##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaHI BsiEI BsiWI BsmBI
BsoBI BspDI BspHI BsrBI BsrFI
BssHII BssSI BstEII BstPI BstUI
BstXI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FbaI FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SphI Sse8387I
StuI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2842 GTATAC GT^[AC][GT]AC
AccIII 1889 TCCGGA T^CCGGA
AciI 1741 GCGG CCGC(-3/-1)
AocI 3189 CCTTAGG CC^T[ACGT]AGG
ApaI 1792 GGGCCC GGGCC^C
AspHI 2048 GTGCTC G[AT]GC[AT]^C
AspI 1117 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 2142 GGACC G^G[AT]CC
BanII 1792 GGGCCC G[GA]GC[CT]^C
BbsI 3416 GTCTTC GAAGAC(2/6)
BlpI 2112 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2112 GCTGAGC GC^T[ACGT]AGC
BpuAI 3416 GTCTTC GAAGAC(2/6)
BsaBI 1213 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 796 GAGACC GGTCTC(1/5)
BseAI 1889 TCCGGA T^CCGGA
BsiHKAI 2048 GTGCTC G[AT]GC[AT]^C
BsmI 702 GAATGC GAATGC(1/-1)
BspEI 1889 TCCGGA T^CCGGA
Bst1107I 2842 GTATAC GTA^TAC
BstBI 4120 TTCGAA TT^CGAA
BstYI 908 AGATCC [GA]^GATC[CT]
BstZ17I 2842 GTATAC GTA^TAC
Bsu36I 3189 CCTTAGG CC^T[ACGT]AGG
CelII 2112 GCTGAGC GC^T[ACGT]AGC
DraII 1791 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 1752 CCATGG C^C[GA][CT]GG
Eco81I 3189 CCTTAGG CC^T[ACGT]AGG
EcoO109I 1791 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 846 GAATTC G^AATTC
EcoT22I 2727 ATGCAT ATGCA^T
FauI 1741 GCGGG CCCGC(4/6)
HaeII 416 AGCGCC [GA]GCGC^[CT]
HincII 1677 GTTAAC GT[CT]^[GA]AC
HindII 1677 GTTAAC GT[CT]^[GA]AC
HpaI 1677 GTTAAC GTT^AAC
MamI 1213 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 908 AGATCC [GA]^GATC[CT]
MroI 1889 TCCGGA T^CCGGA
NcoI 1752 CCATGG C^CATGG
NsiI 2727 ATGCAT ATGCA^T
NspV 4120 TTCGAA TT^CGAA
PflFI 1117 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 2800 AGTACT AGT^ACT
SfuI 4120 TTCGAA TT^CGAA
SnaBI 4052 TACGTA TAC^GTA
Tth111I 1117 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 908 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 909 GATCC GGATC(4/5)
3579 GATCC
BbrPI 568 CACGTG CAC^GTG
2053 CACGTG
BseRI 751 CTCCTC GAGGAG(10/8)
3516 CTCCTC
BsgI 405 GTGCAG GTGCAG(16/14)
1208 GTGCAG
BsmFI 1579 GGGAC GGGAC(10/14)
1743 GGGAC
Bsp1407I 35 TGTACA T^GTACA
3027 TGTACA
BspMI 3726 GCAGGT ACCTGC(4/8)
3769 GCAGGT
BsrGI 35 TGTACA T^GTACA
3027 TGTACA
BstAPI 309 GCAAGTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1458 GCAGTACATGC
CfoI 417 GCGC GCG^C
1851 GCGC
HhaI 417 GCGC GCG^C
1851 GCGC
HinP1I 417 GCGC G^CGC
1851 GCGC
MspA1I 43 CAGCTG C[AC]G^C[GT]G
835 CAGCTG
PmaCI 568 CACGTG CAC^GTG
2053 CACGTG
PmlI 568 CACGTG CAC^GTG
2053 CACGTG
PstI 550 CTGCAG CTGCA^G
764 CTGCAG
PvuII 43 CAGCTG CAG^CTG
835 CAGCTG
SpeI 282 ACTAGT A^CTAGT
2423 ACTAGT
SspBI 35 TGTACA T^GTACA
3027 TGTACA
SwaI 2916 ATTTAAAT ATTT^AAAT
4189 ATTTAAAT
XbaI 26 TCTAGA T^CTAGA
1353 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwNI
ApoI AseI AsnI BbvI BfaI
BmyI BpmI BsaAI BsaJI BsaWI
BsiYI BslI BsmAI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I