################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AclI AcsI AocI ApoI AscI AseI AsnI Asp700I BamHI BciVI BclI BsiWI BsmBI Bsp1407I BsrGI BstBI Bsu36I DraI Eco81I EcoRI EcoRV EcoT22I FbaI HindIII HpaI MluI NdeI NotI NruI NsiI NspV PacI PflMI PmeI PshBI Psp1406I PvuI SapI ScaI SexAI SfuI SnaBI SspBI SspI SwaI Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 5421 GGTACC G^GTACC AccIII 1895 TCCGGA T^CCGGA AflII 422 CTTAAG C^TTAAG AhdI 5253 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 5325 GGATC GGATC(4/5) Asp718I 5421 GGTACC G^GTACC AspEI 5253 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 3740 TGCGCA TGC^GCA AvrII 5434 CCTAGG C^CTAGG BbsI 2526 GAAGAC GAAGAC(2/6) BbvCI 5120 GCTGAGG CCTCAGC(-5/-2) BfrI 422 CTTAAG C^TTAAG BglII 2578 AGATCT A^GATCT BlnI 5434 CCTAGG C^CTAGG BpuAI 2526 GAAGAC GAAGAC(2/6) BsaBI 1211 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1895 TCCGGA T^CCGGA BspDI 1338 ATCGAT AT^CGAT BspEI 1895 TCCGGA T^CCGGA BspHI 482 TCATGA T^CATGA BspLU11I 1293 ACATGT A^CATGT BssSI 1560 CACGAG CACGAG(-5/-1) Bst1107I 524 GTATAC GTA^TAC BstEII 4914 GGTGACC G^GT[ACGT]ACC BstPI 4914 GGTGACC G^GT[ACGT]ACC BstYI 2578 AGATCT [GA]^GATC[CT] BstZ17I 524 GTATAC GTA^TAC ClaI 1338 ATCGAT AT^CGAT DrdI 691 GACCCCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 5253 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 4914 GGTGACC G^GT[ACGT]ACC FseI 3428 GGCCGGCC GGCCGG^CC FspI 3740 TGCGCA TGC^GCA KpnI 5421 GGTACC GGTAC^C MamI 1211 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3218 CAATTG C^AATTG MflI 2578 AGATCT [GA]^GATC[CT] MroI 1895 TCCGGA T^CCGGA MunI 3218 CAATTG C^AATTG NheI 1052 GCTAGC G^CTAGC PaeR7I 2447 CTCGAG C^TCGAG RcaI 482 TCATGA T^CATGA SalI 1344 GTCGAC G^TCGAC SpeI 4850 ACTAGT A^CTAGT TfiI 5375 GATTC G^A[AT]TC Tsp509I 3219 AATT ^AATT XhoI 2447 CTCGAG C^TCGAG XhoII 2578 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AatII 1692 GACGTC GACGT^C 3465 GACGTC Alw44I 1289 GTGCAC G^TGCAC 1382 GTGCAC ApaLI 1289 GTGCAC G^TGCAC 1382 GTGCAC BssHII 219 GCGCGC G^CGCGC 260 GCGCGC CpoI 112 CGGACCG CG^G[AT]CCG 540 CGGACCG HincII 1344 GTCGAC GT[CT]^[GA]AC 4405 GTCAAC HindII 1344 GTCGAC GT[CT]^[GA]AC 4405 GTCAAC NcoI 1118 CCATGG C^CATGG 1256 CCATGG PshAI 3345 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3564 GACAAGTGTC RsrII 112 CGGACCG CG^G[AT]CCG 540 CGGACCG SacI 2450 GAGCTC GAGCT^C 4947 GAGCTC SbfI 15 CCTGCAGG CCTGCA^GG 3314 CCTGCAGG SgrAI 214 CACCGGCG C[GA]^CCGG[CT]G 747 CACCGGTG Sse8387I 15 CCTGCAGG CCTGCA^GG 3314 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 148 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AflIII AgeI AluI AlwNI Aor51HI ApaI AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbrPI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PinAI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI SphI StuI StyI TaqI Tru9I TseI Tsp45I TspRI Tth111I TthHB8I XcmI XmaCI XmaI