##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaI
BsaWI BseAI BseRI BsiWI BsmBI
BspDI BspEI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I FseI
FspI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NlaIV NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacI SacII
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SmlI SpeI Sse8387I
StuI SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1992 GACGTC GACGT^C
AccI 2665 GTCGAC GT^[AC][GT]AC
AcyI 1992 GACGTC G[GA]^CG[CT]C
Aor51HI 2017 AGCGCT AGC^GCT
AspHI 6627 GTGCTC G[AT]GC[AT]^C
AspI 2710 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5533 CTCGGG C^[CT]CG[GA]G
BanII 493 GGGCTC G[GA]GC[CT]^C
BcnI 2920 CCGGG CC^[GC]GG
BlpI 2985 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2985 GCTCAGC GC^T[ACGT]AGC
BsaHI 1992 GACGTC G[GA]^CG[CT]C
BsiEI 515 CGATCG CG[GA][CT]^CG
BsiHKAI 6627 GTGCTC G[AT]GC[AT]^C
BsmI 2642 GCATTC GAATGC(1/-1)
BsoBI 5533 CTCGGG C^[CT]CG[GA]G
BspMI 1967 GCAGGT ACCTGC(4/8)
BsrFI 2500 ACCGGC [GA]^CCGG[CT]
BssSI 948 CACGAG CACGAG(-5/-1)
CelII 2985 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2500 ACCGGC [GA]^CCGG[CT]
Eco47III 2017 AGCGCT AGC^GCT
EcoRV 150 GATATC GAT^ATC
FauI 1733 GCGGG CCCGC(4/6)
Hin1I 1992 GACGTC G[GA]^CG[CT]C
HindIII 3213 AAGCTT A^AGCTT
NciI 2920 CCGGG CC^[GC]GG
PacI 357 TTAATTAA TTAAT^TAA
PflFI 2710 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6655 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 515 CGATCG CGAT^CG
SalI 2665 GTCGAC G^TCGAC
ScaI 2400 AGTACT AGT^ACT
SexAI 3097 ACCAGGT A^CC[AT]GGT
SnaBI 1471 TACGTA TAC^GTA
SphI 4488 GCATGC GCATG^C
Tth111I 2710 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6655 CCATCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3098 CCAGGTTTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AocI 2704 CCTTAGG CC^T[ACGT]AGG
4078 CCTGAGG
BalI 845 TGGCCA TGG^CCA
6652 TGGCCA
BciVI 3168 GGATAC GTATCC(6/5)
4017 GGATAC
BglII 828 AGATCT A^GATCT
6307 AGATCT
BpmI 2482 CTCCAG CTGGAG(16/14)
5614 CTGGAG
BsaAI 1471 TACGTA [CT]AC^GT[GA]
2431 TACGTG
Bsp1407I 4479 TGTACA T^GTACA
6196 TGTACA
BsrGI 4479 TGTACA T^GTACA
6196 TGTACA
BstXI 3029 CCAACAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4663 CCAAGGTTGTGG
BstYI 828 AGATCT [GA]^GATC[CT]
6307 AGATCT
Bsu36I 2704 CCTTAGG CC^T[ACGT]AGG
4078 CCTGAGG
CfoI 982 GCGC GCG^C
2018 GCGC
DraII 1969 AGGTCCT [GA]G^G[ACGT]CC[CT]
2903 AGGACCT
DraIII 1135 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5380 CACCTTGTG
DsaI 1076 CCATGG C^C[GA][CT]GG
1784 CCATGG
Eco81I 2704 CCTTAGG CC^T[ACGT]AGG
4078 CCTGAGG
EcoO109I 1969 AGGTCCT [GA]G^G[ACGT]CC[CT]
2903 AGGACCT
HaeII 981 GGCGCT [GA]GCGC^[CT]
2017 AGCGCT
HapII 2501 CCGG C^CGG
2920 CCGG
HgaI 2215 GACGC GACGC(5/10)
1347 GCGTC
HhaI 982 GCGC GCG^C
2018 GCGC
HinP1I 982 GCGC G^CGC
2018 GCGC
HpaI 119 GTTAAC GTT^AAC
1797 GTTAAC
HpaII 2501 CCGG C^CGG
2920 CCGG
MflI 828 AGATCT [GA]^GATC[CT]
6307 AGATCT
MluNI 845 TGGCCA TGG^CCA
6652 TGGCCA
MscI 845 TGGCCA TGG^CCA
6652 TGGCCA
MspI 2501 CCGG C^CGG
2920 CCGG
NcoI 1076 CCATGG C^CATGG
1784 CCATGG
NdeI 1953 CATATG CA^TATG
3338 CATATG
PpuMI 1969 AGGTCCT [GA]G^G[AT]CC[CT]
2903 AGGACCT
SspBI 4479 TGTACA T^GTACA
6196 TGTACA
XbaI 1283 TCTAGA T^CTAGA
4795 TCTAGA
XhoII 828 AGATCT [GA]^GATC[CT]
6307 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BclI
BfaI BmyI BpuAI BsaBI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspLU11I BsrDI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EaeI
EarI EcoRI EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MnlI MseI MslI
MspA1I MunI MvaI MwoI NdeII
NlaIII NsiI NspI PleI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI