##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AclI        AflII       AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        AspEI       
AspI        AvrII       BbeI        BcgI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiEI       Bsp1407I    BspDI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstEII      BstPI       BstZ17I     
CelII       ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
HindIII     HpaI        KasI        KspI        MamI        
MfeI        MluI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SbfI        
ScaI        SexAI       SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Acc65I        384  GGTACC               G^GTACC
AccI         4343  GTCGAC               GT^[AC][GT]AC
AccII        3315  CGCG                 CG^CG
AccIII       1483  TCCGGA               T^CCGGA
AcyI         1288  GACGCC               G[GA]^CG[CT]C
AflIII       4061  ACGTGT               A^C[GA][CT]GT
Alw44I       2037  GTGCAC               G^TGCAC
ApaLI        2037  GTGCAC               G^TGCAC
Asp718I       384  GGTACC               G^GTACC
AviII        2095  TGCGCA               TGC^GCA
BbrPI        4060  CACGTG               CAC^GTG
BbsI         1829  GAAGAC               GAAGAC(2/6)
BclI         2005  TGATCA               T^GATCA
BglII        1717  AGATCT               A^GATCT
BpuAI        1829  GAAGAC               GAAGAC(2/6)
BsaAI        4060  CACGTG               [CT]AC^GT[GA]
BsaHI        1288  GACGCC               G[GA]^CG[CT]C
BseAI        1483  TCCGGA               T^CCGGA
BsiWI        1967  CGTACG               C^GTACG
BsmBI        3303  GAGACG               CGTCTC(1/5)
BspEI        1483  TCCGGA               T^CCGGA
BspHI        1153  TCATGA               T^CATGA
BsrFI        2848  GCCGGC               [GA]^CCGG[CT]
BstBI         718  TTCGAA               TT^CGAA
BstUI        3315  CGCG                 CG^CG
Cfr10I       2848  GCCGGC               [GA]^CCGG[CT]
DraIII       1131  CACCATGTG            CAC[ACGT][ACGT][ACGT]^GTG
FbaI         2005  TGATCA               T^GATCA
FspI         2095  TGCGCA               TGC^GCA
HgaI         1288  GACGC                GACGC(5/10)
Hin1I        1288  GACGCC               G[GA]^CG[CT]C
KpnI          384  GGTACC               GGTAC^C
MaeII        4061  ACGT                 A^CGT
MroI         1483  TCCGGA               T^CCGGA
MvnI         3315  CGCG                 CG^CG
NaeI         2848  GCCGGC               GCC^GGC
NcoI           20  CCATGG               C^CATGG
NgoMIV       2848  GCCGGC               G^CCGGC
NspV          718  TTCGAA               TT^CGAA
PmaCI        4060  CACGTG               CAC^GTG
PmlI         4060  CACGTG               CAC^GTG
PshAI          48  GACAATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1153  TCATGA               T^CATGA
SacI         1239  GAGCTC               GAGCT^C
SalI         4343  GTCGAC               G^TCGAC
SfuI          718  TTCGAA               TT^CGAA
SphI          253  GCATGC               GCATG^C
StuI         1426  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AocI          347  CCTCAGG              CC^T[ACGT]AGG
             2688  CCTGAGG             
Asp700I        63  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4151  GAATGACTTC          
BalI           17  TGGCCA               TGG^CCA
             3964  TGGCCA              
BanI          384  GGTACC               G^G[CT][GA]CC
              505  GGCACC              
BsgI         1128  CTGCAC               GTGCAG(16/14)
             4057  CTGCAC              
BsmI         3283  GAATGC               GAATGC(1/-1)
             1595  GCATTC              
BstAPI       2087  GCAGAGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3051  GCAGGCGATGC         
Bsu36I        347  CCTCAGG              CC^T[ACGT]AGG
             2688  CCTGAGG             
Eco81I        347  CCTCAGG              CC^T[ACGT]AGG
             2688  CCTGAGG             
HincII       4052  GTCAAC               GT[CT]^[GA]AC
             4343  GTCGAC              
HindII       4052  GTCAAC               GT[CT]^[GA]AC
             4343  GTCGAC              
MluNI          17  TGGCCA               TGG^CCA
             3964  TGGCCA              
MscI           17  TGGCCA               TGG^CCA
             3964  TGGCCA              
SapI         1185  GAAGAGC              GCTCTTC(1/4)
             3186  GAAGAGC             
SfiI         1767  GGCCCCTTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3002  GGCCCCCTGGGCC       
XmnI           63  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4151  GAATGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApaI        ApoI        AspHI       AvaI        
AvaII       BamHI       BanII       BbvCI       BbvI        
BciVI       BcnI        BfaI        BglI        BmyI        
BpmI        BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmaCI       
XmaI