##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AccI        AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp700I     BciVI       BclI        BfrI        BsaBI       
BsiWI       Bsp1407I    BspDI       BsrGI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
DraIII      DrdI        EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KspI        MamI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AcsI         3008  AAATTT               [GA]^AATT[CT]
AhdI         5778  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         3008  AAATTT               [GA]^AATT[CT]
AspEI        5778  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1968  TGCGCA               TGC^GCA
BamHI         462  GGATCC               G^GATCC
BbsI         3187  GAAGAC               GAAGAC(2/6)
BcgI         4533  CGATTCTCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2700  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     2700  GCTTAGC              GC^T[ACGT]AGC
BpuAI        3187  GAAGAC               GAAGAC(2/6)
BspHI         852  TCATGA               T^CATGA
BspLU11I      564  ACATGT               A^CATGT
BssSI        5031  CTCGTG               CACGAG(-5/-1)
CelII        2700  GCTTAGC              GC^T[ACGT]AGC
CpoI          252  CGGTCCG              CG^G[AT]CCG
EagI          662  CGGCCG               C^GGCCG
Eam1105I     5778  GACTTTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         662  CGGCCG               C^GGCCG
Eco52I        662  CGGCCG               C^GGCCG
FspI         1968  TGCGCA               TGC^GCA
HincII        545  GTCAAC               GT[CT]^[GA]AC
HindII        545  GTCAAC               GT[CT]^[GA]AC
PaeR7I       3066  CTCGAG               C^TCGAG
RcaI          852  TCATGA               T^CATGA
RsrII         252  CGGTCCG              CG^G[AT]CCG
ScaI          690  AGTACT               AGT^ACT
XbaI          616  TCTAGA               T^CTAGA
XhoI         3066  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Acc65I       2187  GGTACC               G^GTACC
             4358  GGTACC              
Asp718I      2187  GGTACC               G^GTACC
             4358  GGTACC              
AvrII        4838  CCTAGG               C^CTAGG
             5813  CCTAGG              
BbrPI         136  CACGTG               CAC^GTG
              901  CACGTG              
BglII         678  AGATCT               A^GATCT
             1473  AGATCT              
BlnI         4838  CCTAGG               C^CTAGG
             5813  CCTAGG              
BsiEI         610  CGACCG               CG[GA][CT]^CG
              662  CGGCCG              
BsmBI        5672  CGTCTC               CGTCTC(1/5)
             4615  GAGACG              
BsmI         1930  GAATGC               GAATGC(1/-1)
             3102  GCATTC              
BsrDI        4239  CATTGC               GCAATG(2/0)
             2889  GCAATG              
DraI         3005  TTTAAA               TTT^AAA
             3213  TTTAAA              
KpnI         2187  GGTACC               GGTAC^C
             4358  GGTACC              
NaeI         3836  GCCGGC               GCC^GGC
             5586  GCCGGC              
NgoMIV       3836  GCCGGC               G^CCGGC
             5586  GCCGGC              
NspI          564  ACATGT               [GA]CATG^[CT]
             1260  ACATGC              
PmaCI         136  CACGTG               CAC^GTG
              901  CACGTG              
PmlI          136  CACGTG               CAC^GTG
              901  CACGTG              
PshAI        3553  GACAGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3862  GACATTAGTC          


#########################################
# 3 OR MORE CUT ENZYMES (  144 enzymes) #
#########################################

AccII       AccIII      AciI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
Aor51HI     ApaI        AspHI       AspI        AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaAI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspEI       BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco47III    Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmaI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI