##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
ApoI AscI AseI AsnI AviII
BbrPI BciVI BfrI BsaBI BseAI
BsiWI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoRI EcoRV EcoT22I FseI
FspI HpaI MamI MfeI MluI
MroI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII ScaI SfiI SfuI
SnaBI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 442 GGTACC G^GTACC
AflIII 4322 ACATGT A^C[GA][CT]GT
AhdI 255 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1117 AGCGCT AGC^GCT
Asp718I 442 GGTACC G^GTACC
AspEI 255 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1061 CCTAGG C^CTAGG
BamHI 71 GGATCC G^GATCC
BclI 2389 TGATCA T^GATCA
BglII 877 AGATCT A^GATCT
BlnI 1061 CCTAGG C^CTAGG
BsaAI 426 TACGTG [CT]AC^GT[GA]
BsaWI 3787 ACCGGA [AT]^CCGG[AT]
BsmI 1758 GAATGC GAATGC(1/-1)
Bsp1407I 2185 TGTACA T^GTACA
BspLU11I 4322 ACATGT A^CATGT
BsrGI 2185 TGTACA T^GTACA
Eam1105I 255 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1117 AGCGCT AGC^GCT
FbaI 2389 TGATCA T^GATCA
HindIII 4938 AAGCTT A^AGCTT
KpnI 442 GGTACC GGTAC^C
NheI 3696 GCTAGC G^CTAGC
PaeR7I 1669 CTCGAG C^TCGAG
PshAI 810 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 356 GAGCTC GAGCT^C
SalI 4520 GTCGAC G^TCGAC
SapI 4683 GCTCTTC GCTCTTC(1/4)
SbfI 3332 CCTGCAGG CCTGCA^GG
SexAI 256 ACCTGGT A^CC[AT]GGT
SgrAI 169 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1285 CCCGGG CCC^GGG
SphI 3916 GCATGC GCATG^C
Sse8387I 3332 CCTGCAGG CCTGCA^GG
SspBI 2185 TGTACA T^GTACA
SspI 5020 AATATT AAT^ATT
XhoI 1669 CTCGAG C^TCGAG
XmaCI 1285 CCCGGG C^CCGGG
XmaI 1285 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 3035 GGGCCC GGGCC^C
4743 GGGCCC
Asp700I 1922 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2921 GAAGTTCTTC
AspI 1020 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2034 GACATCGTC
BcgI 1373 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
556 GCACGGTGATCG
BlpI 920 GCTCAGC GC^T[ACGT]AGC
1098 GCTCAGC
Bpu1102I 920 GCTCAGC GC^T[ACGT]AGC
1098 GCTCAGC
BsiEI 1904 CGGCCG CG[GA][CT]^CG
4880 CGGCCG
BstEII 1544 GGTGACC G^GT[ACGT]ACC
1709 GGTGACC
BstPI 1544 GGTGACC G^GT[ACGT]ACC
1709 GGTGACC
CelII 920 GCTCAGC GC^T[ACGT]AGC
1098 GCTCAGC
DraIII 1274 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3751 CACCCAGTG
EagI 1904 CGGCCG C^GGCCG
4880 CGGCCG
EclXI 1904 CGGCCG C^GGCCG
4880 CGGCCG
Eco52I 1904 CGGCCG C^GGCCG
4880 CGGCCG
EcoNI 3245 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3902 CCTGCTAGAGG
EcoO65I 1544 GGTGACC G^GT[ACGT]ACC
1709 GGTGACC
PflFI 1020 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2034 GACATCGTC
Tth111I 1020 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2034 GACATCGTC
XmnI 1922 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2921 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII