##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccII AcyI AflII AgeI
AhdI Alw44I ApaLI AscI AspEI
AspI AviII BbeI BcgI BclI
BfrI BglI BglII BsaHI BsiEI
BsiWI BsmBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Cfr10I
CpoI EagI Eam1105I EclXI Eco52I
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI KspI MluI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PpuMI PshAI
PstI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 74 enzymes) #
###################################
Acc65I 2708 GGTACC G^GTACC
AccI 3257 GTCTAC GT^[AC][GT]AC
AccIII 1543 TCCGGA T^CCGGA
AclI 2045 AACGTT AA^CGTT
AocI 3377 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4087 AGCGCT AGC^GCT
ApaI 3264 GGGCCC GGGCC^C
Asp718I 2708 GGTACC G^GTACC
AvaI 104 CTCGAG C^[CT]CG[GA]G
AvrII 3604 CCTAGG C^CTAGG
BalI 2535 TGGCCA TGG^CCA
BbrPI 497 CACGTG CAC^GTG
BbvCI 3359 GCTGAGG CCTCAGC(-5/-2)
BciVI 617 GTATCC GTATCC(6/5)
BcnI 1689 CCCGG CC^[GC]GG
BlnI 3604 CCTAGG C^CTAGG
BpmI 3620 CTGGAG CTGGAG(16/14)
BsaAI 497 CACGTG [CT]AC^GT[GA]
BsaBI 1026 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1543 TCCGGA [AT]^CCGG[AT]
BseAI 1543 TCCGGA T^CCGGA
BsgI 3324 GTGCAG GTGCAG(16/14)
BsmI 548 GCATTC GAATGC(1/-1)
BsoBI 104 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3703 TGTACA T^GTACA
BspDI 2807 ATCGAT AT^CGAT
BspEI 1543 TCCGGA T^CCGGA
BspHI 270 TCATGA T^CATGA
BspLU11I 375 ACATGT A^CATGT
BsrDI 996 GCAATG GCAATG(2/0)
BsrGI 3703 TGTACA T^GTACA
BstAPI 3049 GCAGTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2815 GGTGACC G^GT[ACGT]ACC
BstPI 2815 GGTGACC G^GT[ACGT]ACC
Bsu36I 3377 CCTGAGG CC^T[ACGT]AGG
CfoI 4088 GCGC GCG^C
ClaI 2807 ATCGAT AT^CGAT
DraI 642 TTTAAA TTT^AAA
DraIII 2120 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 238 GACACTCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2535 TGGCCA [CT]^GGCC[GA]
Eco47III 4087 AGCGCT AGC^GCT
Eco81I 3377 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2815 GGTGACC G^GT[ACGT]ACC
EcoRI 1944 GAATTC G^AATTC
EcoRV 883 GATATC GAT^ATC
FauI 1296 CCCGC CCCGC(4/6)
HaeII 4087 AGCGCT [GA]GCGC^[CT]
HgaI 1540 GCGTC GACGC(5/10)
HhaI 4088 GCGC GCG^C
HinP1I 4088 GCGC G^CGC
HindIII 3309 AAGCTT A^AGCTT
KpnI 2708 GGTACC GGTAC^C
MamI 1026 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2535 TGGCCA TGG^CCA
MroI 1543 TCCGGA T^CCGGA
MscI 2535 TGGCCA TGG^CCA
NciI 1689 CCCGG CC^[GC]GG
NcoI 3483 CCATGG C^CATGG
NheI 81 GCTAGC G^CTAGC
PaeR7I 104 CTCGAG C^TCGAG
PmaCI 497 CACGTG CAC^GTG
PmlI 497 CACGTG CAC^GTG
Psp1406I 2045 AACGTT AA^CGTT
RcaI 270 TCATGA T^CATGA
SacI 2981 GAGCTC GAGCT^C
SapI 2450 GCTCTTC GCTCTTC(1/4)
ScaI 2011 AGTACT AGT^ACT
SspBI 3703 TGTACA T^GTACA
SspI 1018 AATATT AAT^ATT
StuI 3923 AGGCCT AGG^CCT
Tsp45I 2816 GTGAC ^GT[GC]AC
XcmI 912 CCAAGATAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 104 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AseI 1727 ATTAAT AT^TAAT
2633 ATTAAT
AsnI 1727 ATTAAT AT^TAAT
2633 ATTAAT
AspHI 500 GTGCTC G[AT]GC[AT]^C
2981 GAGCTC
BamHI 366 GGATCC G^GATCC
3187 GGATCC
BanII 2981 GAGCTC G[GA]GC[CT]^C
3264 GGGCCC
BlpI 502 GCTCAGC GC^T[ACGT]AGC
1171 GCTGAGC
Bpu1102I 502 GCTCAGC GC^T[ACGT]AGC
1171 GCTGAGC
BsiHKAI 500 GTGCTC G[AT]GC[AT]^C
2981 GAGCTC
BsrBI 107 GAGCGG CCGCTC(-3/-3)
1712 GAGCGG
CelII 502 GCTCAGC GC^T[ACGT]AGC
1171 GCTGAGC
DraII 3263 AGGGCCC [GA]G^G[ACGT]CC[CT]
3264 GGGCCCT
DsaI 3246 CCGTGG C^C[GA][CT]GG
3483 CCATGG
EcoNI 1465 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1783 CCTGATGCAGG
EcoO109I 3263 AGGGCCC [GA]G^G[ACGT]CC[CT]
3264 GGGCCCT
HapII 1544 CCGG C^CGG
1690 CCGG
HpaII 1544 CCGG C^CGG
1690 CCGG
MfeI 51 CAATTG C^AATTG
3627 CAATTG
MspA1I 386 CAGCTG C[AC]G^C[GT]G
3357 CAGCTG
MspI 1544 CCGG C^CGG
1690 CCGG
MunI 51 CAATTG C^AATTG
3627 CAATTG
NspI 375 ACATGT [GA]CATG^[CT]
3889 ACATGC
PshBI 1727 ATTAAT AT^TAAT
2633 ATTAAT
PvuII 386 CAGCTG CAG^CTG
3357 CAGCTG
SmlI 104 CTCGAG C^T[CT][GA]AG
2987 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbsI BbvI BfaI BmyI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII XmnI