##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspI BamHI BbeI BfrI
BsaBI BsaWI BseAI BsiWI BspEI
BspHI BspLU11I Bst1107I BstBI BstZ17I
CpoI DrdI DsaI Eco47III EcoRI
EcoRV EcoT22I FseI KasI KspI
MamI MluI MroI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 3913 GACGTC GACGT^C
Acc65I 3370 GGTACC G^GTACC
AflIII 1351 ACGTGT A^C[GA][CT]GT
AhdI 2848 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 689 CCTCAGG CC^T[ACGT]AGG
Asp700I 1935 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3370 GGTACC G^GTACC
AspEI 2848 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1550 TGCGCA TGC^GCA
BbrPI 1867 CACGTG CAC^GTG
BclI 1638 TGATCA T^GATCA
BglII 1074 AGATCT A^GATCT
BlpI 3290 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3290 GCTCAGC GC^T[ACGT]AGC
BspDI 3147 ATCGAT AT^CGAT
BsrBI 2436 CCGCTC CCGCTC(-3/-3)
BsrFI 23 GCCGGC [GA]^CCGG[CT]
BssSI 3333 CACGAG CACGAG(-5/-1)
BstAPI 3973 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4386 GGTTACC G^GT[ACGT]ACC
BstPI 4386 GGTTACC G^GT[ACGT]ACC
Bsu36I 689 CCTCAGG CC^T[ACGT]AGG
CelII 3290 GCTCAGC GC^T[ACGT]AGC
Cfr10I 23 GCCGGC [GA]^CCGG[CT]
ClaI 3147 ATCGAT AT^CGAT
DraIII 2863 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2848 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 689 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4386 GGTTACC G^GT[ACGT]ACC
FbaI 1638 TGATCA T^GATCA
FspI 1550 TGCGCA TGC^GCA
HaeII 2362 AGCGCC [GA]GCGC^[CT]
HindIII 465 AAGCTT A^AGCTT
HpaI 4258 GTTAAC GTT^AAC
KpnI 3370 GGTACC GGTAC^C
NaeI 23 GCCGGC GCC^GGC
NgoMIV 23 GCCGGC G^CCGGC
PaeR7I 1469 CTCGAG C^TCGAG
PmaCI 1867 CACGTG CAC^GTG
PmlI 1867 CACGTG CAC^GTG
PpuMI 3106 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3913 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3145 CGATCG CGAT^CG
SmaI 2919 CCCGGG CCC^GGG
StuI 4356 AGGCCT AGG^CCT
XhoI 1469 CTCGAG C^TCGAG
XmaCI 2919 CCCGGG C^CCGGG
XmaI 2919 CCCGGG C^CCGGG
XmnI 1935 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 3442 GGGCCC GGGCC^C
3573 GGGCCC
AvrII 4101 CCTAGG C^CTAGG
4359 CCTAGG
BcgI 164 GCACCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2577 GCAATCCATTCG
BciVI 3850 GGATAC GTATCC(6/5)
4427 GGATAC
BglI 2758 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3078 GCCAGATGGGC
BlnI 4101 CCTAGG C^CTAGG
4359 CCTAGG
BsaAI 1350 TACGTG [CT]AC^GT[GA]
1867 CACGTG
BsaI 2010 GAGACC GGTCTC(1/5)
1296 GGTCTC
BsgI 747 GTGCAG GTGCAG(16/14)
2701 GTGCAG
BsmBI 32 GAGACG CGTCTC(1/5)
3911 GAGACG
BsmI 2352 GCATTC GAATGC(1/-1)
2957 GCATTC
BsrDI 572 CATTGC GCAATG(2/0)
2639 GCAATG
BstXI 1442 CCACCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1997 CCAGGTATTTGG
EagI 14 CGGCCG C^GGCCG
2311 CGGCCG
EclXI 14 CGGCCG C^GGCCG
2311 CGGCCG
Eco52I 14 CGGCCG C^GGCCG
2311 CGGCCG
HincII 2964 GTCAAC GT[CT]^[GA]AC
4258 GTTAAC
HindII 2964 GTCAAC GT[CT]^[GA]AC
4258 GTTAAC
PflMI 1722 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCACATTGTGG
SacI 1239 GAGCTC GAGCT^C
1508 GAGCTC
SpeI 249 ACTAGT A^CTAGT
2995 ACTAGT
SspI 4176 AATATT AAT^ATT
4307 AATATT
Van91I 1722 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2862 CCACATTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII