##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflIII      
AgeI        Aor51HI     AscI        Asp700I     Asp718I     
AviII       AvrII       BamHI       BbrPI       BciVI       
BlnI        BsaAI       BsaBI       BsiWI       BsmI        
BspDI       BspLU11I    BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       FseI        FspI        HpaI        
KpnI        KspI        MamI        MluI        NaeI        
NdeI        NgoMIV      NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    RsrII       SacII       SalI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccIII       1273  TCCGGA               T^CCGGA
AcyI         3092  GGCGCC               G[GA]^CG[CT]C
AflII        3005  CTTAAG               C^TTAAG
AseI          369  ATTAAT               AT^TAAT
AsnI          369  ATTAAT               AT^TAAT
BbeI         3092  GGCGCC               GGCGC^C
BfrI         3005  CTTAAG               C^TTAAG
BsaHI        3092  GGCGCC               G[GA]^CG[CT]C
BseAI        1273  TCCGGA               T^CCGGA
BsiEI        1392  CGATCG               CG[GA][CT]^CG
BspEI        1273  TCCGGA               T^CCGGA
BsrFI        2764  ACCGGC               [GA]^CCGG[CT]
Cfr10I       2764  ACCGGC               [GA]^CCGG[CT]
DraIII       4837  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      3639  ATGCAT               ATGCA^T
Hin1I        3092  GGCGCC               G[GA]^CG[CT]C
KasI         3092  GGCGCC               G^GCGCC
MfeI         5800  CAATTG               C^AATTG
MroI         1273  TCCGGA               T^CCGGA
MunI         5800  CAATTG               C^AATTG
NarI         3092  GGCGCC               GG^CGCC
NheI         6095  GCTAGC               G^CTAGC
NsiI         3639  ATGCAT               ATGCA^T
PaeR7I       1006  CTCGAG               C^TCGAG
PshBI         369  ATTAAT               AT^TAAT
PvuI         1392  CGATCG               CGAT^CG
SbfI         3180  CCTGCAGG             CCTGCA^GG
SfoI         3092  GGCGCC               GGC^GCC
SmaI         3154  CCCGGG               CCC^GGG
Sse8387I     3180  CCTGCAGG             CCTGCA^GG
SspI         5268  AATATT               AAT^ATT
XbaI         4226  TCTAGA               T^CTAGA
XhoI         1006  CTCGAG               C^TCGAG
XmaCI        3154  CCCGGG               C^CCGGG
XmaI         3154  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII        1704  CGCG                 CG^CG
             2702  CGCG                
AhdI         2439  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3480  GACAAAGGGTC         
Alw44I        700  GTGCAC               G^TGCAC
             1878  GTGCAC              
ApaLI         700  GTGCAC               G^TGCAC
             1878  GTGCAC              
AspEI        2439  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3480  GACAAAGGGTC         
AspI          207  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4917  GACATGGTC           
AvaI         1006  CTCGAG               C^[CT]CG[GA]G
             3154  CCCGGG              
BalI         5673  TGGCCA               TGG^CCA
             6140  TGGCCA              
BglI         1914  GCCAGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5082  GCCCACCCGGC         
BsaWI         845  ACCGGA               [AT]^CCGG[AT]
             1273  TCCGGA              
BsoBI        1006  CTCGAG               C^[CT]CG[GA]G
             3154  CCCGGG              
Bsp1407I      163  TGTACA               T^GTACA
             3546  TGTACA              
BspHI         221  TCATGA               T^CATGA
             6277  TCATGA              
BsrGI         163  TGTACA               T^GTACA
             3546  TGTACA              
BstUI        1704  CGCG                 CG^CG
             2702  CGCG                
DrdI          995  GACCTTAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3120  GACAGCACCGTC        
Eam1105I     2439  GACAAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3480  GACAAAGGGTC         
EcoNI        3083  CCTCTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             5066  CCTGCGCCAGG         
MluNI        5673  TGGCCA               TGG^CCA
             6140  TGGCCA              
MscI         5673  TGGCCA               TGG^CCA
             6140  TGGCCA              
MvnI         1704  CGCG                 CG^CG
             2702  CGCG                
PflFI         207  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4917  GACATGGTC           
PflMI         252  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3373  CCACCCACTGG         
RcaI          221  TCATGA               T^CATGA
             6277  TCATGA              
SapI         1191  GAAGAGC              GCTCTTC(1/4)
             1752  GAAGAGC             
SspBI         163  TGTACA               T^GTACA
             3546  TGTACA              
Tth111I       207  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4917  GACATGGTC           
Van91I        252  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3373  CCACCCACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspHI       
AvaII       BanI        BanII       BbsI        BbvCI       
BbvI        BcgI        BclI        BcnI        BfaI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRI       EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SphI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII