##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI ApaI AscI AseI
AsnI Asp718I AspEI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI Bst1107I BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco52I Eco81I
EcoT22I FseI KpnI KspI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 3518 GAATTC [GA]^AATT[CT]
AflII 2879 CTTAAG C^TTAAG
ApoI 3518 GAATTC [GA]^AATT[CT]
AspI 2178 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1233 TGCGCA TGC^GCA
BcgI 621 GCACAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2138 GGATAC GTATCC(6/5)
BfrI 2879 CTTAAG C^TTAAG
BglI 1368 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 796 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3780 TCCGGT [AT]^CCGG[AT]
BseRI 1547 GAGGAG GAGGAG(10/8)
BsmI 2406 GCATTC GAATGC(1/-1)
BspLU11I 4208 ACATGT A^CATGT
BstXI 1827 CCAGTGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 4242 TTTAAA TTT^AAA
DrdI 1322 GACATCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3518 GAATTC G^AATTC
EcoRV 251 GATATC GAT^ATC
FspI 1233 TGCGCA TGC^GCA
HincII 3603 GTTAAC GT[CT]^[GA]AC
HindII 3603 GTTAAC GT[CT]^[GA]AC
HpaI 3603 GTTAAC GTT^AAC
MamI 796 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1428 CATATG CA^TATG
PflFI 2178 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 952 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Tth111I 2178 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1331 GTCTAC GT^[AC][GT]AC
4192 GTCTAC
BamHI 1 GGATCC G^GATCC
2481 GGATCC
BbeI 102 GGCGCC GGCGC^C
390 GGCGCC
BbrPI 1256 CACGTG CAC^GTG
1689 CACGTG
BbvCI 2433 CCTCAGC CCTCAGC(-5/-2)
2895 CCTCAGC
BclI 456 TGATCA T^GATCA
612 TGATCA
BglII 1197 AGATCT A^GATCT
1638 AGATCT
BlpI 289 GCTCAGC GC^T[ACGT]AGC
2959 GCTGAGC
Bpu1102I 289 GCTCAGC GC^T[ACGT]AGC
2959 GCTGAGC
BspHI 453 TCATGA T^CATGA
960 TCATGA
BspMI 340 GCAGGT ACCTGC(4/8)
1981 GCAGGT
BssHII 141 GCGCGC G^CGCGC
268 GCGCGC
BstEII 520 GGTGACC G^GT[ACGT]ACC
2101 GGTCACC
BstPI 520 GGTGACC G^GT[ACGT]ACC
2101 GGTCACC
CelII 289 GCTCAGC GC^T[ACGT]AGC
2959 GCTGAGC
EcoO65I 520 GGTGACC G^GT[ACGT]ACC
2101 GGTCACC
FbaI 456 TGATCA T^GATCA
612 TGATCA
HindIII 1723 AAGCTT A^AGCTT
2853 AAGCTT
KasI 102 GGCGCC G^GCGCC
390 GGCGCC
NarI 102 GGCGCC GG^CGCC
390 GGCGCC
PmaCI 1256 CACGTG CAC^GTG
1689 CACGTG
PmlI 1256 CACGTG CAC^GTG
1689 CACGTG
RcaI 453 TCATGA T^CATGA
960 TCATGA
SacI 1124 GAGCTC GAGCT^C
1406 GAGCTC
SapI 148 GCTCTTC GCTCTTC(1/4)
2204 GCTCTTC
SbfI 2345 CCTGCAGG CCTGCA^GG
2522 CCTGCAGG
SfoI 102 GGCGCC GGC^GCC
390 GGCGCC
Sse8387I 2345 CCTGCAGG CCTGCA^GG
2522 CCTGCAGG
StuI 1375 AGGCCT AGG^CCT
2527 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI Aor51HI
ApaLI Asp700I AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvI BcnI BfaI BlnI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI