##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BfrI BlnI BsaAI BsgI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI BstAPI
BstBI Bsu36I ClaI CpoI DrdI
Eco47III Eco81I EcoRV FseI FspI
HaeII HincII HindII HpaI KasI
KpnI MfeI MluI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI SexAI SfoI SfuI SmlI
SnaBI SpeI Sse8387I SspBI SwaI
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 2173 GTATAC GT^[AC][GT]AC
AccIII 164 TCCGGA T^CCGGA
AcyI 105 GGCGTC G[GA]^CG[CT]C
AgeI 1772 ACCGGT A^CCGGT
AhdI 1761 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2265 GTGCAC G^TGCAC
ApaLI 2265 GTGCAC G^TGCAC
Asp700I 1008 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1761 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2465 GGATCC G^GATCC
BanII 700 GAGCTC G[GA]GC[CT]^C
BlpI 237 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 237 GCTCAGC GC^T[ACGT]AGC
BsaBI 502 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 105 GGCGTC G[GA]^CG[CT]C
BseAI 164 TCCGGA T^CCGGA
BsiEI 217 CGGCCG CG[GA][CT]^CG
BsmI 1482 GCATTC GAATGC(1/-1)
BspEI 164 TCCGGA T^CCGGA
BspHI 980 TCATGA T^CATGA
BsrDI 776 GCAATG GCAATG(2/0)
Bst1107I 2173 GTATAC GTA^TAC
BstZ17I 2173 GTATAC GTA^TAC
CelII 237 GCTCAGC GC^T[ACGT]AGC
DraIII 1134 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 217 CGGCCG C^GGCCG
Eam1105I 1761 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 217 CGGCCG C^GGCCG
Eco52I 217 CGGCCG C^GGCCG
EcoRI 1070 GAATTC G^AATTC
FauI 96 GCGGG CCCGC(4/6)
Hin1I 105 GGCGTC G[GA]^CG[CT]C
HindIII 846 AAGCTT A^AGCTT
KspI 78 CCGCGG CCGC^GG
MamI 502 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 164 TCCGGA T^CCGGA
MslI 348 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 910 GCTAGC G^CTAGC
PflFI 2367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1772 ACCGGT A^CCGGT
PpuMI 44 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 895 CTGCAG CTGCA^G
PvuII 994 CAGCTG CAG^CTG
RcaI 980 TCATGA T^CATGA
SacI 700 GAGCTC GAGCT^C
SacII 78 CCGCGG CCGC^GG
SapI 702 GCTCTTC GCTCTTC(1/4)
ScaI 1112 AGTACT AGT^ACT
SfiI 630 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1771 CACCGGTG C[GA]^CCGG[CT]G
SmaI 40 CCCGGG CCC^GGG
SphI 1437 GCATGC GCATG^C
SspI 2238 AATATT AAT^ATT
StuI 638 AGGCCT AGG^CCT
Tth111I 2367 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1378 CCAGAGATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 40 CCCGGG C^CCGGG
XmaI 40 CCCGGG C^CCGGG
XmnI 1008 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 40 CCCGGG C^[CT]CG[GA]G
57 CTCGGG
BanI 1366 GGCACC G^G[CT][GA]CC
2322 GGTGCC
BbsI 298 GAAGAC GAAGAC(2/6)
1420 GAAGAC
BglII 1221 AGATCT A^GATCT
1382 AGATCT
BpuAI 298 GAAGAC GAAGAC(2/6)
1420 GAAGAC
BsaI 661 GGTCTC GGTCTC(1/5)
1738 GGTCTC
BsaWI 164 TCCGGA [AT]^CCGG[AT]
1772 ACCGGT
BseRI 718 CTCCTC GAGGAG(10/8)
1601 GAGGAG
BsoBI 40 CCCGGG C^[CT]CG[GA]G
57 CTCGGG
BspMI 2279 ACCTGC ACCTGC(4/8)
474 GCAGGT
DraI 2475 TTTAAA TTT^AAA
2495 TTTAAA
DraII 44 GGGTCCC [GA]G^G[ACGT]CC[CT]
1719 GGGGCCT
EaeI 217 CGGCCG [CT]^GGCC[GA]
1691 TGGCCG
EcoO109I 44 GGGTCCC [GA]G^G[ACGT]CC[CT]
1719 GGGGCCT
EcoT22I 157 ATGCAT ATGCA^T
712 ATGCAT
HgaI 106 GCGTC GACGC(5/10)
1696 GCGTC
MaeII 1732 ACGT A^CGT
2456 ACGT
NsiI 157 ATGCAT ATGCA^T
712 ATGCAT
NspI 1437 GCATGC [GA]CATG^[CT]
1762 ACATGC
SfcI 895 CTGCAG C^T[GA][CT]AG
1371 CTACAG
XbaI 2092 TCTAGA T^CTAGA
2428 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BclI BcnI BfaI BglI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EarI EcoNI EcoO65I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII