##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AspI AviII AvrII BbeI
BbrPI BbsI BbvCI BciVI BcnI
BglI BglII BlnI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI Hin1I KasI
KspI MamI MluI MroI MspA1I
NaeI NarI NciI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I StuI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2557 GGTACC G^GTACC
AccI 369 GTAGAC GT^[AC][GT]AC
AccII 58 CGCG CG^CG
AocI 1360 CCTGAGG CC^T[ACGT]AGG
ApaI 142 GGGCCC GGGCC^C
AseI 1516 ATTAAT AT^TAAT
AsnI 1516 ATTAAT AT^TAAT
Asp718I 2557 GGTACC G^GTACC
AvaI 2052 CTCGGG C^[CT]CG[GA]G
BalI 2696 TGGCCA TGG^CCA
BanI 2557 GGTACC G^G[CT][GA]CC
BcgI 163 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1290 TGATCA T^GATCA
BsaAI 1269 TACGTG [CT]AC^GT[GA]
BsiYI 26 CCGCTCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 26 CCGCTCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1031 GAGAC GTCTC(1/5)
BsmBI 1031 GAGACG CGTCTC(1/5)
BsmI 1278 GAATGC GAATGC(1/-1)
BsoBI 2052 CTCGGG C^[CT]CG[GA]G
BsrDI 2930 GCAATG GCAATG(2/0)
BsrFI 129 GCCGGT [GA]^CCGG[CT]
BstUI 58 CGCG CG^CG
Bsu36I 1360 CCTGAGG CC^T[ACGT]AGG
Cfr10I 129 GCCGGT [GA]^CCGG[CT]
EaeI 2696 TGGCCA [CT]^GGCC[GA]
Eco81I 1360 CCTGAGG CC^T[ACGT]AGG
FauI 139 GCGGG CCCGC(4/6)
FbaI 1290 TGATCA T^GATCA
HaeII 117 AGCGCC [GA]GCGC^[CT]
HapII 130 CCGG C^CGG
HgaI 1282 GCGTC GACGC(5/10)
HindIII 237 AAGCTT A^AGCTT
HpaII 130 CCGG C^CGG
KpnI 2557 GGTACC GGTAC^C
MfeI 388 CAATTG C^AATTG
MluNI 2696 TGGCCA TGG^CCA
MscI 2696 TGGCCA TGG^CCA
MspI 130 CCGG C^CGG
MunI 388 CAATTG C^AATTG
MvnI 58 CGCG CG^CG
NdeI 825 CATATG CA^TATG
NheI 2573 GCTAGC G^CTAGC
PleI 2031 GACTC GAGTC(4/5)
PpuMI 1688 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1516 ATTAAT AT^TAAT
PstI 420 CTGCAG CTGCA^G
SgrAI 128 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 2160 ACTAGT A^CTAGT
SphI 1415 GCATGC GCATG^C
SwaI 2525 ATTTAAAT ATTT^AAAT
TfiI 1319 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 2368 CTTAAG C^TTAAG
2652 CTTAAG
AflIII 2247 ACATGT A^C[GA][CT]GT
3101 ACATGT
AlwNI 414 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1383 CAGGCCCTG
Asp700I 291 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
302 GAAGTTCTTC
BamHI 1749 GGATCC G^GATCC
2167 GGATCC
BanII 142 GGGCCC G[GA]GC[CT]^C
2326 GGGCTC
BfrI 2368 CTTAAG C^TTAAG
2652 CTTAAG
BseRI 1782 GAGGAG GAGGAG(10/8)
2352 GAGGAG
BsgI 1926 GTGCAG GTGCAG(16/14)
2413 GTGCAG
BsiEI 23 CGACCG CG[GA][CT]^CG
173 CGACCG
BspLU11I 2247 ACATGT A^CATGT
3101 ACATGT
BsrBI 26 CCGCTC CCGCTC(-3/-3)
49 CCGCTC
BstAPI 413 GCAGTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
930 GCACATTTTGC
CfoI 19 GCGC GCG^C
118 GCGC
HhaI 19 GCGC GCG^C
118 GCGC
HinP1I 19 GCGC G^CGC
118 GCGC
HinfI 1319 GATTC G^A[ACGT]TC
2031 GACTC
HpaI 1066 GTTAAC GTT^AAC
2943 GTTAAC
MslI 1294 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2969 CACGTTTATG
SfcI 420 CTGCAG C^T[GA][CT]AG
2925 CTGTAG
SspI 575 AATATT AAT^ATT
3241 AATATT
XmnI 291 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
302 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BsiHKAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
SspBI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII