################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI Asp700I Asp718I AviII AvrII BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiWI BsmBI BspEI BspLU11I BssHII BstBI BstEII BstPI BstXI CelII CpoI DrdI Eco47III EcoO65I EcoRV EcoT22I FbaI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI RsrII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AclI 2222 AACGTT AA^CGTT AhdI 1411 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1474 CCTCAGG CC^T[ACGT]AGG AseI 3781 ATTAAT AT^TAAT AsnI 3781 ATTAAT AT^TAAT AspEI 1411 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 589 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2695 TGGCCA TGG^CCA BamHI 1086 GGATCC G^GATCC BbrPI 1386 CACGTG CAC^GTG BbvCI 3018 CCTCAGC CCTCAGC(-5/-2) BcgI 1488 CGACCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2209 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1386 CACGTG [CT]AC^GT[GA] BsaWI 1787 ACCGGA [AT]^CCGG[AT] Bsp1407I 3229 TGTACA T^GTACA BspDI 1246 ATCGAT AT^CGAT BspHI 1405 TCATGA T^CATGA BsrBI 632 CCGCTC CCGCTC(-3/-3) BsrFI 3026 GCCGGC [GA]^CCGG[CT] BsrGI 3229 TGTACA T^GTACA BssSI 3485 CTCGTG CACGAG(-5/-1) BstAPI 2757 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1474 CCTCAGG CC^T[ACGT]AGG Cfr10I 3026 GCCGGC [GA]^CCGG[CT] ClaI 1246 ATCGAT AT^CGAT EagI 3024 CGGCCG C^GGCCG Eam1105I 1411 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3024 CGGCCG C^GGCCG Eco52I 3024 CGGCCG C^GGCCG Eco81I 1474 CCTCAGG CC^T[ACGT]AGG EcoRI 2348 GAATTC G^AATTC FseI 3025 GGCCGGCC GGCCGG^CC KspI 759 CCGCGG CCGC^GG MluNI 2695 TGGCCA TGG^CCA MscI 2695 TGGCCA TGG^CCA NaeI 3026 GCCGGC GCC^GGC NdeI 1239 CATATG CA^TATG NgoMIV 3026 GCCGGC G^CCGGC PflFI 589 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1386 CACGTG CAC^GTG PmlI 1386 CACGTG CAC^GTG PshBI 3781 ATTAAT AT^TAAT Psp1406I 2222 AACGTT AA^CGTT PvuI 1248 CGATCG CGAT^CG RcaI 1405 TCATGA T^CATGA SacII 759 CCGCGG CCGC^GG SapI 1572 GCTCTTC GCTCTTC(1/4) SbfI 2381 CCTGCAGG CCTGCA^GG SexAI 1859 ACCAGGT A^CC[AT]GGT SmaI 939 CCCGGG CCC^GGG Sse8387I 2381 CCTGCAGG CCTGCA^GG SspBI 3229 TGTACA T^GTACA Tth111I 589 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 245 TCTAGA T^CTAGA XmaCI 939 CCCGGG C^CCGGG XmaI 939 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaI 939 CCCGGG C^[CT]CG[GA]G 1399 CCCGAG BbsI 416 GAAGAC GAAGAC(2/6) 3405 GAAGAC BpuAI 416 GAAGAC GAAGAC(2/6) 3405 GAAGAC BsoBI 939 CCCGGG C^[CT]CG[GA]G 1399 CCCGAG Bst1107I 464 GTATAC GTA^TAC 3696 GTATAC BstZ17I 464 GTATAC GTA^TAC 3696 GTATAC CfoI 714 GCGC GCG^C 2116 GCGC DraIII 1858 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG 2982 CACAGTGTG HaeII 713 AGCGCC [GA]GCGC^[CT] 2115 GGCGCT HgaI 959 GCGTC GACGC(5/10) 1648 GCGTC HhaI 714 GCGC GCG^C 2116 GCGC HinP1I 714 GCGC G^CGC 2116 GCGC MslI 227 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 807 CATTGTGGTG NspI 1132 GCATGT [GA]CATG^[CT] 1483 ACATGC PvuII 2353 CAGCTG CAG^CTG 3588 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII