##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccIII AflIII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AviII AvrII BanII BbeI BbrPI
BciVI BcnI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BssHII Bst1107I
BstBI BstZ17I CelII CfoI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI FspI
HaeII HhaI HinP1I KasI KpnI
MamI MluI MroI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PvuI RsrII SacI
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 415 GACGTC GACGT^C
AccII 1193 CGCG CG^CG
AclI 629 AACGTT AA^CGTT
Alw44I 571 GTGCAC G^TGCAC
AocI 2148 CCTCAGG CC^T[ACGT]AGG
ApaLI 571 GTGCAC G^TGCAC
AseI 552 ATTAAT AT^TAAT
AsnI 552 ATTAAT AT^TAAT
AspI 3497 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1273 CCTCAGC CCTCAGC(-5/-2)
BglI 143 GCCACAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsrDI 645 CATTGC GCAATG(2/0)
BsrFI 1583 ACCGGC [GA]^CCGG[CT]
BssSI 470 CACGAG CACGAG(-5/-1)
BstAPI 2632 GCACTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2521 GGTTACC G^GT[ACGT]ACC
BstPI 2521 GGTTACC G^GT[ACGT]ACC
BstUI 1193 CGCG CG^CG
BstXI 845 CCAGTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2148 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1583 ACCGGC [GA]^CCGG[CT]
DrdI 232 GACTCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2148 CCTCAGG CC^T[ACGT]AGG
EcoNI 3896 CCTTAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2521 GGTTACC G^GT[ACGT]ACC
EcoRV 3102 GATATC GAT^ATC
KspI 1192 CCGCGG CCGC^GG
MfeI 3571 CAATTG C^AATTG
MunI 3571 CAATTG C^AATTG
MvnI 1193 CGCG CG^CG
PflFI 3497 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 109 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1398 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 552 ATTAAT AT^TAAT
Psp1406I 629 AACGTT AA^CGTT
SacII 1192 CCGCGG CCGC^GG
SapI 3755 GCTCTTC GCTCTTC(1/4)
SphI 2654 GCATGC GCATG^C
StuI 1420 AGGCCT AGG^CCT
SwaI 183 ATTTAAAT ATTT^AAAT
Tth111I 3497 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1730 CCAGGCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1505 GTCTAC GT^[AC][GT]AC
3291 GTAGAC
AcyI 415 GACGTC G[GA]^CG[CT]C
1838 GGCGTC
AflII 2331 CTTAAG C^TTAAG
3821 CTTAAG
Asp700I 1534 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1705 GAAGCTATTC
AspHI 571 GTGCAC G[AT]GC[AT]^C
898 GAGCAC
AvaI 1220 CTCGAG C^[CT]CG[GA]G
1765 CTCGAG
BalI 1226 TGGCCA TGG^CCA
4142 TGGCCA
BclI 2403 TGATCA T^GATCA
3919 TGATCA
BfrI 2331 CTTAAG C^TTAAG
3821 CTTAAG
BsaHI 415 GACGTC G[GA]^CG[CT]C
1838 GGCGTC
BsaI 166 GAGACC GGTCTC(1/5)
2736 GGTCTC
BsaWI 2666 ACCGGA [AT]^CCGG[AT]
3128 ACCGGA
BseRI 1435 CTCCTC GAGGAG(10/8)
473 GAGGAG
BsiHKAI 571 GTGCAC G[AT]GC[AT]^C
898 GAGCAC
BsmBI 3502 CGTCTC CGTCTC(1/5)
413 GAGACG
BsoBI 1220 CTCGAG C^[CT]CG[GA]G
1765 CTCGAG
BsrBI 1353 GAGCGG CCGCTC(-3/-3)
2992 GAGCGG
EcoRI 2326 GAATTC G^AATTC
4107 GAATTC
FauI 1191 CCCGC CCCGC(4/6)
3639 CCCGC
FbaI 2403 TGATCA T^GATCA
3919 TGATCA
HgaI 444 GACGC GACGC(5/10)
1839 GCGTC
Hin1I 415 GACGTC G[GA]^CG[CT]C
1838 GGCGTC
MluNI 1226 TGGCCA TGG^CCA
4142 TGGCCA
MscI 1226 TGGCCA TGG^CCA
4142 TGGCCA
PaeR7I 1220 CTCGAG C^TCGAG
1765 CTCGAG
PstI 888 CTGCAG CTGCA^G
924 CTGCAG
PvuII 1276 CAGCTG CAG^CTG
2828 CAGCTG
ScaI 2883 AGTACT AGT^ACT
2980 AGTACT
SexAI 106 ACCAGGT A^CC[AT]GGT
2551 ACCTGGT
SspI 2645 AATATT AAT^ATT
2704 AATATT
XhoI 1220 CTCGAG C^TCGAG
1765 CTCGAG
XmnI 1534 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1705 GAAGCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BamHI BanI
BbsI BbvI BcgI BfaI BglII
BmyI BpmI BpuAI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspHI BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII