##################################
# NO CUT ENZYMES (   48 enzymes) #
##################################

AclI        AcsI        AocI        ApoI        AscI        
AseI        AsnI        Asp700I     BamHI       BciVI       
BclI        BsiWI       BsmBI       Bsp1407I    BsrGI       
BstBI       Bsu36I      DraI        Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        HindIII     HpaI        
MluI        NdeI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PmeI        PshBI       
Psp1406I    PvuI        SapI        ScaI        SexAI       
SfuI        SnaBI       SspBI       SspI        SwaI        
Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I       5421  GGTACC               G^GTACC
AccIII       1895  TCCGGA               T^CCGGA
AflII         422  CTTAAG               C^TTAAG
AhdI         5253  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         5325  GGATC                GGATC(4/5)
Asp718I      5421  GGTACC               G^GTACC
AspEI        5253  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3740  TGCGCA               TGC^GCA
AvrII        5434  CCTAGG               C^CTAGG
BbsI         2526  GAAGAC               GAAGAC(2/6)
BbvCI        5120  GCTGAGG              CCTCAGC(-5/-2)
BfrI          422  CTTAAG               C^TTAAG
BglII        2578  AGATCT               A^GATCT
BlnI         5434  CCTAGG               C^CTAGG
BpuAI        2526  GAAGAC               GAAGAC(2/6)
BsaBI        1211  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1895  TCCGGA               T^CCGGA
BspDI        1338  ATCGAT               AT^CGAT
BspEI        1895  TCCGGA               T^CCGGA
BspHI         482  TCATGA               T^CATGA
BspLU11I     1293  ACATGT               A^CATGT
BssSI        1560  CACGAG               CACGAG(-5/-1)
Bst1107I      524  GTATAC               GTA^TAC
BstEII       4914  GGTGACC              G^GT[ACGT]ACC
BstPI        4914  GGTGACC              G^GT[ACGT]ACC
BstYI        2578  AGATCT               [GA]^GATC[CT]
BstZ17I       524  GTATAC               GTA^TAC
ClaI         1338  ATCGAT               AT^CGAT
DrdI          691  GACCCCGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     5253  GACTGGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      4914  GGTGACC              G^GT[ACGT]ACC
FseI         3428  GGCCGGCC             GGCCGG^CC
FspI         3740  TGCGCA               TGC^GCA
KpnI         5421  GGTACC               GGTAC^C
MamI         1211  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3218  CAATTG               C^AATTG
MflI         2578  AGATCT               [GA]^GATC[CT]
MroI         1895  TCCGGA               T^CCGGA
MunI         3218  CAATTG               C^AATTG
NheI         1052  GCTAGC               G^CTAGC
PaeR7I       2447  CTCGAG               C^TCGAG
RcaI          482  TCATGA               T^CATGA
SalI         1344  GTCGAC               G^TCGAC
SpeI         4850  ACTAGT               A^CTAGT
TfiI         5375  GATTC                G^A[AT]TC
Tsp509I      3219  AATT                 ^AATT
XhoI         2447  CTCGAG               C^TCGAG
XhoII        2578  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AatII        1692  GACGTC               GACGT^C
             3465  GACGTC              
Alw44I       1289  GTGCAC               G^TGCAC
             1382  GTGCAC              
ApaLI        1289  GTGCAC               G^TGCAC
             1382  GTGCAC              
BssHII        219  GCGCGC               G^CGCGC
              260  GCGCGC              
CpoI          112  CGGACCG              CG^G[AT]CCG
              540  CGGACCG             
HincII       1344  GTCGAC               GT[CT]^[GA]AC
             4405  GTCAAC              
HindII       1344  GTCGAC               GT[CT]^[GA]AC
             4405  GTCAAC              
NcoI         1118  CCATGG               C^CATGG
             1256  CCATGG              
PshAI        3345  GACAACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3564  GACAAGTGTC          
RsrII         112  CGGACCG              CG^G[AT]CCG
              540  CGGACCG             
SacI         2450  GAGCTC               GAGCT^C
             4947  GAGCTC              
SbfI           15  CCTGCAGG             CCTGCA^GG
             3314  CCTGCAGG            
SgrAI         214  CACCGGCG             C[GA]^CCGG[CT]G
              747  CACCGGTG            
Sse8387I       15  CCTGCAGG             CCTGCA^GG
             3314  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AgeI        AluI        AlwNI       Aor51HI     
ApaI        AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbeI        BbrPI       
BbvI        BcgI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspMI       BsrBI       BsrDI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EagI        EarI        EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PinAI       PleI        PmaCI       
PmlI        PpuMI       PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SfoI        SmaI        SmlI        
SphI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
XcmI        XmaCI       XmaI