##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII Acc65I AgeI AhdI AocI
AscI Asp718I AspEI BclI BsiEI
BsiWI BsrFI BssHII BssSI Bst1107I
BstZ17I Bsu36I Cfr10I CpoI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoT22I FbaI FseI KpnI KspI
MluI NaeI NgoMIV NotI NruI
NsiI PinAI PmeI PvuI RsrII
SacII SalI SbfI SfiI SgrAI
SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 4363 GTCTAC GT^[AC][GT]AC
AccIII 5404 TCCGGA T^CCGGA
AclI 5370 AACGTT AA^CGTT
AflII 4682 CTTAAG C^TTAAG
Aor51HI 1015 AGCGCT AGC^GCT
AviII 2658 TGCGCA TGC^GCA
BamHI 4576 GGATCC G^GATCC
BbeI 3427 GGCGCC GGCGC^C
BbrPI 4001 CACGTG CAC^GTG
BfrI 4682 CTTAAG C^TTAAG
BsaAI 4001 CACGTG [CT]AC^GT[GA]
BsaI 5826 GAGACC GGTCTC(1/5)
BseAI 5404 TCCGGA T^CCGGA
BspEI 5404 TCCGGA T^CCGGA
BspHI 2379 TCATGA T^CATGA
BspMI 3491 GCAGGT ACCTGC(4/8)
BstBI 4145 TTCGAA TT^CGAA
DraIII 6865 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1015 AGCGCT AGC^GCT
FspI 2658 TGCGCA TGC^GCA
HpaI 440 GTTAAC GTT^AAC
KasI 3427 GGCGCC G^GCGCC
MfeI 6412 CAATTG C^AATTG
MroI 5404 TCCGGA T^CCGGA
MunI 6412 CAATTG C^AATTG
NarI 3427 GGCGCC GG^CGCC
NcoI 729 CCATGG C^CATGG
NdeI 936 CATATG CA^TATG
NheI 3053 GCTAGC G^CTAGC
NspV 4145 TTCGAA TT^CGAA
PacI 326 TTAATTAA TTAAT^TAA
PflMI 1721 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4001 CACGTG CAC^GTG
PmlI 4001 CACGTG CAC^GTG
Psp1406I 5370 AACGTT AA^CGTT
PvuII 6209 CAGCTG CAG^CTG
RcaI 2379 TCATGA T^CATGA
SexAI 117 ACCAGGT A^CC[AT]GGT
SfoI 3427 GGCGCC GGC^GCC
SfuI 4145 TTCGAA TT^CGAA
SmaI 4358 CCCGGG CCC^GGG
Van91I 1721 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 6833 TCTAGA T^CTAGA
XcmI 1717 CCACCCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4358 CCCGGG C^CCGGG
XmaI 4358 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 3427 GGCGCC G[GA]^CG[CT]C
4120 GACGCC
AflIII 2117 ACATGT A^C[GA][CT]GT
6572 ACATGT
Alw44I 407 GTGCAC G^TGCAC
5927 GTGCAC
ApaLI 407 GTGCAC G^TGCAC
5927 GTGCAC
AspI 2501 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2522 GACACTGTC
AvrII 3422 CCTAGG C^CTAGG
5918 CCTAGG
BcgI 32 CGACGCGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2012 GCAACCCTCTCG
BciVI 964 GGATAC GTATCC(6/5)
2772 GGATAC
BglII 4382 AGATCT A^GATCT
5758 AGATCT
BlnI 3422 CCTAGG C^CTAGG
5918 CCTAGG
BlpI 4835 GCTGAGC GC^T[ACGT]AGC
6280 GCTTAGC
Bpu1102I 4835 GCTGAGC GC^T[ACGT]AGC
6280 GCTTAGC
BsaBI 740 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6856 GATGTTTATC
BsaHI 3427 GGCGCC G[GA]^CG[CT]C
4120 GACGCC
BsaWI 3087 ACCGGA [AT]^CCGG[AT]
5404 TCCGGA
BseRI 1874 CTCCTC GAGGAG(10/8)
3733 CTCCTC
BsmI 5120 GAATGC GAATGC(1/-1)
5463 GCATTC
Bsp1407I 561 TGTACA T^GTACA
7225 TGTACA
BspDI 2474 ATCGAT AT^CGAT
4529 ATCGAT
BspLU11I 2117 ACATGT A^CATGT
6572 ACATGT
BsrBI 3398 CCGCTC CCGCTC(-3/-3)
89 GAGCGG
BsrGI 561 TGTACA T^GTACA
7225 TGTACA
BstAPI 913 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5894 GCAAACCCTGC
BstXI 575 CCAATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1533 CCATTTGTGTGG
CelII 4835 GCTGAGC GC^T[ACGT]AGC
6280 GCTTAGC
ClaI 2474 ATCGAT AT^CGAT
4529 ATCGAT
DrdI 5514 GACAGAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6093 GACGGGGCTGTC
DsaI 729 CCATGG C^C[GA][CT]GG
6147 CCGTGG
EcoRV 169 GATATC GAT^ATC
1115 GATATC
Hin1I 3427 GGCGCC G[GA]^CG[CT]C
4120 GACGCC
MamI 740 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6856 GATGTTTATC
PaeR7I 3279 CTCGAG C^TCGAG
3736 CTCGAG
PflFI 2501 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2522 GACACTGTC
PshAI 1434 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2954 GACATCAGTC
PstI 81 CTGCAG CTGCA^G
5615 CTGCAG
SacI 4106 GAGCTC GAGCT^C
5408 GAGCTC
ScaI 3973 AGTACT AGT^ACT
6683 AGTACT
SphI 5162 GCATGC GCATG^C
7258 GCATGC
SspBI 561 TGTACA T^GTACA
7225 TGTACA
StuI 5297 AGGCCT AGG^CCT
6187 AGGCCT
Tth111I 2501 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2522 GACACTGTC
XhoI 3279 CTCGAG C^TCGAG
3736 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI