##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp718I AspEI AviII
BbrPI BbvCI BclI BglII BsaBI
BsaI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfuI SnaBI SpeI StuI SwaI
Tsp509I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1093 GTCTAC GT^[AC][GT]AC
AflII 2617 CTTAAG C^TTAAG
BamHI 2583 GGATCC G^GATCC
BciVI 686 GTATCC GTATCC(6/5)
BfrI 2617 CTTAAG C^TTAAG
BlpI 3042 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3042 GCTCAGC GC^T[ACGT]AGC
BsrDI 2444 CATTGC GCAATG(2/0)
BssHII 3239 GCGCGC G^CGCGC
BstEII 2340 GGTGACC G^GT[ACGT]ACC
BstPI 2340 GGTGACC G^GT[ACGT]ACC
CelII 3042 GCTCAGC GC^T[ACGT]AGC
DrdI 1084 GACGACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 187 CGGCCG C^GGCCG
EclXI 187 CGGCCG C^GGCCG
Eco52I 187 CGGCCG C^GGCCG
EcoO65I 2340 GGTGACC G^GT[ACGT]ACC
EcoRV 263 GATATC GAT^ATC
NdeI 3386 CATATG CA^TATG
SacI 2812 GAGCTC GAGCT^C
SapI 2464 GAAGAGC GCTCTTC(1/4)
SbfI 1296 CCTGCAGG CCTGCA^GG
ScaI 67 AGTACT AGT^ACT
SfiI 886 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2449 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 1296 CCTGCAGG CCTGCA^GG
SspI 3733 AATATT AAT^ATT
TaqI 452 TCGA T^CGA
TfiI 3118 GAATC G^A[AT]TC
TthHB8I 452 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 592 GGCGCC G[GA]^CG[CT]C
1517 GGCGCC
AflIII 1358 ACATGT A^C[GA][CT]GT
3197 ACATGT
Alw44I 798 GTGCAC G^TGCAC
3227 GTGCAC
AocI 55 CCTGAGG CC^T[ACGT]AGG
2962 CCTCAGG
ApaLI 798 GTGCAC G^TGCAC
3227 GTGCAC
Asp700I 46 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3473 GAATGTTTTC
AspI 1087 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1912 GACTGTGTC
AvrII 3462 CCTAGG C^CTAGG
3673 CCTAGG
BalI 503 TGGCCA TGG^CCA
1980 TGGCCA
BbeI 592 GGCGCC GGCGC^C
1517 GGCGCC
BlnI 3462 CCTAGG C^CTAGG
3673 CCTAGG
BsaAI 586 TACGTG [CT]AC^GT[GA]
2755 TACGTG
BsaHI 592 GGCGCC G[GA]^CG[CT]C
1517 GGCGCC
BsiEI 187 CGGCCG CG[GA][CT]^CG
1665 CGACCG
BsmBI 1721 GAGACG CGTCTC(1/5)
2829 GAGACG
Bsp1407I 3382 TGTACA T^GTACA
3590 TGTACA
BspLU11I 1358 ACATGT A^CATGT
3197 ACATGT
BsrBI 1854 CCGCTC CCGCTC(-3/-3)
1111 GAGCGG
BsrGI 3382 TGTACA T^GTACA
3590 TGTACA
Bsu36I 55 CCTGAGG CC^T[ACGT]AGG
2962 CCTCAGG
DraIII 1654 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3060 CACTTGGTG
Eco81I 55 CCTGAGG CC^T[ACGT]AGG
2962 CCTCAGG
EcoNI 55 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1767 CCTGATTGAGG
HaeII 592 GGCGCC [GA]GCGC^[CT]
1517 GGCGCC
Hin1I 592 GGCGCC G[GA]^CG[CT]C
1517 GGCGCC
HincII 808 GTCAAC GT[CT]^[GA]AC
1951 GTCAAC
HindII 808 GTCAAC GT[CT]^[GA]AC
1951 GTCAAC
KasI 592 GGCGCC G^GCGCC
1517 GGCGCC
MluNI 503 TGGCCA TGG^CCA
1980 TGGCCA
MscI 503 TGGCCA TGG^CCA
1980 TGGCCA
MseI 1502 TTAA T^TAA
2618 TTAA
NaeI 1846 GCCGGC GCC^GGC
2450 GCCGGC
NarI 592 GGCGCC GG^CGCC
1517 GGCGCC
NgoMIV 1846 GCCGGC G^CCGGC
2450 GCCGGC
PflFI 1087 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1912 GACTGTGTC
PflMI 1653 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2217 CCAGGCCCTGG
SexAI 1655 ACCTGGT A^CC[AT]GGT
2105 ACCTGGT
SfoI 592 GGCGCC GGC^GCC
1517 GGCGCC
SmlI 1735 CTCAAG C^T[CT][GA]AG
2617 CTTAAG
SphI 1240 GCATGC GCATG^C
2634 GCATGC
SspBI 3382 TGTACA T^GTACA
3590 TGTACA
Tru9I 1502 TTAA T^TAA
2618 TTAA
Tsp45I 1355 GTGAC ^GT[GC]AC
2341 GTGAC
Tth111I 1087 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1912 GACTGTGTC
Van91I 1653 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2217 CCAGGCCCTGG
XmnI 46 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3473 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TseI TspRI
XcmI XhoII XmaCI XmaI