################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AgeI AseI AsnI AviII AvrII BlnI BsiWI BspDI BsrFI Cfr10I ClaI DraIII EagI EclXI Eco52I EcoRV FseI FspI MfeI MluI MunI NaeI NdeI NgoMIV NotI PacI PaeR7I PinAI PmeI PshBI PvuI SalI SapI SbfI ScaI SfiI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 4446 GGTACC G^GTACC AclI 5489 AACGTT AA^CGTT AflII 6132 CTTAAG C^TTAAG AocI 4017 CCTCAGG CC^T[ACGT]AGG Aor51HI 342 AGCGCT AGC^GCT ApaI 984 GGGCCC GGGCC^C AscI 136 GGCGCGCC GG^CGCGCC Asp700I 5272 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 4446 GGTACC G^GTACC AspI 4070 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 3173 CACGTG CAC^GTG BbvCI 2252 CCTCAGC CCTCAGC(-5/-2) BciVI 3424 GGATAC GTATCC(6/5) BclI 1773 TGATCA T^GATCA BfrI 6132 CTTAAG C^TTAAG BlpI 2618 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2618 GCTGAGC GC^T[ACGT]AGC BsiEI 887 CGACCG CG[GA][CT]^CG Bsp1407I 5482 TGTACA T^GTACA BspLU11I 4752 ACATGT A^CATGT BsrGI 5482 TGTACA T^GTACA BssHII 137 GCGCGC G^CGCGC BstBI 5361 TTCGAA TT^CGAA Bsu36I 4017 CCTCAGG CC^T[ACGT]AGG CelII 2618 GCTGAGC GC^T[ACGT]AGC CpoI 93 CGGACCG CG^G[AT]CCG Eco47III 342 AGCGCT AGC^GCT Eco81I 4017 CCTCAGG CC^T[ACGT]AGG FbaI 1773 TGATCA T^GATCA HincII 5458 GTTAAC GT[CT]^[GA]AC HindII 5458 GTTAAC GT[CT]^[GA]AC HindIII 255 AAGCTT A^AGCTT HpaI 5458 GTTAAC GTT^AAC KpnI 4446 GGTACC GGTAC^C NheI 3526 GCTAGC G^CTAGC NruI 3282 TCGCGA TCG^CGA NspV 5361 TTCGAA TT^CGAA PflFI 4070 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3173 CACGTG CAC^GTG PmlI 3173 CACGTG CAC^GTG Psp1406I 5489 AACGTT AA^CGTT RsrII 93 CGGACCG CG^G[AT]CCG SfuI 5361 TTCGAA TT^CGAA SpeI 1409 ACTAGT A^CTAGT SspBI 5482 TGTACA T^GTACA SspI 5543 AATATT AAT^ATT Tth111I 4070 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 5272 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 2073 TCCGGA T^CCGGA 6100 TCCGGA AhdI 2019 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6091 GACAAAGTGTC AspEI 2019 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6091 GACAAAGTGTC BbeI 75 GGCGCC GGCGC^C 1087 GGCGCC BglII 3459 AGATCT A^GATCT 5238 AGATCT BsaBI 1774 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2605 GATCGTGATC BseAI 2073 TCCGGA T^CCGGA 6100 TCCGGA BspEI 2073 TCCGGA T^CCGGA 6100 TCCGGA BspHI 1755 TCATGA T^CATGA 5190 TCATGA BssSI 333 CACGAG CACGAG(-5/-1) 2204 CTCGTG Bst1107I 2691 GTATAC GTA^TAC 5351 GTATAC BstEII 2581 GGTGACC G^GT[ACGT]ACC 4591 GGTGACC BstPI 2581 GGTGACC G^GT[ACGT]ACC 4591 GGTGACC BstZ17I 2691 GTATAC GTA^TAC 5351 GTATAC DrdI 2305 GACGGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4649 GACATCGGCGTC Eam1105I 2019 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6091 GACAAAGTGTC EcoO65I 2581 GGTGACC G^GT[ACGT]ACC 4591 GGTGACC KasI 75 GGCGCC G^GCGCC 1087 GGCGCC KspI 80 CCGCGG CCGC^GG 3956 CCGCGG MamI 1774 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2605 GATCGTGATC MroI 2073 TCCGGA T^CCGGA 6100 TCCGGA NarI 75 GGCGCC GG^CGCC 1087 GGCGCC PflMI 655 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 988 CCAGATGCTGG RcaI 1755 TCATGA T^CATGA 5190 TCATGA SacII 80 CCGCGG CCGC^GG 3956 CCGCGG SexAI 2913 ACCTGGT A^CC[AT]GGT 4578 ACCTGGT SfoI 75 GGCGCC GGC^GCC 1087 GGCGCC SmaI 2104 CCCGGG CCC^GGG 6193 CCCGGG SphI 4440 GCATGC GCATG^C 4692 GCATGC Van91I 655 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 988 CCAGATGCTGG XmaCI 2104 CCCGGG C^CCGGG 6193 CCCGGG XmaI 2104 CCCGGG C^CCGGG 6193 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### AatII AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII