################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AscI Asp718I AspI AviII AvrII BbeI BcgI BglI BlnI BsaBI BsaWI BseAI BsiWI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 1347 CGCG CG^CG AcyI 875 GACGCC G[GA]^CG[CT]C AflII 1734 CTTAAG C^TTAAG AhdI 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2020 CCTCAGG CC^T[ACGT]AGG Aor51HI 2854 AGCGCT AGC^GCT ApaI 424 GGGCCC GGGCC^C AspEI 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 243 TGGCCA TGG^CCA BamHI 3630 GGATCC G^GATCC BanI 2216 GGCACC G^G[CT][GA]CC BbvCI 1984 GCTGAGG CCTCAGC(-5/-2) BciVI 690 GGATAC GTATCC(6/5) BfrI 1734 CTTAAG C^TTAAG BlpI 4353 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4353 GCTCAGC GC^T[ACGT]AGC BsaHI 875 GACGCC G[GA]^CG[CT]C BsmBI 324 CGTCTC CGTCTC(1/5) BspMI 2342 GCAGGT ACCTGC(4/8) BsrDI 3836 GCAATG GCAATG(2/0) BstEII 1800 GGTCACC G^GT[ACGT]ACC BstPI 1800 GGTCACC G^GT[ACGT]ACC BstUI 1347 CGCG CG^CG BstXI 3678 CCATGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2020 CCTCAGG CC^T[ACGT]AGG CelII 4353 GCTCAGC GC^T[ACGT]AGC CfoI 2855 GCGC GCG^C DrdI 2198 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1391 CGGCCG C^GGCCG Eam1105I 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1391 CGGCCG C^GGCCG Eco47III 2854 AGCGCT AGC^GCT Eco52I 1391 CGGCCG C^GGCCG Eco81I 2020 CCTCAGG CC^T[ACGT]AGG EcoNI 4099 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1800 GGTCACC G^GT[ACGT]ACC EcoRI 3862 GAATTC G^AATTC EcoRV 1618 GATATC GAT^ATC HaeII 2854 AGCGCT [GA]GCGC^[CT] HgaI 875 GACGC GACGC(5/10) HhaI 2855 GCGC GCG^C Hin1I 875 GACGCC G[GA]^CG[CT]C HinP1I 2855 GCGC G^CGC MluNI 243 TGGCCA TGG^CCA MscI 243 TGGCCA TGG^CCA MvnI 1347 CGCG CG^CG NaeI 256 GCCGGC GCC^GGC NgoMIV 256 GCCGGC G^CCGGC PaeR7I 2722 CTCGAG C^TCGAG SacI 2590 GAGCTC GAGCT^C SalI 585 GTCGAC G^TCGAC ScaI 161 AGTACT AGT^ACT SmaI 2813 CCCGGG CCC^GGG XcmI 1476 CCACTATGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2722 CTCGAG C^TCGAG XmaCI 2813 CCCGGG C^CCGGG XmaI 2813 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 585 GTCGAC GT^[AC][GT]AC 3672 GTCTAC AcsI 2736 GAATTT [GA]^AATT[CT] 3862 GAATTC Alw44I 67 GTGCAC G^TGCAC 3767 GTGCAC AlwNI 2918 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 3479 CAGCTACTG ApaLI 67 GTGCAC G^TGCAC 3767 GTGCAC ApoI 2736 GAATTT [GA]^AATT[CT] 3862 GAATTC AseI 4378 ATTAAT AT^TAAT 4602 ATTAAT AsnI 4378 ATTAAT AT^TAAT 4602 ATTAAT Asp700I 1076 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4090 GAATGTTTTC BbrPI 70 CACGTG CAC^GTG 4737 CACGTG BclI 2661 TGATCA T^GATCA 3372 TGATCA BglII 1609 AGATCT A^GATCT 2035 AGATCT BsaI 2725 GAGACC GGTCTC(1/5) 3608 GAGACC BsiEI 587 CGACCG CG[GA][CT]^CG 1391 CGGCCG Bsp1407I 1262 TGTACA T^GTACA 2333 TGTACA BspHI 563 TCATGA T^CATGA 1517 TCATGA BsrFI 256 GCCGGC [GA]^CCGG[CT] 3763 GCCGGT BsrGI 1262 TGTACA T^GTACA 2333 TGTACA BstAPI 2307 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3760 GCAGCCGGTGC Cfr10I 256 GCCGGC [GA]^CCGG[CT] 3763 GCCGGT DraIII 1477 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 1553 CACCAAGTG FbaI 2661 TGATCA T^GATCA 3372 TGATCA HincII 585 GTCGAC GT[CT]^[GA]AC 3106 GTCAAC HindII 585 GTCGAC GT[CT]^[GA]AC 3106 GTCAAC HindIII 3589 AAGCTT A^AGCTT 3746 AAGCTT NheI 433 GCTAGC G^CTAGC 4077 GCTAGC PflMI 3184 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3652 CCACACCATGG PmaCI 70 CACGTG CAC^GTG 4737 CACGTG PmlI 70 CACGTG CAC^GTG 4737 CACGTG PshAI 645 GACATTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3563 GACAACTGTC PshBI 4378 ATTAAT AT^TAAT 4602 ATTAAT PstI 1523 CTGCAG CTGCA^G 2386 CTGCAG PvuII 1982 CAGCTG CAG^CTG 2411 CAGCTG RcaI 563 TCATGA T^CATGA 1517 TCATGA SspBI 1262 TGTACA T^GTACA 2333 TGTACA SspI 2490 AATATT AAT^ATT 2993 AATATT StuI 1179 AGGCCT AGG^CCT 2642 AGGCCT Van91I 3184 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3652 CCACACCATGG XmnI 1076 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4090 GAATGTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII