##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvrII
BciVI BclI BfrI BlnI BsaBI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eco47III EcoRI EcoRV EcoT22I FbaI
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NotI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
ScaI SexAI SfuI SnaBI SpeI
SspBI SspI SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 3224 ACGTGT A^C[GA][CT]GT
AgeI 3080 ACCGGT A^CCGGT
AviII 3513 TGCGCA TGC^GCA
BamHI 3815 GGATCC G^GATCC
BbrPI 3223 CACGTG CAC^GTG
BbsI 4013 GAAGAC GAAGAC(2/6)
BpuAI 4013 GAAGAC GAAGAC(2/6)
BsaAI 3223 CACGTG [CT]AC^GT[GA]
BsaWI 3080 ACCGGT [AT]^CCGG[AT]
BspHI 4327 TCATGA T^CATGA
BssSI 5256 CTCGTG CACGAG(-5/-1)
BstEII 5313 GGTAACC G^GT[ACGT]ACC
BstPI 5313 GGTAACC G^GT[ACGT]ACC
EcoO65I 5313 GGTAACC G^GT[ACGT]ACC
FspI 3513 TGCGCA TGC^GCA
HincII 2950 GTCGAC GT[CT]^[GA]AC
HindII 2950 GTCGAC GT[CT]^[GA]AC
MslI 712 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 3928 GCTAGC G^CTAGC
NruI 4095 TCGCGA TCG^CGA
PinAI 3080 ACCGGT A^CCGGT
PmaCI 3223 CACGTG CAC^GTG
PmlI 3223 CACGTG CAC^GTG
RcaI 4327 TCATGA T^CATGA
SalI 2950 GTCGAC G^TCGAC
SfiI 2925 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2391 CGCCGGCG C[GA]^CCGG[CT]G
SphI 3866 GCATGC GCATG^C
XbaI 159 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 2950 GTCGAC GT^[AC][GT]AC
4676 GTAGAC
AhdI 697 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1423 GACCCCTCGTC
Alw44I 3220 GTGCAC G^TGCAC
3766 GTGCAC
ApaLI 3220 GTGCAC G^TGCAC
3766 GTGCAC
AspEI 697 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1423 GACCCCTCGTC
BglII 407 AGATCT A^GATCT
584 AGATCT
BsmI 1098 GCATTC GAATGC(1/-1)
1343 GCATTC
BssHII 467 GCGCGC G^CGCGC
1254 GCGCGC
BstAPI 231 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1656 GCACCCCCTGC
DrdI 2941 GACCGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3075 GACGCACCGGTC
Eam1105I 697 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1423 GACCCCTCGTC
FseI 964 GGCCGGCC GGCCGG^CC
2730 GGCCGGCC
HindIII 154 AAGCTT A^AGCTT
3659 AAGCTT
MseI 3311 TTAA T^TAA
3902 TTAA
SbfI 228 CCTGCAGG CCTGCA^GG
3138 CCTGCAGG
Sse8387I 228 CCTGCAGG CCTGCA^GG
3138 CCTGCAGG
Tru9I 3311 TTAA T^TAA
3902 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI