################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccI AclI AflIII AgeI Aor51HI AscI Asp700I Asp718I AviII AvrII BamHI BbrPI BciVI BlnI BsaAI BsaBI BsiWI BsmI BspDI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KpnI KspI MamI MluI NaeI NdeI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccIII 1273 TCCGGA T^CCGGA AcyI 3092 GGCGCC G[GA]^CG[CT]C AflII 3005 CTTAAG C^TTAAG AseI 369 ATTAAT AT^TAAT AsnI 369 ATTAAT AT^TAAT BbeI 3092 GGCGCC GGCGC^C BfrI 3005 CTTAAG C^TTAAG BsaHI 3092 GGCGCC G[GA]^CG[CT]C BseAI 1273 TCCGGA T^CCGGA BsiEI 1392 CGATCG CG[GA][CT]^CG BspEI 1273 TCCGGA T^CCGGA BsrFI 2764 ACCGGC [GA]^CCGG[CT] Cfr10I 2764 ACCGGC [GA]^CCGG[CT] DraIII 4837 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 3639 ATGCAT ATGCA^T Hin1I 3092 GGCGCC G[GA]^CG[CT]C KasI 3092 GGCGCC G^GCGCC MfeI 5800 CAATTG C^AATTG MroI 1273 TCCGGA T^CCGGA MunI 5800 CAATTG C^AATTG NarI 3092 GGCGCC GG^CGCC NheI 6095 GCTAGC G^CTAGC NsiI 3639 ATGCAT ATGCA^T PaeR7I 1006 CTCGAG C^TCGAG PshBI 369 ATTAAT AT^TAAT PvuI 1392 CGATCG CGAT^CG SbfI 3180 CCTGCAGG CCTGCA^GG SfoI 3092 GGCGCC GGC^GCC SmaI 3154 CCCGGG CCC^GGG Sse8387I 3180 CCTGCAGG CCTGCA^GG SspI 5268 AATATT AAT^ATT XbaI 4226 TCTAGA T^CTAGA XhoI 1006 CTCGAG C^TCGAG XmaCI 3154 CCCGGG C^CCGGG XmaI 3154 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 1704 CGCG CG^CG 2702 CGCG AhdI 2439 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3480 GACAAAGGGTC Alw44I 700 GTGCAC G^TGCAC 1878 GTGCAC ApaLI 700 GTGCAC G^TGCAC 1878 GTGCAC AspEI 2439 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3480 GACAAAGGGTC AspI 207 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 4917 GACATGGTC AvaI 1006 CTCGAG C^[CT]CG[GA]G 3154 CCCGGG BalI 5673 TGGCCA TGG^CCA 6140 TGGCCA BglI 1914 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5082 GCCCACCCGGC BsaWI 845 ACCGGA [AT]^CCGG[AT] 1273 TCCGGA BsoBI 1006 CTCGAG C^[CT]CG[GA]G 3154 CCCGGG Bsp1407I 163 TGTACA T^GTACA 3546 TGTACA BspHI 221 TCATGA T^CATGA 6277 TCATGA BsrGI 163 TGTACA T^GTACA 3546 TGTACA BstUI 1704 CGCG CG^CG 2702 CGCG DrdI 995 GACCTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3120 GACAGCACCGTC Eam1105I 2439 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3480 GACAAAGGGTC EcoNI 3083 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5066 CCTGCGCCAGG MluNI 5673 TGGCCA TGG^CCA 6140 TGGCCA MscI 5673 TGGCCA TGG^CCA 6140 TGGCCA MvnI 1704 CGCG CG^CG 2702 CGCG PflFI 207 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 4917 GACATGGTC PflMI 252 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3373 CCACCCACTGG RcaI 221 TCATGA T^CATGA 6277 TCATGA SapI 1191 GAAGAGC GCTCTTC(1/4) 1752 GAAGAGC SspBI 163 TGTACA T^GTACA 3546 TGTACA Tth111I 207 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 4917 GACATGGTC Van91I 252 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3373 CCACCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaII BanI BanII BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII