##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AgeI Aor51HI AscI AviII
AvrII BamHI BbeI BbrPI BciVI
BclI BlnI BseAI BsiWI BspDI
BspEI BssHII BstBI BstEII BstPI
ClaI CpoI Eco47III EcoO65I EcoT22I
FbaI FseI FspI KasI MluI
MroI NaeI NarI NgoMIV NheI
NotI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 362 GACGTC GACGT^C
Acc65I 3283 GGTACC G^GTACC
AccI 6046 GTATAC GT^[AC][GT]AC
AclI 6393 AACGTT AA^CGTT
AhdI 2235 GACGGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2453 CCTGAGG CC^T[ACGT]AGG
Asp718I 3283 GGTACC G^GTACC
AspEI 2235 GACGGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1589 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 3499 GCAGGCAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2049 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 887 CACGTA [CT]AC^GT[GA]
BsaBI 5324 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 64 ACCGGA [AT]^CCGG[AT]
BsiEI 391 CGGCCG CG[GA][CT]^CG
BsmBI 2100 CGTCTC CGTCTC(1/5)
BspLU11I 5873 ACATGT A^CATGT
BsrBI 4481 GAGCGG CCGCTC(-3/-3)
BsrDI 592 GCAATG GCAATG(2/0)
BssSI 2190 CACGAG CACGAG(-5/-1)
Bst1107I 6046 GTATAC GTA^TAC
BstXI 625 CCAAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6046 GTATAC GTA^TAC
Bsu36I 2453 CCTGAGG CC^T[ACGT]AGG
EagI 391 CGGCCG C^GGCCG
Eam1105I 2235 GACGGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 391 CGGCCG C^GGCCG
Eco52I 391 CGGCCG C^GGCCG
Eco81I 2453 CCTGAGG CC^T[ACGT]AGG
EcoRI 2835 GAATTC G^AATTC
EcoRV 3198 GATATC GAT^ATC
HpaI 6039 GTTAAC GTT^AAC
KpnI 3283 GGTACC GGTAC^C
KspI 2488 CCGCGG CCGC^GG
MamI 5324 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 6413 CAATTG C^AATTG
MunI 6413 CAATTG C^AATTG
NdeI 6561 CATATG CA^TATG
NruI 3378 TCGCGA TCG^CGA
PaeR7I 3310 CTCGAG C^TCGAG
PflFI 1589 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 6393 AACGTT AA^CGTT
SacI 1554 GAGCTC GAGCT^C
SacII 2488 CCGCGG CCGC^GG
ScaI 5859 AGTACT AGT^ACT
SmaI 683 CCCGGG CCC^GGG
SphI 1000 GCATGC GCATG^C
Tth111I 1589 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2870 TCTAGA T^CTAGA
XhoI 3310 CTCGAG C^TCGAG
XmaCI 683 CCCGGG C^CCGGG
XmaI 683 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Alw44I 915 GTGCAC G^TGCAC
1353 GTGCAC
ApaI 3638 GGGCCC GGGCC^C
4154 GGGCCC
ApaLI 915 GTGCAC G^TGCAC
1353 GTGCAC
BbvCI 220 CCTCAGC CCTCAGC(-5/-2)
1373 CCTCAGC
BlpI 1004 GCTGAGC GC^T[ACGT]AGC
4491 GCTGAGC
Bpu1102I 1004 GCTGAGC GC^T[ACGT]AGC
4491 GCTGAGC
BsrFI 940 GCCGGT [GA]^CCGG[CT]
3851 GCCGGT
CelII 1004 GCTGAGC GC^T[ACGT]AGC
4491 GCTGAGC
Cfr10I 940 GCCGGT [GA]^CCGG[CT]
3851 GCCGGT
DraI 6161 TTTAAA TTT^AAA
6312 TTTAAA
DrdI 179 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4610 GACAAACACGTC
HindIII 1281 AAGCTT A^AGCTT
5989 AAGCTT
NcoI 378 CCATGG C^CATGG
1927 CCATGG
SapI 2459 GAAGAGC GCTCTTC(1/4)
4342 GCTCTTC
SspI 5799 AATATT AAT^ATT
6500 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI