################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccII AccIII AclI AflII Aor51HI ApaI AscI AviII BanII BbsI BcgI BfrI BpuAI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI ClaI CpoI DsaI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI KspI MluI MroI MvnI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PmeI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SfiI SfuI SgrAI SmaI SphI Sse8387I SspBI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 1587 GGTACC G^GTACC AgeI 1158 ACCGGT A^CCGGT Asp718I 1587 GGTACC G^GTACC AspI 5186 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1717 CCCGAG C^[CT]CG[GA]G BamHI 1686 GGATCC G^GATCC BbeI 1370 GGCGCC GGCGC^C BbvCI 1926 GCTGAGG CCTCAGC(-5/-2) BlpI 6837 GCTGAGC GC^T[ACGT]AGC Bpu1102I 6837 GCTGAGC GC^T[ACGT]AGC BsaWI 1158 ACCGGT [AT]^CCGG[AT] BsiEI 5185 CGACCG CG[GA][CT]^CG BsoBI 1717 CCCGAG C^[CT]CG[GA]G BspHI 1794 TCATGA T^CATGA BspMI 1350 ACCTGC ACCTGC(4/8) BssSI 1459 CACGAG CACGAG(-5/-1) CelII 6837 GCTGAGC GC^T[ACGT]AGC CfoI 1371 GCGC GCG^C EcoNI 5922 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 1530 CCCGC CCCGC(4/6) HaeII 1370 GGCGCC [GA]GCGC^[CT] HhaI 1371 GCGC GCG^C HinP1I 1371 GCGC G^CGC HpaI 7032 GTTAAC GTT^AAC KasI 1370 GGCGCC G^GCGCC KpnI 1587 GGTACC GGTAC^C NarI 1370 GGCGCC GG^CGCC PflFI 5186 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1158 ACCGGT A^CCGGT PpuMI 1445 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 1794 TCATGA T^CATGA SalI 977 GTCGAC G^TCGAC SfoI 1370 GGCGCC GGC^GCC SnaBI 150 TACGTA TAC^GTA StuI 1540 AGGCCT AGG^CCT Tth111I 5186 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AhdI 4296 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4451 GACAGTTTGTC Alw44I 5672 GTGCAC G^TGCAC 7421 GTGCAC AlwNI 549 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 5731 CAGATTCTG AocI 872 CCTCAGG CC^T[ACGT]AGG 2907 CCTGAGG ApaLI 5672 GTGCAC G^TGCAC 7421 GTGCAC AspEI 4296 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4451 GACAGTTTGTC AvrII 1438 CCTAGG C^CTAGG 5922 CCTAGG BbrPI 5712 CACGTG CAC^GTG 7455 CACGTG BclI 4594 TGATCA T^GATCA 6798 TGATCA BlnI 1438 CCTAGG C^CTAGG 5922 CCTAGG BsrDI 4306 CATTGC GCAATG(2/0) 840 GCAATG Bst1107I 3789 GTATAC GTA^TAC 5807 GTATAC BstZ17I 3789 GTATAC GTA^TAC 5807 GTATAC Bsu36I 872 CCTCAGG CC^T[ACGT]AGG 2907 CCTGAGG DraII 1445 AGGTCCT [GA]G^G[ACGT]CC[CT] 3054 GGGGCCT DraIII 2035 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 3760 CACTGTGTG DrdI 968 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3252 GACTGTTGCGTC Eam1105I 4296 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4451 GACAGTTTGTC Eco81I 872 CCTCAGG CC^T[ACGT]AGG 2907 CCTGAGG EcoO109I 1445 AGGTCCT [GA]G^G[ACGT]CC[CT] 3054 GGGGCCT FbaI 4594 TGATCA T^GATCA 6798 TGATCA MfeI 2247 CAATTG C^AATTG 4834 CAATTG MunI 2247 CAATTG C^AATTG 4834 CAATTG PmaCI 5712 CACGTG CAC^GTG 7455 CACGTG PmlI 5712 CACGTG CAC^GTG 7455 CACGTG PshAI 712 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3113 GACTAAGGTC SexAI 767 ACCAGGT A^CC[AT]GGT 7462 ACCAGGT SmlI 5534 CTCAAG C^T[CT][GA]AG 6143 CTCAAG SpeI 6653 ACTAGT A^CTAGT 7610 ACTAGT SspI 510 AATATT AAT^ATT 7408 AATATT SwaI 3513 ATTTAAAT ATTT^AAAT 4230 ATTTAAAT XbaI 575 TCTAGA T^CTAGA 3546 TCTAGA XcmI 750 CCAAAAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3934 CCAGAAATAATATGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BalI BanI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BsaAI BsaBI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI