##################################
# NO CUT ENZYMES (   49 enzymes) #
##################################

AclI        AgeI        Aor51HI     AscI        BbeI        
BbrPI       BsaAI       BsiEI       BsiWI       BssHII      
Bst1107I    BstZ17I     DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       FseI        
HaeII       HindIII     KasI        KspI        MluI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        SacII       
SbfI        SexAI       SfoI        SgrAI       SnaBI       
Sse8387I    SspI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       6737  GGTACC               G^GTACC
AccIII       3220  TCCGGA               T^CCGGA
AflII        6321  CTTAAG               C^TTAAG
AocI         6800  CCTCAGG              CC^T[ACGT]AGG
ApaI         2140  GGGCCC               GGGCC^C
AseI         3099  ATTAAT               AT^TAAT
AsnI         3099  ATTAAT               AT^TAAT
Asp718I      6737  GGTACC               G^GTACC
AviII        3175  TGCGCA               TGC^GCA
BcgI         1552  CGAAAGCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4476  GTATCC               GTATCC(6/5)
BfrI         6321  CTTAAG               C^TTAAG
BglI         2631  GCCAACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI        3220  TCCGGA               [AT]^CCGG[AT]
BseAI        3220  TCCGGA               T^CCGGA
BsmBI        1548  GAGACG               CGTCTC(1/5)
BspDI        3666  ATCGAT               AT^CGAT
BspEI        3220  TCCGGA               T^CCGGA
BspLU11I     1771  ACATGT               A^CATGT
BssSI        3969  CTCGTG               CACGAG(-5/-1)
Bsu36I       6800  CCTCAGG              CC^T[ACGT]AGG
ClaI         3666  ATCGAT               AT^CGAT
CpoI         3741  CGGTCCG              CG^G[AT]CCG
Eco81I       6800  CCTCAGG              CC^T[ACGT]AGG
FspI         3175  TGCGCA               TGC^GCA
HpaI         5557  GTTAAC               GTT^AAC
KpnI         6737  GGTACC               GGTAC^C
MfeI         3533  CAATTG               C^AATTG
MroI         3220  TCCGGA               T^CCGGA
MunI         3533  CAATTG               C^AATTG
PshAI        3434  GACAGCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3099  ATTAAT               AT^TAAT
RsrII        3741  CGGTCCG              CG^G[AT]CCG
SacI         4823  GAGCTC               GAGCT^C
SalI         4250  GTCGAC               G^TCGAC
SfiI         2630  GGCCAACGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         2206  CCCGGG               CCC^GGG
SpeI         2885  ACTAGT               A^CTAGT
SphI         3904  GCATGC               GCATG^C
XmaCI        2206  CCCGGG               C^CCGGG
XmaI         2206  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AatII          82  GACGTC               GACGT^C
             2519  GACGTC              
AccI         3033  GTCTAC               GT^[AC][GT]AC
             4250  GTCGAC              
AccII        3813  CGCG                 CG^CG
             3994  CGCG                
AcyI           82  GACGTC               G[GA]^CG[CT]C
             2519  GACGTC              
AhdI         5782  GACCATATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7105  GACCAACCGTC         
AspEI        5782  GACCATATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7105  GACCAACCGTC         
AvrII         593  CCTAGG               C^CTAGG
             7158  CCTAGG              
BglII         653  AGATCT               A^GATCT
             6807  AGATCT              
BlnI          593  CCTAGG               C^CTAGG
             7158  CCTAGG              
BlpI         2055  GCTAAGC              GC^T[ACGT]AGC
             2178  GCTCAGC             
Bpu1102I     2055  GCTAAGC              GC^T[ACGT]AGC
             2178  GCTCAGC             
BsaBI        1640  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1808  GATCCCCATC          
BsaHI          82  GACGTC               G[GA]^CG[CT]C
             2519  GACGTC              
Bsp1407I     4486  TGTACA               T^GTACA
             5076  TGTACA              
BspHI        1060  TCATGA               T^CATGA
             3817  TCATGA              
BsrBI        3758  CCGCTC               CCGCTC(-3/-3)
             1602  GAGCGG              
BsrGI        4486  TGTACA               T^GTACA
             5076  TGTACA              
BstBI        1129  TTCGAA               TT^CGAA
             1359  TTCGAA              
BstEII       2486  GGTCACC              G^GT[ACGT]ACC
             7187  GGTCACC             
BstPI        2486  GGTCACC              G^GT[ACGT]ACC
             7187  GGTCACC             
BstUI        3813  CGCG                 CG^CG
             3994  CGCG                
CelII        2055  GCTAAGC              GC^T[ACGT]AGC
             2178  GCTCAGC             
DraI         5839  TTTAAA               TTT^AAA
             5864  TTTAAA              
Eam1105I     5782  GACCATATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             7105  GACCAACCGTC         
EcoO65I      2486  GGTCACC              G^GT[ACGT]ACC
             7187  GGTCACC             
HgaI         3814  GCGTC                GACGC(5/10)
             4501  GCGTC               
Hin1I          82  GACGTC               G[GA]^CG[CT]C
             2519  GACGTC              
MamI         1640  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1808  GATCCCCATC          
MvnI         3813  CGCG                 CG^CG
             3994  CGCG                
NcoI         1000  CCATGG               C^CATGG
             5190  CCATGG              
NspV         1129  TTCGAA               TT^CGAA
             1359  TTCGAA              
RcaI         1060  TCATGA               T^CATGA
             3817  TCATGA              
ScaI          384  AGTACT               AGT^ACT
             4862  AGTACT              
SfuI         1129  TTCGAA               TT^CGAA
             1359  TTCGAA              
SspBI        4486  TGTACA               T^GTACA
             5076  TGTACA              
StuI         2808  AGGCCT               AGG^CCT
             4734  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
Asp700I     AspHI       AspI        AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BclI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PflFI       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoII       XmnI