##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII BamHI
BbeI BbrPI BglI BsaAI BsaBI
BsaHI BsiWI BssHII BssSI Bst1107I
BstUI BstZ17I CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SphI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 3551 GTCTAC GT^[AC][GT]AC
AccIII 717 TCCGGA T^CCGGA
AgeI 1817 ACCGGT A^CCGGT
AocI 3689 CCTTAGG CC^T[ACGT]AGG
AspI 4058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3587 TGGCCA TGG^CCA
BanII 1110 GAGCCC G[GA]GC[CT]^C
BcgI 806 GCAGTGCGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 714 GTATCC GTATCC(6/5)
BlpI 1698 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1698 GCTAAGC GC^T[ACGT]AGC
BseAI 717 TCCGGA T^CCGGA
BsiEI 812 CGATCG CG[GA][CT]^CG
BspEI 717 TCCGGA T^CCGGA
BsrBI 621 GAGCGG CCGCTC(-3/-3)
BsrDI 1708 CATTGC GCAATG(2/0)
BstBI 2014 TTCGAA TT^CGAA
BstEII 450 GGTGACC G^GT[ACGT]ACC
BstPI 450 GGTGACC G^GT[ACGT]ACC
BstXI 4278 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3689 CCTTAGG CC^T[ACGT]AGG
CelII 1698 GCTAAGC GC^T[ACGT]AGC
DrdI 4058 GACTCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3689 CCTTAGG CC^T[ACGT]AGG
EcoO65I 450 GGTGACC G^GT[ACGT]ACC
EcoRI 2868 GAATTC G^AATTC
EcoRV 5345 GATATC GAT^ATC
HaeII 257 AGCGCC [GA]GCGC^[CT]
MluNI 3587 TGGCCA TGG^CCA
MroI 717 TCCGGA T^CCGGA
MscI 3587 TGGCCA TGG^CCA
MspA1I 1123 CAGCTG C[AC]G^C[GT]G
NaeI 221 GCCGGC GCC^GGC
NgoMIV 221 GCCGGC G^CCGGC
NspV 2014 TTCGAA TT^CGAA
PflFI 4058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1817 ACCGGT A^CCGGT
PvuI 812 CGATCG CGAT^CG
PvuII 1123 CAGCTG CAG^CTG
SfuI 2014 TTCGAA TT^CGAA
SmaI 183 CCCGGG CCC^GGG
SpeI 2255 ACTAGT A^CTAGT
StuI 3054 AGGCCT AGG^CCT
Tth111I 4058 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 183 CCCGGG C^CCGGG
XmaI 183 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 1083 CTTAAG C^TTAAG
3213 CTTAAG
AspHI 1597 GAGCAC G[AT]GC[AT]^C
3355 GTGCTC
AvaI 183 CCCGGG C^[CT]CG[GA]G
4145 CCCGAG
AvrII 3051 CCTAGG C^CTAGG
5531 CCTAGG
BanI 191 GGCACC G^G[CT][GA]CC
4165 GGTGCC
BbvCI 4136 CCTCAGC CCTCAGC(-5/-2)
4268 CCTCAGC
BclI 1900 TGATCA T^GATCA
3146 TGATCA
BfrI 1083 CTTAAG C^TTAAG
3213 CTTAAG
BglII 2019 AGATCT A^GATCT
3574 AGATCT
BlnI 3051 CCTAGG C^CTAGG
5531 CCTAGG
BseRI 46 GAGGAG GAGGAG(10/8)
284 GAGGAG
BsiHKAI 1597 GAGCAC G[AT]GC[AT]^C
3355 GTGCTC
BsmBI 158 GAGACG CGTCTC(1/5)
4208 GAGACG
BsmI 3536 GAATGC GAATGC(1/-1)
5244 GCATTC
BsoBI 183 CCCGGG C^[CT]CG[GA]G
4145 CCCGAG
BspDI 1416 ATCGAT AT^CGAT
1833 ATCGAT
BspHI 3623 TCATGA T^CATGA
5009 TCATGA
BsrFI 221 GCCGGC [GA]^CCGG[CT]
1817 ACCGGT
BstAPI 758 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4410 GCAAGTTGTGC
CfoI 258 GCGC GCG^C
4087 GCGC
Cfr10I 221 GCCGGC [GA]^CCGG[CT]
1817 ACCGGT
ClaI 1416 ATCGAT AT^CGAT
1833 ATCGAT
EcoT22I 1423 ATGCAT ATGCA^T
5457 ATGCAT
FbaI 1900 TGATCA T^GATCA
3146 TGATCA
HhaI 258 GCGC GCG^C
4087 GCGC
HinP1I 258 GCGC G^CGC
4087 GCGC
NsiI 1423 ATGCAT ATGCA^T
5457 ATGCAT
PflMI 900 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4107 CCACTTCCTGG
PstI 1396 CTGCAG CTGCA^G
1906 CTGCAG
RcaI 3623 TCATGA T^CATGA
5009 TCATGA
Van91I 900 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4107 CCACTTCCTGG
XcmI 2497 CCAAAGAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4782 CCAATATAGATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AvaII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI