##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
Aor51HI     ApaI        AscI        AviII       BanII       
BbsI        BcgI        BfrI        BpuAI       BsaI        
BseAI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrGI       BssHII      BstAPI      
BstBI       BstEII      BstPI       BstUI       ClaI        
CpoI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRV       FseI        FspI        
KspI        MluI        MroI        MvnI        NaeI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PmeI        Psp1406I    
PvuI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SphI        Sse8387I    SspBI       XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       1587  GGTACC               G^GTACC
AgeI         1158  ACCGGT               A^CCGGT
Asp718I      1587  GGTACC               G^GTACC
AspI         5186  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1717  CCCGAG               C^[CT]CG[GA]G
BamHI        1686  GGATCC               G^GATCC
BbeI         1370  GGCGCC               GGCGC^C
BbvCI        1926  GCTGAGG              CCTCAGC(-5/-2)
BlpI         6837  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     6837  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1158  ACCGGT               [AT]^CCGG[AT]
BsiEI        5185  CGACCG               CG[GA][CT]^CG
BsoBI        1717  CCCGAG               C^[CT]CG[GA]G
BspHI        1794  TCATGA               T^CATGA
BspMI        1350  ACCTGC               ACCTGC(4/8)
BssSI        1459  CACGAG               CACGAG(-5/-1)
CelII        6837  GCTGAGC              GC^T[ACGT]AGC
CfoI         1371  GCGC                 GCG^C
EcoNI        5922  CCTAGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         1530  CCCGC                CCCGC(4/6)
HaeII        1370  GGCGCC               [GA]GCGC^[CT]
HhaI         1371  GCGC                 GCG^C
HinP1I       1371  GCGC                 G^CGC
HpaI         7032  GTTAAC               GTT^AAC
KasI         1370  GGCGCC               G^GCGCC
KpnI         1587  GGTACC               GGTAC^C
NarI         1370  GGCGCC               GG^CGCC
PflFI        5186  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1158  ACCGGT               A^CCGGT
PpuMI        1445  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         1794  TCATGA               T^CATGA
SalI          977  GTCGAC               G^TCGAC
SfoI         1370  GGCGCC               GGC^GCC
SnaBI         150  TACGTA               TAC^GTA
StuI         1540  AGGCCT               AGG^CCT
Tth111I      5186  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AhdI         4296  GACTATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4451  GACAGTTTGTC         
Alw44I       5672  GTGCAC               G^TGCAC
             7421  GTGCAC              
AlwNI         549  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             5731  CAGATTCTG           
AocI          872  CCTCAGG              CC^T[ACGT]AGG
             2907  CCTGAGG             
ApaLI        5672  GTGCAC               G^TGCAC
             7421  GTGCAC              
AspEI        4296  GACTATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4451  GACAGTTTGTC         
AvrII        1438  CCTAGG               C^CTAGG
             5922  CCTAGG              
BbrPI        5712  CACGTG               CAC^GTG
             7455  CACGTG              
BclI         4594  TGATCA               T^GATCA
             6798  TGATCA              
BlnI         1438  CCTAGG               C^CTAGG
             5922  CCTAGG              
BsrDI        4306  CATTGC               GCAATG(2/0)
              840  GCAATG              
Bst1107I     3789  GTATAC               GTA^TAC
             5807  GTATAC              
BstZ17I      3789  GTATAC               GTA^TAC
             5807  GTATAC              
Bsu36I        872  CCTCAGG              CC^T[ACGT]AGG
             2907  CCTGAGG             
DraII        1445  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3054  GGGGCCT             
DraIII       2035  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3760  CACTGTGTG           
DrdI          968  GACTCACCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3252  GACTGTTGCGTC        
Eam1105I     4296  GACTATTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4451  GACAGTTTGTC         
Eco81I        872  CCTCAGG              CC^T[ACGT]AGG
             2907  CCTGAGG             
EcoO109I     1445  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             3054  GGGGCCT             
FbaI         4594  TGATCA               T^GATCA
             6798  TGATCA              
MfeI         2247  CAATTG               C^AATTG
             4834  CAATTG              
MunI         2247  CAATTG               C^AATTG
             4834  CAATTG              
PmaCI        5712  CACGTG               CAC^GTG
             7455  CACGTG              
PmlI         5712  CACGTG               CAC^GTG
             7455  CACGTG              
PshAI         712  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3113  GACTAAGGTC          
SexAI         767  ACCAGGT              A^CC[AT]GGT
             7462  ACCAGGT             
SmlI         5534  CTCAAG               C^T[CT][GA]AG
             6143  CTCAAG              
SpeI         6653  ACTAGT               A^CTAGT
             7610  ACTAGT              
SspI          510  AATATT               AAT^ATT
             7408  AATATT              
SwaI         3513  ATTTAAAT             ATTT^AAAT
             4230  ATTTAAAT            
XbaI          575  TCTAGA               T^CTAGA
             3546  TCTAGA              
XcmI          750  CCAAAAACAAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3934  CCAGAAATAATATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  117 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApoI        AseI        
AsnI        Asp700I     AspHI       AvaII       BalI        
BanI        BbvI        BciVI       BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BsaAI       
BsaBI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspLU11I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DraI        EaeI        EarI        EcoRI       EcoRII      
EcoT14I     EcoT22I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PshBI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI