################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AccIII AflII AgeI AocI AscI AvrII BbvCI BcgI BciVI BfrI BlnI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrDI Bst1107I BstBI BstXI BstZ17I Bsu36I ClaI CpoI EagI EclXI Eco52I Eco81I EcoRV FseI HindIII HpaI MluI MroI NotI NruI NspV PacI PaeR7I PinAI PmeI PvuI RcaI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 965 GACGTC GACGT^C Acc65I 3466 GGTACC G^GTACC AclI 2627 AACGTT AA^CGTT Aor51HI 1033 AGCGCT AGC^GCT Asp718I 3466 GGTACC G^GTACC AviII 2549 TGCGCA TGC^GCA BamHI 1884 GGATCC G^GATCC BbrPI 262 CACGTG CAC^GTG BclI 4452 TGATCA T^GATCA BglI 662 GCCCTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1177 AGATCT A^GATCT BsaBI 4383 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 305 CTCCTC GAGGAG(10/8) BsiEI 1019 CGACCG CG[GA][CT]^CG Bsp1407I 3958 TGTACA T^GTACA BsrGI 3958 TGTACA T^GTACA BstAPI 539 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2095 GGTCACC G^GT[ACGT]ACC BstPI 2095 GGTCACC G^GT[ACGT]ACC DrdI 4765 GACAAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1033 AGCGCT AGC^GCT EcoO65I 2095 GGTCACC G^GT[ACGT]ACC FbaI 4452 TGATCA T^GATCA FspI 2549 TGCGCA TGC^GCA KpnI 3466 GGTACC GGTAC^C MamI 4383 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4873 CAATTG C^AATTG MunI 4873 CAATTG C^AATTG NheI 786 GCTAGC G^CTAGC PmaCI 262 CACGTG CAC^GTG PmlI 262 CACGTG CAC^GTG PshAI 4076 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2627 AACGTT AA^CGTT SexAI 2862 ACCTGGT A^CC[AT]GGT SpeI 5514 ACTAGT A^CTAGT SphI 4415 GCATGC GCATG^C SspBI 3958 TGTACA T^GTACA XcmI 772 CCACGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AhdI 1086 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3701 GACCAAAAGTC Alw44I 678 GTGCAC G^TGCAC 2131 GTGCAC ApaI 815 GGGCCC GGGCC^C 5436 GGGCCC ApaLI 678 GTGCAC G^TGCAC 2131 GTGCAC AseI 2378 ATTAAT AT^TAAT 5609 ATTAAT AsnI 2378 ATTAAT AT^TAAT 5609 ATTAAT AspEI 1086 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3701 GACCAAAAGTC AspI 1603 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2267 GACATAGTC BalI 973 TGGCCA TGG^CCA 1559 TGGCCA BlpI 141 GCTGAGC GC^T[ACGT]AGC 2809 GCTGAGC Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC 2809 GCTGAGC BsrBI 60 CCGCTC CCGCTC(-3/-3) 330 CCGCTC BsrFI 574 GCCGGC [GA]^CCGG[CT] 853 GCCGGC BssHII 49 GCGCGC G^CGCGC 942 GCGCGC BssSI 543 CTCGTG CACGAG(-5/-1) 615 CTCGTG CelII 141 GCTGAGC GC^T[ACGT]AGC 2809 GCTGAGC Cfr10I 574 GCCGGC [GA]^CCGG[CT] 853 GCCGGC DraIII 1110 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 2134 CACGTCGTG Eam1105I 1086 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3701 GACCAAAAGTC KspI 319 CCGCGG CCGC^GG 884 CCGCGG MluNI 973 TGGCCA TGG^CCA 1559 TGGCCA MscI 973 TGGCCA TGG^CCA 1559 TGGCCA NaeI 574 GCCGGC GCC^GGC 853 GCCGGC NcoI 1799 CCATGG C^CATGG 4229 CCATGG NdeI 3434 CATATG CA^TATG 4426 CATATG NgoMIV 574 GCCGGC G^CCGGC 853 GCCGGC PflFI 1603 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2267 GACATAGTC PshBI 2378 ATTAAT AT^TAAT 5609 ATTAAT PvuII 1971 CAGCTG CAG^CTG 3290 CAGCTG SacI 612 GAGCTC GAGCT^C 1219 GAGCTC SacII 319 CCGCGG CCGC^GG 884 CCGCGG SapI 5918 GAAGAGC GCTCTTC(1/4) 332 GCTCTTC ScaI 595 AGTACT AGT^ACT 2794 AGTACT SmaI 460 CCCGGG CCC^GGG 650 CCCGGG StuI 1078 AGGCCT AGG^CCT 4261 AGGCCT Tth111I 1603 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2267 GACATAGTC XmaCI 460 CCCGGG C^CCGGG 650 CCCGGG XmaI 460 CCCGGG C^CCGGG 650 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI