##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AgeI Asp718I
AspI BamHI BcgI BglII BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoRI
EcoRV FseI HpaI KpnI KspI
MluI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 3269 CTTAAG C^TTAAG
Alw44I 2176 GTGCAC G^TGCAC
Aor51HI 2300 AGCGCT AGC^GCT
ApaLI 2176 GTGCAC G^TGCAC
AscI 100 GGCGCGCC GG^CGCGCC
AseI 3861 ATTAAT AT^TAAT
AsnI 3861 ATTAAT AT^TAAT
Asp700I 3564 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2064 TGCGCA TGC^GCA
AvrII 3749 CCTAGG C^CTAGG
BbrPI 1806 CACGTG CAC^GTG
BciVI 3644 GGATAC GTATCC(6/5)
BfrI 3269 CTTAAG C^TTAAG
BlnI 3749 CCTAGG C^CTAGG
BsaAI 1806 CACGTG [CT]AC^GT[GA]
BsaBI 1203 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 4041 TCATGA T^CATGA
BssHII 101 GCGCGC G^CGCGC
BssSI 3234 CACGAG CACGAG(-5/-1)
DraI 1891 TTTAAA TTT^AAA
DraIII 3054 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2300 AGCGCT AGC^GCT
EcoNI 2190 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 952 ATGCAT ATGCA^T
FspI 2064 TGCGCA TGC^GCA
MamI 1203 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2801 CAATTG C^AATTG
MunI 2801 CAATTG C^AATTG
NsiI 952 ATGCAT ATGCA^T
PaeR7I 2320 CTCGAG C^TCGAG
PmaCI 1806 CACGTG CAC^GTG
PmlI 1806 CACGTG CAC^GTG
PshBI 3861 ATTAAT AT^TAAT
RcaI 4041 TCATGA T^CATGA
SacI 152 GAGCTC GAGCT^C
SapI 1117 GAAGAGC GCTCTTC(1/4)
SmaI 138 CCCGGG CCC^GGG
SphI 2067 GCATGC GCATG^C
StuI 1970 AGGCCT AGG^CCT
XhoI 2320 CTCGAG C^TCGAG
XmaCI 138 CCCGGG C^CCGGG
XmaI 138 CCCGGG C^CCGGG
XmnI 3564 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1387 GTCTAC GT^[AC][GT]AC
2463 GTAGAC
AccIII 728 TCCGGA T^CCGGA
2312 TCCGGA
AhdI 2466 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4099 GACTCTTGGTC
AspEI 2466 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4099 GACTCTTGGTC
BbvCI 563 CCTCAGC CCTCAGC(-5/-2)
919 GCTGAGG
BclI 605 TGATCA T^GATCA
3451 TGATCA
BsaWI 728 TCCGGA [AT]^CCGG[AT]
2312 TCCGGA
BseAI 728 TCCGGA T^CCGGA
2312 TCCGGA
BspEI 728 TCCGGA T^CCGGA
2312 TCCGGA
BspLU11I 1508 ACATGT A^CATGT
2643 ACATGT
BsrFI 1391 ACCGGC [GA]^CCGG[CT]
2258 ACCGGC
Cfr10I 1391 ACCGGC [GA]^CCGG[CT]
2258 ACCGGC
DrdI 114 GACGCGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACCTCTTTGTC
Eam1105I 2466 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4099 GACTCTTGGTC
FbaI 605 TGATCA T^GATCA
3451 TGATCA
HincII 4082 GTTGAC GT[CT]^[GA]AC
4178 GTCAAC
HindII 4082 GTTGAC GT[CT]^[GA]AC
4178 GTCAAC
MaeII 1310 ACGT A^CGT
1807 ACGT
MroI 728 TCCGGA T^CCGGA
2312 TCCGGA
PshAI 1710 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4113 GACCTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII