##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AgeI Aor51HI ApaI
AscI AviII AvrII BbeI BbrPI
BcgI BglI BlnI BlpI Bpu1102I
BsiEI BsiWI BspDI BspHI BsrFI
BssHII Bst1107I BstZ17I CelII Cfr10I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoNI FseI FspI KasI
KspI MluI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
Sse8387I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1838 GGTACC G^GTACC
AccI 7086 GTAGAC GT^[AC][GT]AC
AccIII 812 TCCGGA T^CCGGA
AcyI 340 GGCGTC G[GA]^CG[CT]C
AhdI 6501 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1252 GTGCAC G^TGCAC
ApaLI 1252 GTGCAC G^TGCAC
Asp718I 1838 GGTACC G^GTACC
AspEI 6501 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 409 GGATCC G^GATCC
BbvCI 1763 GCTGAGG CCTCAGC(-5/-2)
BglII 4145 AGATCT A^GATCT
BsaHI 340 GGCGTC G[GA]^CG[CT]C
BseAI 812 TCCGGA T^CCGGA
BsgI 2530 CTGCAC GTGCAG(16/14)
BsmBI 3535 GAGACG CGTCTC(1/5)
BspEI 812 TCCGGA T^CCGGA
BsrBI 808 CCGCTC CCGCTC(-3/-3)
BstBI 3505 TTCGAA TT^CGAA
DraIII 2859 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 325 GACTCCGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6501 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2679 GAATTC G^AATTC
HaeII 102 GGCGCT [GA]GCGC^[CT]
Hin1I 340 GGCGTC G[GA]^CG[CT]C
HincII 782 GTTAAC GT[CT]^[GA]AC
HindII 782 GTTAAC GT[CT]^[GA]AC
HpaI 782 GTTAAC GTT^AAC
KpnI 1838 GGTACC GGTAC^C
MfeI 4600 CAATTG C^AATTG
MroI 812 TCCGGA T^CCGGA
MunI 4600 CAATTG C^AATTG
NheI 6943 GCTAGC G^CTAGC
NspV 3505 TTCGAA TT^CGAA
PshAI 2611 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 953 GAGCTC GAGCT^C
SfuI 3505 TTCGAA TT^CGAA
SnaBI 4024 TACGTA TAC^GTA
StuI 6715 AGGCCT AGG^CCT
SwaI 5756 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 970 CTTAAG C^TTAAG
2631 CTTAAG
AspI 176 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
325 GACTCCGTC
AvaI 569 CTCGGG C^[CT]CG[GA]G
933 CTCGGG
BalI 4083 TGGCCA TGG^CCA
6847 TGGCCA
BfrI 970 CTTAAG C^TTAAG
2631 CTTAAG
BsaAI 3993 CACGTA [CT]AC^GT[GA]
4024 TACGTA
BsaI 378 GAGACC GGTCTC(1/5)
6916 GGTCTC
BsoBI 569 CTCGGG C^[CT]CG[GA]G
933 CTCGGG
Bsp1407I 5096 TGTACA T^GTACA
5594 TGTACA
BspMI 549 ACCTGC ACCTGC(4/8)
2534 ACCTGC
BsrDI 2136 GCAATG GCAATG(2/0)
5995 GCAATG
BsrGI 5096 TGTACA T^GTACA
5594 TGTACA
BssSI 243 CACGAG CACGAG(-5/-1)
488 CACGAG
DraII 1404 AGGACCT [GA]G^G[ACGT]CC[CT]
6854 AGGTCCT
EcoO109I 1404 AGGACCT [GA]G^G[ACGT]CC[CT]
6854 AGGTCCT
EcoT22I 6391 ATGCAT ATGCA^T
6831 ATGCAT
HindIII 698 AAGCTT A^AGCTT
2029 AAGCTT
MluNI 4083 TGGCCA TGG^CCA
6847 TGGCCA
MscI 4083 TGGCCA TGG^CCA
6847 TGGCCA
NsiI 6391 ATGCAT ATGCA^T
6831 ATGCAT
PflFI 176 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
325 GACTCCGTC
PflMI 1842 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6306 CCACATCTTGG
PpuMI 1404 AGGACCT [GA]G^G[AT]CC[CT]
6854 AGGTCCT
SapI 129 GAAGAGC GCTCTTC(1/4)
955 GCTCTTC
SpeI 4339 ACTAGT A^CTAGT
6641 ACTAGT
SphI 922 GCATGC GCATG^C
964 GCATGC
SspBI 5096 TGTACA T^GTACA
5594 TGTACA
Tth111I 176 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
325 GACTCCGTC
Van91I 1842 CCAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6306 CCACATCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI Eco81I EcoO65I
EcoRII EcoRV EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI