################################## # NO CUT ENZYMES ( 49 enzymes) # ################################## AclI AgeI Aor51HI AscI BbeI BbrPI BsaAI BsiEI BsiWI BssHII Bst1107I BstZ17I DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV FseI HaeII HindIII KasI KspI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI SacII SbfI SexAI SfoI SgrAI SnaBI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 6737 GGTACC G^GTACC AccIII 3220 TCCGGA T^CCGGA AflII 6321 CTTAAG C^TTAAG AocI 6800 CCTCAGG CC^T[ACGT]AGG ApaI 2140 GGGCCC GGGCC^C AseI 3099 ATTAAT AT^TAAT AsnI 3099 ATTAAT AT^TAAT Asp718I 6737 GGTACC G^GTACC AviII 3175 TGCGCA TGC^GCA BcgI 1552 CGAAAGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4476 GTATCC GTATCC(6/5) BfrI 6321 CTTAAG C^TTAAG BglI 2631 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 3220 TCCGGA [AT]^CCGG[AT] BseAI 3220 TCCGGA T^CCGGA BsmBI 1548 GAGACG CGTCTC(1/5) BspDI 3666 ATCGAT AT^CGAT BspEI 3220 TCCGGA T^CCGGA BspLU11I 1771 ACATGT A^CATGT BssSI 3969 CTCGTG CACGAG(-5/-1) Bsu36I 6800 CCTCAGG CC^T[ACGT]AGG ClaI 3666 ATCGAT AT^CGAT CpoI 3741 CGGTCCG CG^G[AT]CCG Eco81I 6800 CCTCAGG CC^T[ACGT]AGG FspI 3175 TGCGCA TGC^GCA HpaI 5557 GTTAAC GTT^AAC KpnI 6737 GGTACC GGTAC^C MfeI 3533 CAATTG C^AATTG MroI 3220 TCCGGA T^CCGGA MunI 3533 CAATTG C^AATTG PshAI 3434 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3099 ATTAAT AT^TAAT RsrII 3741 CGGTCCG CG^G[AT]CCG SacI 4823 GAGCTC GAGCT^C SalI 4250 GTCGAC G^TCGAC SfiI 2630 GGCCAACGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2206 CCCGGG CCC^GGG SpeI 2885 ACTAGT A^CTAGT SphI 3904 GCATGC GCATG^C XmaCI 2206 CCCGGG C^CCGGG XmaI 2206 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AatII 82 GACGTC GACGT^C 2519 GACGTC AccI 3033 GTCTAC GT^[AC][GT]AC 4250 GTCGAC AccII 3813 CGCG CG^CG 3994 CGCG AcyI 82 GACGTC G[GA]^CG[CT]C 2519 GACGTC AhdI 5782 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7105 GACCAACCGTC AspEI 5782 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7105 GACCAACCGTC AvrII 593 CCTAGG C^CTAGG 7158 CCTAGG BglII 653 AGATCT A^GATCT 6807 AGATCT BlnI 593 CCTAGG C^CTAGG 7158 CCTAGG BlpI 2055 GCTAAGC GC^T[ACGT]AGC 2178 GCTCAGC Bpu1102I 2055 GCTAAGC GC^T[ACGT]AGC 2178 GCTCAGC BsaBI 1640 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1808 GATCCCCATC BsaHI 82 GACGTC G[GA]^CG[CT]C 2519 GACGTC Bsp1407I 4486 TGTACA T^GTACA 5076 TGTACA BspHI 1060 TCATGA T^CATGA 3817 TCATGA BsrBI 3758 CCGCTC CCGCTC(-3/-3) 1602 GAGCGG BsrGI 4486 TGTACA T^GTACA 5076 TGTACA BstBI 1129 TTCGAA TT^CGAA 1359 TTCGAA BstEII 2486 GGTCACC G^GT[ACGT]ACC 7187 GGTCACC BstPI 2486 GGTCACC G^GT[ACGT]ACC 7187 GGTCACC BstUI 3813 CGCG CG^CG 3994 CGCG CelII 2055 GCTAAGC GC^T[ACGT]AGC 2178 GCTCAGC DraI 5839 TTTAAA TTT^AAA 5864 TTTAAA Eam1105I 5782 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 7105 GACCAACCGTC EcoO65I 2486 GGTCACC G^GT[ACGT]ACC 7187 GGTCACC HgaI 3814 GCGTC GACGC(5/10) 4501 GCGTC Hin1I 82 GACGTC G[GA]^CG[CT]C 2519 GACGTC MamI 1640 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1808 GATCCCCATC MvnI 3813 CGCG CG^CG 3994 CGCG NcoI 1000 CCATGG C^CATGG 5190 CCATGG NspV 1129 TTCGAA TT^CGAA 1359 TTCGAA RcaI 1060 TCATGA T^CATGA 3817 TCATGA ScaI 384 AGTACT AGT^ACT 4862 AGTACT SfuI 1129 TTCGAA TT^CGAA 1359 TTCGAA SspBI 4486 TGTACA T^GTACA 5076 TGTACA StuI 2808 AGGCCT AGG^CCT 4734 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI Asp700I AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoII XmnI