##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
ApaLI       AseI        AsnI        Asp700I     AviII       
BbsI        BcgI        BciVI       BclI        BfrI        
BpuAI       BsiWI       BsmI        BspDI       BsrDI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        DraIII      EcoRV       FbaI        FspI        
HincII      HindII      HindIII     HpaI        MluI        
NheI        NruI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       2605  GGTACC               G^GTACC
AccI         4544  GTCTAC               GT^[AC][GT]AC
AhdI          125  GACACGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI          419  GGCGCGCC             GG^CGCGCC
Asp718I      2605  GGTACC               G^GTACC
AspEI         125  GACACGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3351  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        5196  GGATCC               G^GATCC
BbeI          313  GGCGCC               GGCGC^C
BbrPI        1935  CACGTG               CAC^GTG
BsaBI        3483  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I     5692  TGTACA               T^GTACA
BspHI        1706  TCATGA               T^CATGA
BspLU11I     1838  ACATGT               A^CATGT
BsrGI        5692  TGTACA               T^GTACA
BssSI         643  CTCGTG               CACGAG(-5/-1)
BstEII         82  GGTGACC              G^GT[ACGT]ACC
BstPI          82  GGTGACC              G^GT[ACGT]ACC
DrdI         1719  GACAAGTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      125  GACACGCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I        82  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1893  ATGCAT               ATGCA^T
FseI         3166  GGCCGGCC             GGCCGG^CC
KasI          313  GGCGCC               G^GCGCC
KpnI         2605  GGTACC               GGTAC^C
MamI         3483  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         4270  CAATTG               C^AATTG
MunI         4270  CAATTG               C^AATTG
NarI          313  GGCGCC               GG^CGCC
NdeI         1861  CATATG               CA^TATG
NotI            1  GCGGCCGC             GC^GGCCGC
NsiI         1893  ATGCAT               ATGCA^T
PaeR7I       3527  CTCGAG               C^TCGAG
PflFI        3351  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1935  CACGTG               CAC^GTG
PmlI         1935  CACGTG               CAC^GTG
RcaI         1706  TCATGA               T^CATGA
SfiI         5019  GGCCGAGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          313  GGCGCC               GGC^GCC
SphI         1487  GCATGC               GCATG^C
SspBI        5692  TGTACA               T^GTACA
Tth111I      3351  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3527  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccIII       3347  TCCGGA               T^CCGGA
             5471  TCCGGA              
AocI         2245  CCTCAGG              CC^T[ACGT]AGG
             2600  CCTGAGG             
BglII        4409  AGATCT               A^GATCT
             5066  AGATCT              
BsaAI         181  TACGTG               [CT]AC^GT[GA]
             1935  CACGTG              
BseAI        3347  TCCGGA               T^CCGGA
             5471  TCCGGA              
BsiEI           2  CGGCCG               CG[GA][CT]^CG
             4664  CGGCCG              
BspEI        3347  TCCGGA               T^CCGGA
             5471  TCCGGA              
BssHII        362  GCGCGC               G^CGCGC
              420  GCGCGC              
Bsu36I       2245  CCTCAGG              CC^T[ACGT]AGG
             2600  CCTGAGG             
EagI            2  CGGCCG               C^GGCCG
             4664  CGGCCG              
EclXI           2  CGGCCG               C^GGCCG
             4664  CGGCCG              
Eco52I          2  CGGCCG               C^GGCCG
             4664  CGGCCG              
Eco81I       2245  CCTCAGG              CC^T[ACGT]AGG
             2600  CCTGAGG             
EcoNI        2402  CCTCTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4176  CCTGCGAGAGG         
EcoRI        1844  GAATTC               G^AATTC
             2815  GAATTC              
MroI         3347  TCCGGA               T^CCGGA
             5471  TCCGGA              
MseI         1477  TTAA                 T^TAA
             5713  TTAA                
NaeI          917  GCCGGC               GCC^GGC
             3167  GCCGGC              
NcoI         3062  CCATGG               C^CATGG
             3810  CCATGG              
NgoMIV        917  GCCGGC               G^CCGGC
             3167  GCCGGC              
Tru9I        1477  TTAA                 T^TAA
             5713  TTAA                
XcmI         3204  CCACGGCCCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3870  CCACTCAGACAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  133 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       Aor51HI     
ApaI        ApoI        AspHI       AvaI        AvaII       
AvrII       BalI        BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco47III    EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI