################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccI AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I BciVI BclI BfrI BsaBI BsiWI Bsp1407I BspDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraIII DrdI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcsI 3008 AAATTT [GA]^AATT[CT] AhdI 5778 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 3008 AAATTT [GA]^AATT[CT] AspEI 5778 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1968 TGCGCA TGC^GCA BamHI 462 GGATCC G^GATCC BbsI 3187 GAAGAC GAAGAC(2/6) BcgI 4533 CGATTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 2700 GCTTAGC GC^T[ACGT]AGC Bpu1102I 2700 GCTTAGC GC^T[ACGT]AGC BpuAI 3187 GAAGAC GAAGAC(2/6) BspHI 852 TCATGA T^CATGA BspLU11I 564 ACATGT A^CATGT BssSI 5031 CTCGTG CACGAG(-5/-1) CelII 2700 GCTTAGC GC^T[ACGT]AGC CpoI 252 CGGTCCG CG^G[AT]CCG EagI 662 CGGCCG C^GGCCG Eam1105I 5778 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 662 CGGCCG C^GGCCG Eco52I 662 CGGCCG C^GGCCG FspI 1968 TGCGCA TGC^GCA HincII 545 GTCAAC GT[CT]^[GA]AC HindII 545 GTCAAC GT[CT]^[GA]AC PaeR7I 3066 CTCGAG C^TCGAG RcaI 852 TCATGA T^CATGA RsrII 252 CGGTCCG CG^G[AT]CCG ScaI 690 AGTACT AGT^ACT XbaI 616 TCTAGA T^CTAGA XhoI 3066 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Acc65I 2187 GGTACC G^GTACC 4358 GGTACC Asp718I 2187 GGTACC G^GTACC 4358 GGTACC AvrII 4838 CCTAGG C^CTAGG 5813 CCTAGG BbrPI 136 CACGTG CAC^GTG 901 CACGTG BglII 678 AGATCT A^GATCT 1473 AGATCT BlnI 4838 CCTAGG C^CTAGG 5813 CCTAGG BsiEI 610 CGACCG CG[GA][CT]^CG 662 CGGCCG BsmBI 5672 CGTCTC CGTCTC(1/5) 4615 GAGACG BsmI 1930 GAATGC GAATGC(1/-1) 3102 GCATTC BsrDI 4239 CATTGC GCAATG(2/0) 2889 GCAATG DraI 3005 TTTAAA TTT^AAA 3213 TTTAAA KpnI 2187 GGTACC GGTAC^C 4358 GGTACC NaeI 3836 GCCGGC GCC^GGC 5586 GCCGGC NgoMIV 3836 GCCGGC G^CCGGC 5586 GCCGGC NspI 564 ACATGT [GA]CATG^[CT] 1260 ACATGC PmaCI 136 CACGTG CAC^GTG 901 CACGTG PmlI 136 CACGTG CAC^GTG 901 CACGTG PshAI 3553 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3862 GACATTAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 144 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI