##################################
# NO CUT ENZYMES (   62 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
Asp718I     AvrII       BbrPI       BbsI        BfrI        
BlnI        BpuAI       BsaAI       BsaBI       BseAI       
BsiWI       BspDI       BspEI       BsrDI       BstAPI      
BstBI       BstXI       ClaI        CpoI        DraIII      
EcoRV       EcoT22I     FseI        HincII      HindII      
HpaI        KpnI        MamI        MluI        MroI        
NcoI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI         2016  GTATAC               GT^[AC][GT]AC
AgeI         2193  ACCGGT               A^CCGGT
AhdI         2158  GACCACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3497  GTGCAC               G^TGCAC
Aor51HI       670  AGCGCT               AGC^GCT
ApaLI        3497  GTGCAC               G^TGCAC
AscI          898  GGCGCGCC             GG^CGCGCC
AspEI        2158  GACCACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3519  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4013  TGCGCA               TGC^GCA
BcgI          287  CGAGTTGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3022  TGATCA               T^GATCA
BpmI         1599  CTGGAG               CTGGAG(16/14)
BsmBI         267  GAGACG               CGTCTC(1/5)
Bsp1407I     3916  TGTACA               T^GTACA
BspHI        1058  TCATGA               T^CATGA
BspMI        1674  GCAGGT               ACCTGC(4/8)
BsrGI        3916  TGTACA               T^GTACA
Bst1107I     2016  GTATAC               GTA^TAC
BstEII       3880  GGTGACC              G^GT[ACGT]ACC
BstPI        3880  GGTGACC              G^GT[ACGT]ACC
BstZ17I      2016  GTATAC               GTA^TAC
Eam1105I     2158  GACCACCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      670  AGCGCT               AGC^GCT
EcoO65I      3880  GGTGACC              G^GT[ACGT]ACC
EcoRI         975  GAATTC               G^AATTC
FbaI         3022  TGATCA               T^GATCA
FspI         4013  TGCGCA               TGC^GCA
PflFI        3519  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        2193  ACCGGT               A^CCGGT
PstI         2399  CTGCAG               CTGCA^G
RcaI         1058  TCATGA               T^CATGA
SacI         1519  GAGCTC               GAGCT^C
SapI         2760  GCTCTTC              GCTCTTC(1/4)
ScaI          160  AGTACT               AGT^ACT
SmlI         3278  CTCAAG               C^T[CT][GA]AG
SphI         1653  GCATGC               GCATG^C
SspBI        3916  TGTACA               T^GTACA
Tth111I      3519  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1515  CCAGGAGCTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AocI         1585  CCTGAGG              CC^T[ACGT]AGG
             2368  CCTCAGG             
Asp700I       536  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              971  GAATGAATTC          
BalI          675  TGGCCA               TGG^CCA
             2500  TGGCCA              
BamHI         583  GGATCC               G^GATCC
             3166  GGATCC              
BciVI        3244  GTATCC               GTATCC(6/5)
             3673  GTATCC              
BglII         601  AGATCT               A^GATCT
             1750  AGATCT              
BlpI         1832  GCTCAGC              GC^T[ACGT]AGC
             2662  GCTCAGC             
Bpu1102I     1832  GCTCAGC              GC^T[ACGT]AGC
             2662  GCTCAGC             
BsaWI        2193  ACCGGT               [AT]^CCGG[AT]
             2733  TCCGGT              
BsmI         2709  GCATTC               GAATGC(1/-1)
             3404  GCATTC              
BstF5I        293  GGATG                GGATG(2/0)
              802  GGATG               
Bsu36I       1585  CCTGAGG              CC^T[ACGT]AGG
             2368  CCTCAGG             
CelII        1832  GCTCAGC              GC^T[ACGT]AGC
             2662  GCTCAGC             
DrdI         1116  GACAATAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3555  GACATAAGGGTC        
Eco81I       1585  CCTGAGG              CC^T[ACGT]AGG
             2368  CCTCAGG             
EcoNI        2343  CCTCAGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4080  CCTCTCTGAGG         
FokI          293  GGATG                GGATG(9/13)
              802  GGATG               
HindIII      3887  AAGCTT               A^AGCTT
             3968  AAGCTT              
MfeI         1850  CAATTG               C^AATTG
             4233  CAATTG              
MluNI         675  TGGCCA               TGG^CCA
             2500  TGGCCA              
MscI          675  TGGCCA               TGG^CCA
             2500  TGGCCA              
MunI         1850  CAATTG               C^AATTG
             4233  CAATTG              
NheI           57  GCTAGC               G^CTAGC
             3310  GCTAGC              
PflMI         318  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3315  CCACAATTTGG         
StuI         1576  AGGCCT               AGG^CCT
             2365  AGGCCT              
TaqI          514  TCGA                 T^CGA
             1766  TCGA                
TthHB8I       514  TCGA                 T^CGA
             1766  TCGA                
Van91I        318  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3315  CCACAATTTGG         
XmnI          536  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              971  GAATGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  127 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApaI        
ApoI        AseI        AsnI        AspHI       AvaI        
AvaII       BanI        BanII       BbeI        BbvCI       
BbvI        BcnI        BfaI        BglI        BmyI        
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspLU11I    BsrBI       
BsrFI       BsrI        BssHII      BssKI       BssSI       
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PpuMI       
PshBI       PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
XmaCI       XmaI