##################################
# NO CUT ENZYMES (   41 enzymes) #
##################################

AgeI        AseI        AsnI        AviII       AvrII       
BlnI        BsiWI       BspDI       BsrFI       Cfr10I      
ClaI        DraIII      EagI        EclXI       Eco52I      
EcoRV       FseI        FspI        MfeI        MluI        
MunI        NaeI        NdeI        NgoMIV      NotI        
PacI        PaeR7I      PinAI       PmeI        PshBI       
PvuI        SalI        SapI        SbfI        ScaI        
SfiI        SgrAI       SnaBI       Sse8387I    SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       4446  GGTACC               G^GTACC
AclI         5489  AACGTT               AA^CGTT
AflII        6132  CTTAAG               C^TTAAG
AocI         4017  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       342  AGCGCT               AGC^GCT
ApaI          984  GGGCCC               GGGCC^C
AscI          136  GGCGCGCC             GG^CGCGCC
Asp700I      5272  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      4446  GGTACC               G^GTACC
AspI         4070  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        3173  CACGTG               CAC^GTG
BbvCI        2252  CCTCAGC              CCTCAGC(-5/-2)
BciVI        3424  GGATAC               GTATCC(6/5)
BclI         1773  TGATCA               T^GATCA
BfrI         6132  CTTAAG               C^TTAAG
BlpI         2618  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2618  GCTGAGC              GC^T[ACGT]AGC
BsiEI         887  CGACCG               CG[GA][CT]^CG
Bsp1407I     5482  TGTACA               T^GTACA
BspLU11I     4752  ACATGT               A^CATGT
BsrGI        5482  TGTACA               T^GTACA
BssHII        137  GCGCGC               G^CGCGC
BstBI        5361  TTCGAA               TT^CGAA
Bsu36I       4017  CCTCAGG              CC^T[ACGT]AGG
CelII        2618  GCTGAGC              GC^T[ACGT]AGC
CpoI           93  CGGACCG              CG^G[AT]CCG
Eco47III      342  AGCGCT               AGC^GCT
Eco81I       4017  CCTCAGG              CC^T[ACGT]AGG
FbaI         1773  TGATCA               T^GATCA
HincII       5458  GTTAAC               GT[CT]^[GA]AC
HindII       5458  GTTAAC               GT[CT]^[GA]AC
HindIII       255  AAGCTT               A^AGCTT
HpaI         5458  GTTAAC               GTT^AAC
KpnI         4446  GGTACC               GGTAC^C
NheI         3526  GCTAGC               G^CTAGC
NruI         3282  TCGCGA               TCG^CGA
NspV         5361  TTCGAA               TT^CGAA
PflFI        4070  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3173  CACGTG               CAC^GTG
PmlI         3173  CACGTG               CAC^GTG
Psp1406I     5489  AACGTT               AA^CGTT
RsrII          93  CGGACCG              CG^G[AT]CCG
SfuI         5361  TTCGAA               TT^CGAA
SpeI         1409  ACTAGT               A^CTAGT
SspBI        5482  TGTACA               T^GTACA
SspI         5543  AATATT               AAT^ATT
Tth111I      4070  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         5272  GAAAACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII       2073  TCCGGA               T^CCGGA
             6100  TCCGGA              
AhdI         2019  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6091  GACAAAGTGTC         
AspEI        2019  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6091  GACAAAGTGTC         
BbeI           75  GGCGCC               GGCGC^C
             1087  GGCGCC              
BglII        3459  AGATCT               A^GATCT
             5238  AGATCT              
BsaBI        1774  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2605  GATCGTGATC          
BseAI        2073  TCCGGA               T^CCGGA
             6100  TCCGGA              
BspEI        2073  TCCGGA               T^CCGGA
             6100  TCCGGA              
BspHI        1755  TCATGA               T^CATGA
             5190  TCATGA              
BssSI         333  CACGAG               CACGAG(-5/-1)
             2204  CTCGTG              
Bst1107I     2691  GTATAC               GTA^TAC
             5351  GTATAC              
BstEII       2581  GGTGACC              G^GT[ACGT]ACC
             4591  GGTGACC             
BstPI        2581  GGTGACC              G^GT[ACGT]ACC
             4591  GGTGACC             
BstZ17I      2691  GTATAC               GTA^TAC
             5351  GTATAC              
DrdI         2305  GACGGGCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4649  GACATCGGCGTC        
Eam1105I     2019  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             6091  GACAAAGTGTC         
EcoO65I      2581  GGTGACC              G^GT[ACGT]ACC
             4591  GGTGACC             
KasI           75  GGCGCC               G^GCGCC
             1087  GGCGCC              
KspI           80  CCGCGG               CCGC^GG
             3956  CCGCGG              
MamI         1774  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2605  GATCGTGATC          
MroI         2073  TCCGGA               T^CCGGA
             6100  TCCGGA              
NarI           75  GGCGCC               GG^CGCC
             1087  GGCGCC              
PflMI         655  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              988  CCAGATGCTGG         
RcaI         1755  TCATGA               T^CATGA
             5190  TCATGA              
SacII          80  CCGCGG               CCGC^GG
             3956  CCGCGG              
SexAI        2913  ACCTGGT              A^CC[AT]GGT
             4578  ACCTGGT             
SfoI           75  GGCGCC               GGC^GCC
             1087  GGCGCC              
SmaI         2104  CCCGGG               CCC^GGG
             6193  CCCGGG              
SphI         4440  GCATGC               GCATG^C
             4692  GCATGC              
Van91I        655  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              988  CCAGATGCTGG         
XmaCI        2104  CCCGGG               C^CCGGG
             6193  CCCGGG              
XmaI         2104  CCCGGG               C^CCGGG
             6193  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AatII       AccI        AccII       AciI        AcsI        
AcyI        AfaI        AflIII      AluI        Alw44I      
AlwI        AlwNI       ApaLI       ApoI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbsI        BbvI        BcgI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     EcoT22I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PpuMI       PshAI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI        
XcmI        XhoII