##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BamHI       BbrPI       BciVI       BclI        
BfrI        BlnI        BsaBI       BsmI        Bsp1407I    
BspDI       BspLU11I    BspMI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
Eam1105I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccI         2217  GTCGAC               GT^[AC][GT]AC
AccIII       1990  TCCGGA               T^CCGGA
AcsI         1286  AAATTT               [GA]^AATT[CT]
AflIII        622  ACGTGT               A^C[GA][CT]GT
ApoI         1286  AAATTT               [GA]^AATT[CT]
AviII        3238  TGCGCA               TGC^GCA
BcgI          566  GCAAGTGTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI         882  TACGTG               [CT]AC^GT[GA]
BseAI        1990  TCCGGA               T^CCGGA
BsiEI          23  CGGCCG               CG[GA][CT]^CG
BsiWI         880  CGTACG               C^GTACG
BspEI        1990  TCCGGA               T^CCGGA
BspHI        2984  TCATGA               T^CATGA
DraIII       1851  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI           23  CGGCCG               C^GGCCG
EclXI          23  CGGCCG               C^GGCCG
Eco52I         23  CGGCCG               C^GGCCG
FspI         3238  TGCGCA               TGC^GCA
HincII       2217  GTCGAC               GT[CT]^[GA]AC
HindII       2217  GTCGAC               GT[CT]^[GA]AC
MroI         1990  TCCGGA               T^CCGGA
NdeI         2056  CATATG               CA^TATG
PflMI        3669  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2468  GACCATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2984  TCATGA               T^CATGA
SalI         2217  GTCGAC               G^TCGAC
SexAI        1852  ACCTGGT              A^CC[AT]GGT
SfiI         3568  GGCCCCCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Van91I       3669  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Aor51HI      1033  AGCGCT               AGC^GCT
             2398  AGCGCT              
BalI          232  TGGCCA               TGG^CCA
             3324  TGGCCA              
BbvCI         326  GCTGAGG              CCTCAGC(-5/-2)
             3826  GCTGAGG             
BfaI         1185  CTAG                 C^TAG
             3705  CTAG                
BglII         796  AGATCT               A^GATCT
             2614  AGATCT              
BlpI          759  GCTCAGC              GC^T[ACGT]AGC
             1707  GCTGAGC             
Bpu1102I      759  GCTCAGC              GC^T[ACGT]AGC
             1707  GCTGAGC             
BsgI         3487  CTGCAC               GTGCAG(16/14)
              885  GTGCAG              
CelII         759  GCTCAGC              GC^T[ACGT]AGC
             1707  GCTGAGC             
Eco47III     1033  AGCGCT               AGC^GCT
             2398  AGCGCT              
EcoT14I      1672  CCATGG               C^C[AT][AT]GG
             2581  CCATGG              
MaeI         1185  CTAG                 C^TAG
             3705  CTAG                
MluNI         232  TGGCCA               TGG^CCA
             3324  TGGCCA              
MscI          232  TGGCCA               TGG^CCA
             3324  TGGCCA              
MseI          397  TTAA                 T^TAA
             4196  TTAA                
NaeI           21  GCCGGC               GCC^GGC
             3406  GCCGGC              
NcoI         1672  CCATGG               C^CATGG
             2581  CCATGG              
NgoMIV         21  GCCGGC               G^CCGGC
             3406  GCCGGC              
StuI         1517  AGGCCT               AGG^CCT
             4131  AGGCCT              
StyI         1672  CCATGG               C^C[AT][AT]GG
             2581  CCATGG              
TfiI         2306  GAATC                G^A[AT]TC
             2879  GATTC               
Tru9I         397  TTAA                 T^TAA
             4196  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BsrBI       BsrDI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        SmlI        SphI        
TaqI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       XmaCI       XmaI