##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbeI BlnI BsiWI
BsrBI BsrFI BssHII Bst1107I BstZ17I
Cfr10I CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI
PinAI PmeI PshBI Psp1406I RsrII
SacII SalI ScaI SfiI SfoI
SgrAI SmaI SnaBI StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 4510 GTAGAC GT^[AC][GT]AC
AccIII 3635 TCCGGA T^CCGGA
AcyI 1832 GGCGTC G[GA]^CG[CT]C
Alw44I 6778 GTGCAC G^TGCAC
AocI 2880 CCTCAGG CC^T[ACGT]AGG
ApaLI 6778 GTGCAC G^TGCAC
BbrPI 3064 CACGTG CAC^GTG
BsaHI 1832 GGCGTC G[GA]^CG[CT]C
BseAI 3635 TCCGGA T^CCGGA
BsmBI 2922 GAGACG CGTCTC(1/5)
Bsp1407I 6757 TGTACA T^GTACA
BspDI 1765 ATCGAT AT^CGAT
BspEI 3635 TCCGGA T^CCGGA
BsrGI 6757 TGTACA T^GTACA
BssSI 1556 CACGAG CACGAG(-5/-1)
BstBI 5561 TTCGAA TT^CGAA
BstEII 7107 GGTCACC G^GT[ACGT]ACC
BstPI 7107 GGTCACC G^GT[ACGT]ACC
Bsu36I 2880 CCTCAGG CC^T[ACGT]AGG
ClaI 1765 ATCGAT AT^CGAT
DraIII 58 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2880 CCTCAGG CC^T[ACGT]AGG
EcoO65I 7107 GGTCACC G^GT[ACGT]ACC
EcoT22I 5466 ATGCAT ATGCA^T
Hin1I 1832 GGCGTC G[GA]^CG[CT]C
HpaI 5992 GTTAAC GTT^AAC
MfeI 1000 CAATTG C^AATTG
MroI 3635 TCCGGA T^CCGGA
MunI 1000 CAATTG C^AATTG
NsiI 5466 ATGCAT ATGCA^T
NspV 5561 TTCGAA TT^CGAA
PaeR7I 4601 CTCGAG C^TCGAG
PmaCI 3064 CACGTG CAC^GTG
PmlI 3064 CACGTG CAC^GTG
PshAI 517 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4683 CGATCG CGAT^CG
SbfI 3876 CCTGCAGG CCTGCA^GG
SfuI 5561 TTCGAA TT^CGAA
SphI 1274 GCATGC GCATG^C
Sse8387I 3876 CCTGCAGG CCTGCA^GG
SspBI 6757 TGTACA T^GTACA
SspI 6831 AATATT AAT^ATT
XbaI 4961 TCTAGA T^CTAGA
XcmI 4136 CCACTCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4601 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 2416 GGGCCC GGGCC^C
5525 GGGCCC
BalI 1304 TGGCCA TGG^CCA
2868 TGGCCA
BbsI 1569 GTCTTC GAAGAC(2/6)
2103 GTCTTC
BciVI 963 GGATAC GTATCC(6/5)
5297 GTATCC
BpuAI 1569 GTCTTC GAAGAC(2/6)
2103 GTCTTC
BsaAI 3064 CACGTG [CT]AC^GT[GA]
6692 TACGTG
BsaBI 4678 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6763 GATTATAATC
BsiEI 3198 CGACCG CG[GA][CT]^CG
4683 CGATCG
BspHI 3512 TCATGA T^CATGA
6474 TCATGA
BstAPI 3618 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3933 GCAGACCCTGC
EcoRI 4056 GAATTC G^AATTC
5855 GAATTC
EcoRV 5241 GATATC GAT^ATC
6460 GATATC
MamI 4678 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6763 GATTATAATC
MluNI 1304 TGGCCA TGG^CCA
2868 TGGCCA
MscI 1304 TGGCCA TGG^CCA
2868 TGGCCA
RcaI 3512 TCATGA T^CATGA
6474 TCATGA
SacI 2361 GAGCTC GAGCT^C
3348 GAGCTC
SapI 3253 GAAGAGC GCTCTTC(1/4)
3663 GCTCTTC
SexAI 2012 ACCTGGT A^CC[AT]GGT
3307 ACCAGGT
SpeI 432 ACTAGT A^CTAGT
648 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AhdI AluI AlwI AlwNI
ApoI Asp700I AspEI AspHI AspI
AvaI AvaII BanI BanII BbvCI
BbvI BcgI BclI BcnI BfaI
BfrI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmnI