################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## Acc65I AccIII AclI AflII AscI AseI AsnI Asp718I AviII BbeI BciVI BclI BfrI BsaBI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoT22I FbaI FseI FspI HpaI KasI KpnI MamI MroI NarI NcoI NdeI NotI NruI NsiI PacI PmeI PshBI Psp1406I PvuI RsrII SalI SexAI SfiI SfoI SnaBI SpeI SwaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AgeI 5364 ACCGGT A^CCGGT ApaI 1343 GGGCCC GGGCC^C Asp700I 6158 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 1353 GGATCC G^GATCC BcgI 1889 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiEI 803 CGACCG CG[GA][CT]^CG Bsp1407I 2726 TGTACA T^GTACA BsrGI 2726 TGTACA T^GTACA BstBI 2164 TTCGAA TT^CGAA EcoRI 335 GAATTC G^AATTC EcoRV 7244 GATATC GAT^ATC KspI 1193 CCGCGG CCGC^GG MfeI 5134 CAATTG C^AATTG MunI 5134 CAATTG C^AATTG NaeI 2120 GCCGGC GCC^GGC NgoMIV 2120 GCCGGC G^CCGGC NheI 7230 GCTAGC G^CTAGC NspV 2164 TTCGAA TT^CGAA PinAI 5364 ACCGGT A^CCGGT PshAI 810 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 756 GAGCTC GAGCT^C SacII 1193 CCGCGG CCGC^GG SapI 6158 GAAGAGC GCTCTTC(1/4) SbfI 2283 CCTGCAGG CCTGCA^GG ScaI 2036 AGTACT AGT^ACT SfuI 2164 TTCGAA TT^CGAA SgrAI 5363 CACCGGTG C[GA]^CCGG[CT]G Sse8387I 2283 CCTGCAGG CCTGCA^GG SspBI 2726 TGTACA T^GTACA SspI 1249 AATATT AAT^ATT XmnI 6158 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 2951 GTAGAC GT^[AC][GT]AC 3944 GTCTAC BglI 3802 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5140 GCCTGGCAGGC BglII 2396 AGATCT A^GATCT 7313 AGATCT BsaI 6740 GAGACC GGTCTC(1/5) 2224 GGTCTC BsmI 2167 GAATGC GAATGC(1/-1) 4748 GAATGC BspHI 5590 TCATGA T^CATGA 6353 TCATGA BspLU11I 2772 ACATGT A^CATGT 4309 ACATGT BsrBI 565 CCGCTC CCGCTC(-3/-3) 3690 CCGCTC BsrDI 1689 CATTGC GCAATG(2/0) 3445 GCAATG DrdI 1714 GACTGTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5280 GACTGGAGGGTC HindIII 1057 AAGCTT A^AGCTT 4316 AAGCTT MluI 2177 ACGCGT A^CGCGT 3573 ACGCGT PaeR7I 713 CTCGAG C^TCGAG 1901 CTCGAG RcaI 5590 TCATGA T^CATGA 6353 TCATGA XbaI 7042 TCTAGA T^CTAGA 7290 TCTAGA XhoI 713 CTCGAG C^TCGAG 1901 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 154 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AflIII AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AspEI AspHI AspI AvaI AvaII AvrII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI