##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AspI BbvCI
BcgI BclI BfrI BglII BsaBI
BseAI BsiWI BspEI BspHI BspLU11I
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI DraI EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI KspI MamI
MfeI MroI MunI NdeI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1932 GGTACC G^GTACC
AccI 2796 GTCTAC GT^[AC][GT]AC
AcsI 1248 GAATTC [GA]^AATT[CT]
Alw44I 2826 GTGCAC G^TGCAC
ApaLI 2826 GTGCAC G^TGCAC
ApoI 1248 GAATTC [GA]^AATT[CT]
Asp700I 2876 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1932 GGTACC G^GTACC
BamHI 2890 GGATCC G^GATCC
BbeI 2472 GGCGCC GGCGC^C
BbrPI 133 CACGTG CAC^GTG
BciVI 1184 GGATAC GTATCC(6/5)
BsiEI 3712 CGACCG CG[GA][CT]^CG
BsmI 2969 GAATGC GAATGC(1/-1)
BspDI 1428 ATCGAT AT^CGAT
BsrDI 1570 GCAATG GCAATG(2/0)
BsrFI 3670 GCCGGC [GA]^CCGG[CT]
BstAPI 2542 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1743 TTCGAA TT^CGAA
Cfr10I 3670 GCCGGC [GA]^CCGG[CT]
ClaI 1428 ATCGAT AT^CGAT
DraIII 751 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3054 GACTGGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1248 GAATTC G^AATTC
FseI 3669 GGCCGGCC GGCCGG^CC
KasI 2472 GGCGCC G^GCGCC
KpnI 1932 GGTACC GGTAC^C
NaeI 3670 GCCGGC GCC^GGC
NarI 2472 GGCGCC GG^CGCC
NcoI 3265 CCATGG C^CATGG
NgoMIV 3670 GCCGGC G^CCGGC
NheI 3853 GCTAGC G^CTAGC
NspV 1743 TTCGAA TT^CGAA
PaeR7I 691 CTCGAG C^TCGAG
PmaCI 133 CACGTG CAC^GTG
PmlI 133 CACGTG CAC^GTG
ScaI 1372 AGTACT AGT^ACT
SexAI 1188 ACCTGGT A^CC[AT]GGT
SfoI 2472 GGCGCC GGC^GCC
SfuI 1743 TTCGAA TT^CGAA
SphI 458 GCATGC GCATG^C
Tsp509I 1249 AATT ^AATT
XhoI 691 CTCGAG C^TCGAG
XmnI 2876 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 2472 GGCGCC G[GA]^CG[CT]C
4065 GGCGTC
AhdI 2160 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3723 GACGCGGTGTC
AocI 1403 CCTGAGG CC^T[ACGT]AGG
3761 CCTCAGG
Aor51HI 3204 AGCGCT AGC^GCT
3395 AGCGCT
AspEI 2160 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3723 GACGCGGTGTC
AviII 2198 TGCGCA TGC^GCA
3043 TGCGCA
BsaAI 77 TACGTG [CT]AC^GT[GA]
133 CACGTG
BsaHI 2472 GGCGCC G[GA]^CG[CT]C
4065 GGCGTC
BsaWI 964 ACCGGA [AT]^CCGG[AT]
1922 ACCGGA
BsgI 1873 CTGCAC GTGCAG(16/14)
63 GTGCAG
BstXI 591 CCACGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1105 CCAATGGGCTGG
Bsu36I 1403 CCTGAGG CC^T[ACGT]AGG
3761 CCTCAGG
Eam1105I 2160 GACACCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3723 GACGCGGTGTC
Eco47III 3204 AGCGCT AGC^GCT
3395 AGCGCT
Eco81I 1403 CCTGAGG CC^T[ACGT]AGG
3761 CCTCAGG
FspI 2198 TGCGCA TGC^GCA
3043 TGCGCA
Hin1I 2472 GGCGCC G[GA]^CG[CT]C
4065 GGCGTC
MluI 34 ACGCGT A^CGCGT
57 ACGCGT
MseI 2379 TTAA T^TAA
4163 TTAA
NspI 458 GCATGC [GA]CATG^[CT]
640 GCATGT
SacI 818 GAGCTC GAGCT^C
2961 GAGCTC
SfiI 1457 GGCCGACTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2246 GGCCGATGTGGCC
Tru9I 2379 TTAA T^TAA
4163 TTAA
XcmI 1993 CCAGAGCCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2755 CCAGCCCTTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI