################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AatII Acc65I AclI AgeI Asp718I AspI BamHI BcgI BglII BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV FseI HpaI KpnI KspI MluI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AflII 3269 CTTAAG C^TTAAG Alw44I 2176 GTGCAC G^TGCAC Aor51HI 2300 AGCGCT AGC^GCT ApaLI 2176 GTGCAC G^TGCAC AscI 100 GGCGCGCC GG^CGCGCC AseI 3861 ATTAAT AT^TAAT AsnI 3861 ATTAAT AT^TAAT Asp700I 3564 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2064 TGCGCA TGC^GCA AvrII 3749 CCTAGG C^CTAGG BbrPI 1806 CACGTG CAC^GTG BciVI 3644 GGATAC GTATCC(6/5) BfrI 3269 CTTAAG C^TTAAG BlnI 3749 CCTAGG C^CTAGG BsaAI 1806 CACGTG [CT]AC^GT[GA] BsaBI 1203 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 4041 TCATGA T^CATGA BssHII 101 GCGCGC G^CGCGC BssSI 3234 CACGAG CACGAG(-5/-1) DraI 1891 TTTAAA TTT^AAA DraIII 3054 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2300 AGCGCT AGC^GCT EcoNI 2190 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 952 ATGCAT ATGCA^T FspI 2064 TGCGCA TGC^GCA MamI 1203 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2801 CAATTG C^AATTG MunI 2801 CAATTG C^AATTG NsiI 952 ATGCAT ATGCA^T PaeR7I 2320 CTCGAG C^TCGAG PmaCI 1806 CACGTG CAC^GTG PmlI 1806 CACGTG CAC^GTG PshBI 3861 ATTAAT AT^TAAT RcaI 4041 TCATGA T^CATGA SacI 152 GAGCTC GAGCT^C SapI 1117 GAAGAGC GCTCTTC(1/4) SmaI 138 CCCGGG CCC^GGG SphI 2067 GCATGC GCATG^C StuI 1970 AGGCCT AGG^CCT XhoI 2320 CTCGAG C^TCGAG XmaCI 138 CCCGGG C^CCGGG XmaI 138 CCCGGG C^CCGGG XmnI 3564 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 1387 GTCTAC GT^[AC][GT]AC 2463 GTAGAC AccIII 728 TCCGGA T^CCGGA 2312 TCCGGA AhdI 2466 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4099 GACTCTTGGTC AspEI 2466 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4099 GACTCTTGGTC BbvCI 563 CCTCAGC CCTCAGC(-5/-2) 919 GCTGAGG BclI 605 TGATCA T^GATCA 3451 TGATCA BsaWI 728 TCCGGA [AT]^CCGG[AT] 2312 TCCGGA BseAI 728 TCCGGA T^CCGGA 2312 TCCGGA BspEI 728 TCCGGA T^CCGGA 2312 TCCGGA BspLU11I 1508 ACATGT A^CATGT 2643 ACATGT BsrFI 1391 ACCGGC [GA]^CCGG[CT] 2258 ACCGGC Cfr10I 1391 ACCGGC [GA]^CCGG[CT] 2258 ACCGGC DrdI 114 GACGCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2200 GACCTCTTTGTC Eam1105I 2466 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4099 GACTCTTGGTC FbaI 605 TGATCA T^GATCA 3451 TGATCA HincII 4082 GTTGAC GT[CT]^[GA]AC 4178 GTCAAC HindII 4082 GTTGAC GT[CT]^[GA]AC 4178 GTCAAC MaeII 1310 ACGT A^CGT 1807 ACGT MroI 728 TCCGGA T^CCGGA 2312 TCCGGA PshAI 1710 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4113 GACCTGGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII