################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AccIII AclI AflII AgeI AseI AsnI Asp700I AspI AvrII BamHI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BspEI BspHI CpoI DraI EcoT22I FseI HincII HindII HpaI MamI MfeI MluI MroI MseI MunI NdeI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SgrAI SnaBI SpeI SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AcsI 4515 GAATTC [GA]^AATT[CT] ApoI 4515 GAATTC [GA]^AATT[CT] AscI 3194 GGCGCGCC GG^CGCGCC BclI 5593 TGATCA T^GATCA BspDI 3840 ATCGAT AT^CGAT BspLU11I 1546 ACATGT A^CATGT BsrDI 4115 CATTGC GCAATG(2/0) Bst1107I 3699 GTATAC GTA^TAC BstBI 3036 TTCGAA TT^CGAA BstZ17I 3699 GTATAC GTA^TAC ClaI 3840 ATCGAT AT^CGAT DrdI 1740 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 4515 GAATTC G^AATTC EcoRV 4079 GATATC GAT^ATC FbaI 5593 TGATCA T^GATCA HindIII 4289 AAGCTT A^AGCTT NheI 5024 GCTAGC G^CTAGC NotI 6357 GCGGCCGC GC^GGCCGC NspV 3036 TTCGAA TT^CGAA PshAI 3887 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 4093 AGTACT AGT^ACT SfiI 6172 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 3036 TTCGAA TT^CGAA Tsp509I 4516 AATT ^AATT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### Acc65I 188 GGTACC G^GTACC 2855 GGTACC Alw44I 383 GTGCAC G^TGCAC 878 GTGCAC ApaLI 383 GTGCAC G^TGCAC 878 GTGCAC Asp718I 188 GGTACC G^GTACC 2855 GGTACC BciVI 257 GGATAC GTATCC(6/5) 597 GGATAC BsmBI 897 GAGACG CGTCTC(1/5) 6096 GAGACG BsmI 3086 GAATGC GAATGC(1/-1) 4983 GCATTC Bsp1407I 197 TGTACA T^GTACA 307 TGTACA BsrGI 197 TGTACA T^GTACA 307 TGTACA BstYI 1798 AGATCC [GA]^GATC[CT] 5574 GGATCT KpnI 188 GGTACC GGTAC^C 2855 GGTACC KspI 2046 CCGCGG CCGC^GG 5612 CCGCGG MflI 1798 AGATCC [GA]^GATC[CT] 5574 GGATCT NaeI 478 GCCGGC GCC^GGC 6317 GCCGGC NgoMIV 478 GCCGGC G^CCGGC 6317 GCCGGC SacII 2046 CCGCGG CCGC^GG 5612 CCGCGG SapI 1484 GCTCTTC GCTCTTC(1/4) 3830 GCTCTTC SexAI 496 ACCTGGT A^CC[AT]GGT 3961 ACCAGGT SspBI 197 TGTACA T^GTACA 307 TGTACA XhoII 1798 AGATCC [GA]^GATC[CT] 5574 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 156 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AhdI AluI AlwI AlwNI AocI Aor51HI ApaI AspEI AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfoI SmaI SmlI SphI Sse8387I StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmaCI XmaI