##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BglII BlnI
BsiWI BspDI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
Eam1105I Eco47III Eco81I FspI HpaI
KpnI KspI MluI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PvuI RsrII
SacII SapI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1753 GACGTC GACGT^C
AccI 3791 GTCGAC GT^[AC][GT]AC
AccIII 2319 TCCGGA T^CCGGA
BamHI 50 GGATCC G^GATCC
BanI 1497 GGCGCC G^G[CT][GA]CC
BbeI 1497 GGCGCC GGCGC^C
BbrPI 2803 CACGTG CAC^GTG
BbsI 2911 GTCTTC GAAGAC(2/6)
BcgI 882 CGAGGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2074 GTATCC GTATCC(6/5)
BlpI 2047 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2047 GCTGAGC GC^T[ACGT]AGC
BpuAI 2911 GTCTTC GAAGAC(2/6)
BseAI 2319 TCCGGA T^CCGGA
BsmI 3389 GAATGC GAATGC(1/-1)
Bsp1407I 4476 TGTACA T^GTACA
BspEI 2319 TCCGGA T^CCGGA
BspLU11I 198 ACATGT A^CATGT
BspMI 4373 GCAGGT ACCTGC(4/8)
BsrBI 1491 GAGCGG CCGCTC(-3/-3)
BsrGI 4476 TGTACA T^GTACA
BssSI 1513 CACGAG CACGAG(-5/-1)
BstAPI 2659 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2423 GGTCACC G^GT[ACGT]ACC
BstPI 2423 GGTCACC G^GT[ACGT]ACC
CelII 2047 GCTGAGC GC^T[ACGT]AGC
DrdI 712 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1257 CCTGGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2423 GGTCACC G^GT[ACGT]ACC
EcoRI 282 GAATTC G^AATTC
EcoT22I 937 ATGCAT ATGCA^T
FseI 1456 GGCCGGCC GGCCGG^CC
KasI 1497 GGCGCC G^GCGCC
MfeI 2951 CAATTG C^AATTG
MroI 2319 TCCGGA T^CCGGA
MunI 2951 CAATTG C^AATTG
NaeI 1457 GCCGGC GCC^GGC
NarI 1497 GGCGCC GG^CGCC
NgoMIV 1457 GCCGGC G^CCGGC
NsiI 937 ATGCAT ATGCA^T
PmaCI 2803 CACGTG CAC^GTG
PmlI 2803 CACGTG CAC^GTG
PstI 2612 CTGCAG CTGCA^G
SacI 442 GAGCTC GAGCT^C
SalI 3791 GTCGAC G^TCGAC
SexAI 1353 ACCTGGT A^CC[AT]GGT
SfoI 1497 GGCGCC GGC^GCC
SphI 2013 GCATGC GCATG^C
SspBI 4476 TGTACA T^GTACA
SspI 3994 AATATT AAT^ATT
XcmI 1778 CCAAATACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AclI 3223 AACGTT AA^CGTT
4198 AACGTT
AflII 1024 CTTAAG C^TTAAG
2935 CTTAAG
AflIII 198 ACATGT A^C[GA][CT]GT
2773 ACGTGT
AseI 3371 ATTAAT AT^TAAT
4426 ATTAAT
AsnI 3371 ATTAAT AT^TAAT
4426 ATTAAT
BalI 1472 TGGCCA TGG^CCA
3748 TGGCCA
BbvCI 1894 GCTGAGG CCTCAGC(-5/-2)
4417 GCTGAGG
BclI 2444 TGATCA T^GATCA
4262 TGATCA
BfrI 1024 CTTAAG C^TTAAG
2935 CTTAAG
BglI 892 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1383 GCCCTTGGGGC
BpmI 586 CTCCAG CTGGAG(16/14)
2705 CTCCAG
BsaAI 2803 CACGTG [CT]AC^GT[GA]
2922 CACGTA
BsaBI 1366 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3879 GATTTTGATC
BsaI 1252 GAGACC GGTCTC(1/5)
2283 GAGACC
BsaWI 2319 TCCGGA [AT]^CCGG[AT]
3042 TCCGGT
BsmBI 1755 CGTCTC CGTCTC(1/5)
2515 GAGACG
BspHI 278 TCATGA T^CATGA
990 TCATGA
BsrDI 2816 CATTGC GCAATG(2/0)
4090 CATTGC
BsrFI 1457 GCCGGC [GA]^CCGG[CT]
2652 ACCGGC
BstXI 2130 CCAGCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3250 CCAGATCCATGG
Cfr10I 1457 GCCGGC [GA]^CCGG[CT]
2652 ACCGGC
EarI 913 CTCTTC CTCTTC(1/4)
3351 GAAGAG
FbaI 2444 TGATCA T^GATCA
4262 TGATCA
Ksp632I 913 CTCTTC CTCTTC(1/4)
3351 GAAGAG
MamI 1366 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3879 GATTTTGATC
MluNI 1472 TGGCCA TGG^CCA
3748 TGGCCA
MscI 1472 TGGCCA TGG^CCA
3748 TGGCCA
NheI 2154 GCTAGC G^CTAGC
2545 GCTAGC
PshBI 3371 ATTAAT AT^TAAT
4426 ATTAAT
Psp1406I 3223 AACGTT AA^CGTT
4198 AACGTT
PvuII 1679 CAGCTG CAG^CTG
3409 CAGCTG
RcaI 278 TCATGA T^CATGA
990 TCATGA
ScaI 3115 AGTACT AGT^ACT
3649 AGTACT
SmaI 31 CCCGGG CCC^GGG
140 CCCGGG
XmaCI 31 CCCGGG C^CCGGG
140 CCCGGG
XmaI 31 CCCGGG C^CCGGG
140 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI