##################################
# NO CUT ENZYMES (   57 enzymes) #
##################################

AccIII      AflII       AgeI        AocI        AscI        
AvrII       BbvCI       BcgI        BciVI       BfrI        
BlnI        BsaWI       BseAI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BsrDI       
Bst1107I    BstBI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        EagI        EclXI       Eco52I      
Eco81I      EcoRV       FseI        HindIII     HpaI        
MluI        MroI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PvuI        
RcaI        RsrII       SalI        SbfI        SfiI        
SfuI        SgrAI       SnaBI       Sse8387I    SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         965  GACGTC               GACGT^C
Acc65I       3466  GGTACC               G^GTACC
AclI         2627  AACGTT               AA^CGTT
Aor51HI      1033  AGCGCT               AGC^GCT
Asp718I      3466  GGTACC               G^GTACC
AviII        2549  TGCGCA               TGC^GCA
BamHI        1884  GGATCC               G^GATCC
BbrPI         262  CACGTG               CAC^GTG
BclI         4452  TGATCA               T^GATCA
BglI          662  GCCCTCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1177  AGATCT               A^GATCT
BsaBI        4383  GATAATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         305  CTCCTC               GAGGAG(10/8)
BsiEI        1019  CGACCG               CG[GA][CT]^CG
Bsp1407I     3958  TGTACA               T^GTACA
BsrGI        3958  TGTACA               T^GTACA
BstAPI        539  GCAGCTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2095  GGTCACC              G^GT[ACGT]ACC
BstPI        2095  GGTCACC              G^GT[ACGT]ACC
DrdI         4765  GACAAAATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1033  AGCGCT               AGC^GCT
EcoO65I      2095  GGTCACC              G^GT[ACGT]ACC
FbaI         4452  TGATCA               T^GATCA
FspI         2549  TGCGCA               TGC^GCA
KpnI         3466  GGTACC               GGTAC^C
MamI         4383  GATAATTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4873  CAATTG               C^AATTG
MunI         4873  CAATTG               C^AATTG
NheI          786  GCTAGC               G^CTAGC
PmaCI         262  CACGTG               CAC^GTG
PmlI          262  CACGTG               CAC^GTG
PshAI        4076  GACGACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2627  AACGTT               AA^CGTT
SexAI        2862  ACCTGGT              A^CC[AT]GGT
SpeI         5514  ACTAGT               A^CTAGT
SphI         4415  GCATGC               GCATG^C
SspBI        3958  TGTACA               T^GTACA
XcmI          772  CCACGCTGGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AhdI         1086  GACAAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3701  GACCAAAAGTC         
Alw44I        678  GTGCAC               G^TGCAC
             2131  GTGCAC              
ApaI          815  GGGCCC               GGGCC^C
             5436  GGGCCC              
ApaLI         678  GTGCAC               G^TGCAC
             2131  GTGCAC              
AseI         2378  ATTAAT               AT^TAAT
             5609  ATTAAT              
AsnI         2378  ATTAAT               AT^TAAT
             5609  ATTAAT              
AspEI        1086  GACAAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3701  GACCAAAAGTC         
AspI         1603  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2267  GACATAGTC           
BalI          973  TGGCCA               TGG^CCA
             1559  TGGCCA              
BlpI          141  GCTGAGC              GC^T[ACGT]AGC
             2809  GCTGAGC             
Bpu1102I      141  GCTGAGC              GC^T[ACGT]AGC
             2809  GCTGAGC             
BsrBI          60  CCGCTC               CCGCTC(-3/-3)
              330  CCGCTC              
BsrFI         574  GCCGGC               [GA]^CCGG[CT]
              853  GCCGGC              
BssHII         49  GCGCGC               G^CGCGC
              942  GCGCGC              
BssSI         543  CTCGTG               CACGAG(-5/-1)
              615  CTCGTG              
CelII         141  GCTGAGC              GC^T[ACGT]AGC
             2809  GCTGAGC             
Cfr10I        574  GCCGGC               [GA]^CCGG[CT]
              853  GCCGGC              
DraIII       1110  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2134  CACGTCGTG           
Eam1105I     1086  GACAAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3701  GACCAAAAGTC         
KspI          319  CCGCGG               CCGC^GG
              884  CCGCGG              
MluNI         973  TGGCCA               TGG^CCA
             1559  TGGCCA              
MscI          973  TGGCCA               TGG^CCA
             1559  TGGCCA              
NaeI          574  GCCGGC               GCC^GGC
              853  GCCGGC              
NcoI         1799  CCATGG               C^CATGG
             4229  CCATGG              
NdeI         3434  CATATG               CA^TATG
             4426  CATATG              
NgoMIV        574  GCCGGC               G^CCGGC
              853  GCCGGC              
PflFI        1603  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2267  GACATAGTC           
PshBI        2378  ATTAAT               AT^TAAT
             5609  ATTAAT              
PvuII        1971  CAGCTG               CAG^CTG
             3290  CAGCTG              
SacI          612  GAGCTC               GAGCT^C
             1219  GAGCTC              
SacII         319  CCGCGG               CCGC^GG
              884  CCGCGG              
SapI         5918  GAAGAGC              GCTCTTC(1/4)
              332  GCTCTTC             
ScaI          595  AGTACT               AGT^ACT
             2794  AGTACT              
SmaI          460  CCCGGG               CCC^GGG
              650  CCCGGG              
StuI         1078  AGGCCT               AGG^CCT
             4261  AGGCCT              
Tth111I      1603  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2267  GACATAGTC           
XmaCI         460  CCCGGG               C^CCGGG
              650  CCCGGG              
XmaI          460  CCCGGG               C^CCGGG
              650  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        Asp700I     AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspLU11I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRI       EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NarI        NciI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PflMI       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI