##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AscI        
AseI        AsnI        Asp718I     AviII       BbeI        
BciVI       BclI        BfrI        BsaBI       BseAI       
BsiWI       BspDI       BspEI       BssHII      Bst1107I    
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EagI        EclXI       Eco52I      EcoO65I     EcoT22I     
FbaI        FseI        FspI        HpaI        KasI        
KpnI        MamI        MroI        NarI        NcoI        
NdeI        NotI        NruI        NsiI        PacI        
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SalI        SexAI       SfiI        SfoI        SnaBI       
SpeI        SwaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AgeI         5364  ACCGGT               A^CCGGT
ApaI         1343  GGGCCC               GGGCC^C
Asp700I      6158  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI        1353  GGATCC               G^GATCC
BcgI         1889  GCAGGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI         803  CGACCG               CG[GA][CT]^CG
Bsp1407I     2726  TGTACA               T^GTACA
BsrGI        2726  TGTACA               T^GTACA
BstBI        2164  TTCGAA               TT^CGAA
EcoRI         335  GAATTC               G^AATTC
EcoRV        7244  GATATC               GAT^ATC
KspI         1193  CCGCGG               CCGC^GG
MfeI         5134  CAATTG               C^AATTG
MunI         5134  CAATTG               C^AATTG
NaeI         2120  GCCGGC               GCC^GGC
NgoMIV       2120  GCCGGC               G^CCGGC
NheI         7230  GCTAGC               G^CTAGC
NspV         2164  TTCGAA               TT^CGAA
PinAI        5364  ACCGGT               A^CCGGT
PshAI         810  GACACCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          756  GAGCTC               GAGCT^C
SacII        1193  CCGCGG               CCGC^GG
SapI         6158  GAAGAGC              GCTCTTC(1/4)
SbfI         2283  CCTGCAGG             CCTGCA^GG
ScaI         2036  AGTACT               AGT^ACT
SfuI         2164  TTCGAA               TT^CGAA
SgrAI        5363  CACCGGTG             C[GA]^CCGG[CT]G
Sse8387I     2283  CCTGCAGG             CCTGCA^GG
SspBI        2726  TGTACA               T^GTACA
SspI         1249  AATATT               AAT^ATT
XmnI         6158  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AccI         2951  GTAGAC               GT^[AC][GT]AC
             3944  GTCTAC              
BglI         3802  GCCAGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5140  GCCTGGCAGGC         
BglII        2396  AGATCT               A^GATCT
             7313  AGATCT              
BsaI         6740  GAGACC               GGTCTC(1/5)
             2224  GGTCTC              
BsmI         2167  GAATGC               GAATGC(1/-1)
             4748  GAATGC              
BspHI        5590  TCATGA               T^CATGA
             6353  TCATGA              
BspLU11I     2772  ACATGT               A^CATGT
             4309  ACATGT              
BsrBI         565  CCGCTC               CCGCTC(-3/-3)
             3690  CCGCTC              
BsrDI        1689  CATTGC               GCAATG(2/0)
             3445  GCAATG              
DrdI         1714  GACTGTGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5280  GACTGGAGGGTC        
HindIII      1057  AAGCTT               A^AGCTT
             4316  AAGCTT              
MluI         2177  ACGCGT               A^CGCGT
             3573  ACGCGT              
PaeR7I        713  CTCGAG               C^TCGAG
             1901  CTCGAG              
RcaI         5590  TCATGA               T^CATGA
             6353  TCATGA              
XbaI         7042  TCTAGA               T^CTAGA
             7290  TCTAGA              
XhoI          713  CTCGAG               C^TCGAG
             1901  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  154 enzymes) #
#########################################

AatII       AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AspEI       AspHI       AspI        AvaI        
AvaII       AvrII       BalI        BanI        BanII       
BbrPI       BbsI        BbvCI       BbvI        BcnI        
BfaI        BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
Eam1105I    EarI        Eco47III    Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SphI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI