##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII BbeI
BbrPI BbvCI BcgI BglI BsaHI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I FseI FspI HaeII Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NspV PinAI PmaCI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I SwaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AclI 5162 AACGTT AA^CGTT
AocI 78 CCTGAGG CC^T[ACGT]AGG
AspI 1816 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 174 CCTAGG C^CTAGG
BalI 358 TGGCCA TGG^CCA
BanI 5936 GGTGCC G^G[CT][GA]CC
BlnI 174 CCTAGG C^CTAGG
BsgI 2017 GTGCAG GTGCAG(16/14)
BsrDI 4063 GCAATG GCAATG(2/0)
Bst1107I 3479 GTATAC GTA^TAC
BstAPI 538 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3479 GTATAC GTA^TAC
Bsu36I 78 CCTGAGG CC^T[ACGT]AGG
CfoI 894 GCGC GCG^C
Eco81I 78 CCTGAGG CC^T[ACGT]AGG
EcoNI 1593 CCTATTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2178 GAATTC G^AATTC
EcoT22I 545 ATGCAT ATGCA^T
HgaI 401 GCGTC GACGC(5/10)
HhaI 894 GCGC GCG^C
HinP1I 894 GCGC G^CGC
MfeI 971 CAATTG C^AATTG
MluNI 358 TGGCCA TGG^CCA
MscI 358 TGGCCA TGG^CCA
MunI 971 CAATTG C^AATTG
NsiI 545 ATGCAT ATGCA^T
PacI 7533 TTAATTAA TTAAT^TAA
PflFI 1816 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 5918 GTTTAAAC GTTT^AAAC
Psp1406I 5162 AACGTT AA^CGTT
ScaI 6665 AGTACT AGT^ACT
SnaBI 3219 TACGTA TAC^GTA
SphI 5891 GCATGC GCATG^C
Tth111I 1816 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3479 GTATAC GT^[AC][GT]AC
5155 GTAGAC
AccII 36 CGCG CG^CG
400 CGCG
AvaI 4925 CTCGAG C^[CT]CG[GA]G
4937 CTCGAG
BamHI 5362 GGATCC G^GATCC
5399 GGATCC
BciVI 3921 GGATAC GTATCC(6/5)
4468 GGATAC
BcnI 3 CCGGG CC^[GC]GG
708 CCGGG
BsaAI 3219 TACGTA [CT]AC^GT[GA]
3233 TACGTG
BsaI 6523 GAGACC GGTCTC(1/5)
3305 GGTCTC
BsaWI 2108 TCCGGT [AT]^CCGG[AT]
3716 TCCGGT
BsmFI 3773 GGGAC GGGAC(10/14)
4639 GTCCC
BsoBI 4925 CTCGAG C^[CT]CG[GA]G
4937 CTCGAG
BspLU11I 2382 ACATGT A^CATGT
6548 ACATGT
BspMI 3483 ACCTGC ACCTGC(4/8)
6396 ACCTGC
BstUI 36 CGCG CG^CG
400 CGCG
DrdI 394 GACCGTCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
967 GACCCAATTGTC
DsaI 41 CCATGG C^C[GA][CT]GG
4772 CCATGG
EaeI 96 TGGCCG [CT]^GGCC[GA]
358 TGGCCA
FauI 666 CCCGC CCCGC(4/6)
47 GCGGG
HpaI 6830 GTTAAC GTT^AAC
7176 GTTAAC
MvnI 36 CGCG CG^CG
400 CGCG
NciI 3 CCGGG CC^[GC]GG
708 CCGGG
NcoI 41 CCATGG C^CATGG
4772 CCATGG
PaeR7I 4925 CTCGAG C^TCGAG
4937 CTCGAG
PflMI 6481 CCATTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6598 CCATCTACTGG
SacI 1620 GAGCTC GAGCT^C
4675 GAGCTC
Van91I 6481 CCATTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6598 CCATCTACTGG
XbaI 2835 TCTAGA T^CTAGA
6079 TCTAGA
XhoI 4925 CTCGAG C^TCGAG
4937 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BanII
BbsI BbvI BclI BfaI BfrI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmI
Bsp1286I Bsp1407I BspHI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII DraIII EarI EcoO109I
EcoRII EcoRV EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI PstI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI