##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        Asp718I     
AspI        BamHI       BcgI        BglII       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BsrGI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        EagI        EclXI       Eco52I      EcoRI       
EcoRV       FseI        HpaI        KpnI        KspI        
MluI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmeI        Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AflII        3269  CTTAAG               C^TTAAG
Alw44I       2176  GTGCAC               G^TGCAC
Aor51HI      2300  AGCGCT               AGC^GCT
ApaLI        2176  GTGCAC               G^TGCAC
AscI          100  GGCGCGCC             GG^CGCGCC
AseI         3861  ATTAAT               AT^TAAT
AsnI         3861  ATTAAT               AT^TAAT
Asp700I      3564  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        2064  TGCGCA               TGC^GCA
AvrII        3749  CCTAGG               C^CTAGG
BbrPI        1806  CACGTG               CAC^GTG
BciVI        3644  GGATAC               GTATCC(6/5)
BfrI         3269  CTTAAG               C^TTAAG
BlnI         3749  CCTAGG               C^CTAGG
BsaAI        1806  CACGTG               [CT]AC^GT[GA]
BsaBI        1203  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI        4041  TCATGA               T^CATGA
BssHII        101  GCGCGC               G^CGCGC
BssSI        3234  CACGAG               CACGAG(-5/-1)
DraI         1891  TTTAAA               TTT^AAA
DraIII       3054  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2300  AGCGCT               AGC^GCT
EcoNI        2190  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       952  ATGCAT               ATGCA^T
FspI         2064  TGCGCA               TGC^GCA
MamI         1203  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2801  CAATTG               C^AATTG
MunI         2801  CAATTG               C^AATTG
NsiI          952  ATGCAT               ATGCA^T
PaeR7I       2320  CTCGAG               C^TCGAG
PmaCI        1806  CACGTG               CAC^GTG
PmlI         1806  CACGTG               CAC^GTG
PshBI        3861  ATTAAT               AT^TAAT
RcaI         4041  TCATGA               T^CATGA
SacI          152  GAGCTC               GAGCT^C
SapI         1117  GAAGAGC              GCTCTTC(1/4)
SmaI          138  CCCGGG               CCC^GGG
SphI         2067  GCATGC               GCATG^C
StuI         1970  AGGCCT               AGG^CCT
XhoI         2320  CTCGAG               C^TCGAG
XmaCI         138  CCCGGG               C^CCGGG
XmaI          138  CCCGGG               C^CCGGG
XmnI         3564  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI         1387  GTCTAC               GT^[AC][GT]AC
             2463  GTAGAC              
AccIII        728  TCCGGA               T^CCGGA
             2312  TCCGGA              
AhdI         2466  GACTGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4099  GACTCTTGGTC         
AspEI        2466  GACTGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4099  GACTCTTGGTC         
BbvCI         563  CCTCAGC              CCTCAGC(-5/-2)
              919  GCTGAGG             
BclI          605  TGATCA               T^GATCA
             3451  TGATCA              
BsaWI         728  TCCGGA               [AT]^CCGG[AT]
             2312  TCCGGA              
BseAI         728  TCCGGA               T^CCGGA
             2312  TCCGGA              
BspEI         728  TCCGGA               T^CCGGA
             2312  TCCGGA              
BspLU11I     1508  ACATGT               A^CATGT
             2643  ACATGT              
BsrFI        1391  ACCGGC               [GA]^CCGG[CT]
             2258  ACCGGC              
Cfr10I       1391  ACCGGC               [GA]^CCGG[CT]
             2258  ACCGGC              
DrdI          114  GACGCGGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2200  GACCTCTTTGTC        
Eam1105I     2466  GACTGGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4099  GACTCTTGGTC         
FbaI          605  TGATCA               T^GATCA
             3451  TGATCA              
HincII       4082  GTTGAC               GT[CT]^[GA]AC
             4178  GTCAAC              
HindII       4082  GTTGAC               GT[CT]^[GA]AC
             4178  GTCAAC              
MaeII        1310  ACGT                 A^CGT
             1807  ACGT                
MroI          728  TCCGGA               T^CCGGA
             2312  TCCGGA              
PshAI        1710  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4113  GACCTGGGTC          


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       AocI        
ApaI        ApoI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbsI        
BbvI        BcnI        BfaI        BglI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrI        
BssKI       BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII