##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AflII 2376 CTTAAG C^TTAAG
AflIII 1775 ACATGT A^C[GA][CT]GT
AlwNI 1058 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 604 CCTGAGG CC^T[ACGT]AGG
AspHI 501 GAGCAC G[AT]GC[AT]^C
BalI 2312 TGGCCA TGG^CCA
BamHI 851 GGATCC G^GATCC
BfrI 2376 CTTAAG C^TTAAG
BsaI 166 GGTCTC GGTCTC(1/5)
BsgI 637 CTGCAC GTGCAG(16/14)
BsiHKAI 501 GAGCAC G[AT]GC[AT]^C
BsmI 1439 GCATTC GAATGC(1/-1)
BspLU11I 1775 ACATGT A^CATGT
BspMI 2116 ACCTGC ACCTGC(4/8)
BstAPI 436 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1032 TTCGAA TT^CGAA
BstXI 1826 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 604 CCTGAGG CC^T[ACGT]AGG
CfoI 1923 GCGC GCG^C
DraI 1344 TTTAAA TTT^AAA
DraII 608 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 640 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2312 TGGCCA [CT]^GGCC[GA]
EarI 2127 GAAGAG CTCTTC(1/4)
Eco81I 604 CCTGAGG CC^T[ACGT]AGG
EcoNI 1758 CCTACTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 608 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 316 GATATC GAT^ATC
EcoT14I 2339 CCAAGG C^C[AT][AT]GG
HhaI 1923 GCGC GCG^C
HinP1I 1923 GCGC G^CGC
HindIII 1466 AAGCTT A^AGCTT
Ksp632I 2127 GAAGAG CTCTTC(1/4)
MluNI 2312 TGGCCA TGG^CCA
MscI 2312 TGGCCA TGG^CCA
NspV 1032 TTCGAA TT^CGAA
PflMI 339 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 633 GAGTC GAGTC(4/5)
PstI 1863 CTGCAG CTGCA^G
ScaI 2270 AGTACT AGT^ACT
SfuI 1032 TTCGAA TT^CGAA
StuI 962 AGGCCT AGG^CCT
StyI 2339 CCAAGG C^C[AT][AT]GG
TaqI 1033 TCGA T^CGA
TthHB8I 1033 TCGA T^CGA
Van91I 339 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1829 CCATGCTGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 1006 CCGC CCGC(-3/-1)
2266 CCGC
AseI 2389 ATTAAT AT^TAAT
2595 ATTAAT
AsnI 2389 ATTAAT AT^TAAT
2595 ATTAAT
BanI 1169 GGCACC G^G[CT][GA]CC
2180 GGTGCC
BbsI 598 GAAGAC GAAGAC(2/6)
1353 GTCTTC
BclI 1547 TGATCA T^GATCA
1554 TGATCA
BcnI 1737 CCCGG CC^[GC]GG
1919 CCCGG
BpmI 856 CTCCAG CTGGAG(16/14)
1966 CTCCAG
BpuAI 598 GAAGAC GAAGAC(2/6)
1353 GTCTTC
BsmFI 1628 GGGAC GGGAC(10/14)
363 GTCCC
BspHI 2067 TCATGA T^CATGA
2220 TCATGA
Cac8I 1709 GCTGGC GC[ACGT]^[ACGT]GC
2409 GCAAGC
EcoT22I 434 ATGCAT ATGCA^T
1976 ATGCAT
FbaI 1547 TGATCA T^GATCA
1554 TGATCA
HapII 1738 CCGG C^CGG
1920 CCGG
HpaII 1738 CCGG C^CGG
1920 CCGG
MspA1I 859 CAGCTG C[AC]G^C[GT]G
919 CAGCTG
MspI 1738 CCGG C^CGG
1920 CCGG
NciI 1737 CCCGG CC^[GC]GG
1919 CCCGG
NsiI 434 ATGCAT ATGCA^T
1976 ATGCAT
NspI 1082 ACATGC [GA]CATG^[CT]
1775 ACATGT
PshBI 2389 ATTAAT AT^TAAT
2595 ATTAAT
PvuII 859 CAGCTG CAG^CTG
919 CAGCTG
RcaI 2067 TCATGA T^CATGA
2220 TCATGA
SmlI 409 CTCAAG C^T[CT][GA]AG
2376 CTTAAG
SspI 2392 AATATT AAT^ATT
2443 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MunI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI