##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaLI       
AscI        Asp700I     Asp718I     AviII       AvrII       
BbeI        BciVI       BclI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BseAI       BsiWI       BsmBI       BspEI       BspLU11I    
BssHII      BstBI       BstEII      BstPI       BstXI       
CelII       CpoI        DrdI        Eco47III    EcoO65I     
EcoRV       EcoT22I     FbaI        FspI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NarI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       RsrII       SalI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspI        SwaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AclI         2222  AACGTT               AA^CGTT
AhdI         1411  GACCACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1474  CCTCAGG              CC^T[ACGT]AGG
AseI         3781  ATTAAT               AT^TAAT
AsnI         3781  ATTAAT               AT^TAAT
AspEI        1411  GACCACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          589  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2695  TGGCCA               TGG^CCA
BamHI        1086  GGATCC               G^GATCC
BbrPI        1386  CACGTG               CAC^GTG
BbvCI        3018  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1488  CGACCGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI         2209  GCCCCAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1386  CACGTG               [CT]AC^GT[GA]
BsaWI        1787  ACCGGA               [AT]^CCGG[AT]
Bsp1407I     3229  TGTACA               T^GTACA
BspDI        1246  ATCGAT               AT^CGAT
BspHI        1405  TCATGA               T^CATGA
BsrBI         632  CCGCTC               CCGCTC(-3/-3)
BsrFI        3026  GCCGGC               [GA]^CCGG[CT]
BsrGI        3229  TGTACA               T^GTACA
BssSI        3485  CTCGTG               CACGAG(-5/-1)
BstAPI       2757  GCAGTGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1474  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       3026  GCCGGC               [GA]^CCGG[CT]
ClaI         1246  ATCGAT               AT^CGAT
EagI         3024  CGGCCG               C^GGCCG
Eam1105I     1411  GACCACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3024  CGGCCG               C^GGCCG
Eco52I       3024  CGGCCG               C^GGCCG
Eco81I       1474  CCTCAGG              CC^T[ACGT]AGG
EcoRI        2348  GAATTC               G^AATTC
FseI         3025  GGCCGGCC             GGCCGG^CC
KspI          759  CCGCGG               CCGC^GG
MluNI        2695  TGGCCA               TGG^CCA
MscI         2695  TGGCCA               TGG^CCA
NaeI         3026  GCCGGC               GCC^GGC
NdeI         1239  CATATG               CA^TATG
NgoMIV       3026  GCCGGC               G^CCGGC
PflFI         589  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1386  CACGTG               CAC^GTG
PmlI         1386  CACGTG               CAC^GTG
PshBI        3781  ATTAAT               AT^TAAT
Psp1406I     2222  AACGTT               AA^CGTT
PvuI         1248  CGATCG               CGAT^CG
RcaI         1405  TCATGA               T^CATGA
SacII         759  CCGCGG               CCGC^GG
SapI         1572  GCTCTTC              GCTCTTC(1/4)
SbfI         2381  CCTGCAGG             CCTGCA^GG
SexAI        1859  ACCAGGT              A^CC[AT]GGT
SmaI          939  CCCGGG               CCC^GGG
Sse8387I     2381  CCTGCAGG             CCTGCA^GG
SspBI        3229  TGTACA               T^GTACA
Tth111I       589  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          245  TCTAGA               T^CTAGA
XmaCI         939  CCCGGG               C^CCGGG
XmaI          939  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaI          939  CCCGGG               C^[CT]CG[GA]G
             1399  CCCGAG              
BbsI          416  GAAGAC               GAAGAC(2/6)
             3405  GAAGAC              
BpuAI         416  GAAGAC               GAAGAC(2/6)
             3405  GAAGAC              
BsoBI         939  CCCGGG               C^[CT]CG[GA]G
             1399  CCCGAG              
Bst1107I      464  GTATAC               GTA^TAC
             3696  GTATAC              
BstZ17I       464  GTATAC               GTA^TAC
             3696  GTATAC              
CfoI          714  GCGC                 GCG^C
             2116  GCGC                
DraIII       1858  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2982  CACAGTGTG           
HaeII         713  AGCGCC               [GA]GCGC^[CT]
             2115  GGCGCT              
HgaI          959  GCGTC                GACGC(5/10)
             1648  GCGTC               
HhaI          714  GCGC                 GCG^C
             2116  GCGC                
HinP1I        714  GCGC                 G^CGC
             2116  GCGC                
MslI          227  CACTGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              807  CATTGTGGTG          
NspI         1132  GCATGT               [GA]CATG^[CT]
             1483  ACATGC              
PvuII        2353  CAGCTG               CAG^CTG
             3588  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspHI       AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII