI 732 AAATTT [GA]^AATT[CT] AspEI 7914 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 7159 CCTAGG C^CTAGG BbrPI 3219 CACGTG CAC^GTG BciVI 2206 GTATCC GTATCC(6/5) BclI 6045 TGATCA T^GATCA BlnI 7159 CCTAGG C^CTAGG BlpI 1712 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1712 GCTCAGC GC^T[ACGT]AGC BsaAI 3219 CACGTG [CT]AC^GT[GA] BsiEI 295 CGGCCG CG[GA][CT]^CG BspLU11I 617 ACATGT A^CATGT BssHII 131 GCGCGC G^CGCGC BstEII 5730 GGTGACC G^GT[ACGT]ACC BstPI 5730 GGTGACC G^GT[ACGT]ACC Bsu36I 4553 CCTCAGG CC^T[ACGT]AGG CelII 1712 GCTCAGC GC^T[ACGT]AGC CpoI 290 CGGTCCG CG^G[AT]CCG EagI 295 CGGCCG C^GGCCG Eam1105I 7914 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 295 CGGCCG C^GGCCG Eco52I 295 CGGCCG C^GGCCG Eco81I 4553 CCTCAGG CC^T[ACGT]AGG EcoO65I 5730 GGTGACC G^GT[ACGT]ACC FbaI 6045 TGATCA T^GATCA HindIII 704 AAGCTT A^AGCTT KspI 141 CCGCGG CCGC^GG NdeI 859 CATATG CA^TATG NheI 6917 GCTAGC G^CTAGC NruI 226 TCGCGA TCG^CGA PmaCI 3219 CACGTG CAC^GTG PmlI 3219 CACGTG CAC^GTG RsrII 290 CGGTCCG CG^G[AT]CCG SacII 141 CCGCGG CCGC^GG SbfI 2376 CCTGCAGG CCTGCA^GG SexAI 4655 ACCAGGT A^CC[AT]GGT SfiI 287 GGCCGGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3464 GCATGC GCATG^C Sse8387I 2376 CCTGCAGG CCTGCA^GG SspI 5115 AATATT AAT^ATT XbaI 5636 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AflII 2646 CTTAAG C^TTAAG 7179 CTTAAG Aor51HI 4856 AGCGCT AGC^GCT 7231 AGCGCT Asp700I 1566 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2562 GAACCAGTTC BamHI 476 GGATCC G^GATCC 3854 GGATCC BcgI 3879 CGACCACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1118 GCAATGGCATCG BfrI 2646 CTTAAG C^TTAAG 7179 CTTAAG BsaBI 1905 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7256 GATTGGAATC BsaI 1177 GAGACC GGTCTC(1/5) 3831 GGTCTC DraI 1933 TTTAAA TTT^AAA 5012 TTTAAA DrdI 2476 GACACCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2817 GACCTCCCTGTC Eco47III 4856 AGCGCT AGC^GCT 7231 AGCGCT MamI 1905 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7256 GATTGGAATC PshAI 2585 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 7346 GACCTGAGTC SacI 301 GAGCTC GAGCT^C 6746 GAGCTC SapI 147 GCTCTTC GCTCTTC(1/4) 963 GCTCTTC ScaI 1727 AGTACT AGT^ACT 5316 AGTACT XmnI 1566 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2562 GAACCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 149 enzymes) # ######################################### AccI AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI