##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AseI        AsnI        Asp700I     AspI        AvrII       
BamHI       BfrI        BglII       BlnI        BsaBI       
BsaWI       BseAI       BsiWI       BspEI       BspHI       
CpoI        DraI        EcoT22I     FseI        HincII      
HindII      HpaI        MamI        MfeI        MluI        
MroI        MseI        MunI        NdeI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SgrAI       SnaBI       SpeI        
SspI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AcsI         4515  GAATTC               [GA]^AATT[CT]
ApoI         4515  GAATTC               [GA]^AATT[CT]
AscI         3194  GGCGCGCC             GG^CGCGCC
BclI         5593  TGATCA               T^GATCA
BspDI        3840  ATCGAT               AT^CGAT
BspLU11I     1546  ACATGT               A^CATGT
BsrDI        4115  CATTGC               GCAATG(2/0)
Bst1107I     3699  GTATAC               GTA^TAC
BstBI        3036  TTCGAA               TT^CGAA
BstZ17I      3699  GTATAC               GTA^TAC
ClaI         3840  ATCGAT               AT^CGAT
DrdI         1740  GACGTGCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        4515  GAATTC               G^AATTC
EcoRV        4079  GATATC               GAT^ATC
FbaI         5593  TGATCA               T^GATCA
HindIII      4289  AAGCTT               A^AGCTT
NheI         5024  GCTAGC               G^CTAGC
NotI         6357  GCGGCCGC             GC^GGCCGC
NspV         3036  TTCGAA               TT^CGAA
PshAI        3887  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         4093  AGTACT               AGT^ACT
SfiI         6172  GGCCCGGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI         3036  TTCGAA               TT^CGAA
Tsp509I      4516  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

Acc65I        188  GGTACC               G^GTACC
             2855  GGTACC              
Alw44I        383  GTGCAC               G^TGCAC
              878  GTGCAC              
ApaLI         383  GTGCAC               G^TGCAC
              878  GTGCAC              
Asp718I       188  GGTACC               G^GTACC
             2855  GGTACC              
BciVI         257  GGATAC               GTATCC(6/5)
              597  GGATAC              
BsmBI         897  GAGACG               CGTCTC(1/5)
             6096  GAGACG              
BsmI         3086  GAATGC               GAATGC(1/-1)
             4983  GCATTC              
Bsp1407I      197  TGTACA               T^GTACA
              307  TGTACA              
BsrGI         197  TGTACA               T^GTACA
              307  TGTACA              
BstYI        1798  AGATCC               [GA]^GATC[CT]
             5574  GGATCT              
KpnI          188  GGTACC               GGTAC^C
             2855  GGTACC              
KspI         2046  CCGCGG               CCGC^GG
             5612  CCGCGG              
MflI         1798  AGATCC               [GA]^GATC[CT]
             5574  GGATCT              
NaeI          478  GCCGGC               GCC^GGC
             6317  GCCGGC              
NgoMIV        478  GCCGGC               G^CCGGC
             6317  GCCGGC              
SacII        2046  CCGCGG               CCGC^GG
             5612  CCGCGG              
SapI         1484  GCTCTTC              GCTCTTC(1/4)
             3830  GCTCTTC             
SexAI         496  ACCTGGT              A^CC[AT]GGT
             3961  ACCAGGT             
SspBI         197  TGTACA               T^GTACA
              307  TGTACA              
XhoII        1798  AGATCC               [GA]^GATC[CT]
             5574  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  156 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AhdI        AluI        AlwI        AlwNI       
AocI        Aor51HI     ApaI        AspEI       AspHI       
AvaI        AvaII       AviII       BalI        BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssHII      BssKI       
BssSI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      SbfI        ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        SphI        Sse8387I    
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI