HUGE

Gene/Protein Characteristic Table for KIAA0027

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00373
Accession No. :	D25217
Description :	Membrane protein MLC1.
HUGO Gene Name :	megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1)
Clone Name :	fk03155 [Vector Info]
Flexi ORF Clone :	pF1KSDA0027
Source :	Human fetal brain
Note :	We replaced ha00522, former representative clones for KIAA0027 with fk03155. (2002/5/10)

Features of the cloned DNA sequence	Description

Length: 3629 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 2185 bp

Genome contig ID gi89161203r_48739954
PolyA signal sequence
(AATAAA,-17)
+----*----+----*----+----*----+----
TGTTTAATACGCTTGAGCAATAAACGCTGACTTGC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGACGTGGCTGTGGCTATTTAATATTGTGTCTCAGCAGCGTCAGCTCACT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 22 r 48839954 48866161 12 99.4 Perfect prediction

Features of the protein sequence	Description

Length: 418 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
AAH70042	1.5e-155	99.7	Megalencephalic...	Homo sapiens
AAH28425	1.5e-155	99.7	Megalencephalic...	Homo sapiens
Q60HE7	7.5e-152	96.8	Membrane protei...	Macaca fascicularis
EDL04387	1e-139	85.7	megalencephalic...	Mus musculus
EDL76527	5.7e-138	85.0	megalencephalic...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

ScanRegExp IPR001209 125 147 PS00527 Ribosomal protein S14

Search method	interpro_ID	From	To	Entry	Definition
ScanRegExp	IPR001209	125	147	PS00527	Ribosomal protein S14

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 94 VFSVLMGSCLLVTSGFSLYLGNV 116 PRIMARY 23

2 121 MDYLRCAAGSCIPSAIVSFTVS 142 SECONDARY 22

3 151 NFQILFVSTFAVTTTCLIWFGCK 173 PRIMARY 23

4 184 NFNLILLLLLELLMAATVIIAAR 206 PRIMARY 23

5 240 SVVEVIAGISAVLGGIIALNVDD 262 PRIMARY 23

6 268 HLSVTFFWILVACFPSAIASHVA 290 PRIMARY 23

7 302 LIAISSLTSPLLFTASGYLSFSI 324 PRIMARY 23

8 338 IKPSYDVLLLLLLLVLLLQAGLN 360 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	94	VFSVLMGSCLLVTSGFSLYLGNV	116	PRIMARY	23
2	121	MDYLRCAAGSCIPSAIVSFTVS	142	SECONDARY	22
3	151	NFQILFVSTFAVTTTCLIWFGCK	173	PRIMARY	23
4	184	NFNLILLLLLELLMAATVIIAAR	206	PRIMARY	23
5	240	SVVEVIAGISAVLGGIIALNVDD	262	PRIMARY	23
6	268	HLSVTFFWILVACFPSAIASHVA	290	PRIMARY	23
7	302	LIAISSLTSPLLFTASGYLSFSI	324	PRIMARY	23
8	338	IKPSYDVLLLLLLLVLLLQAGLN	360	PRIMARY	23

Expression profile	Description

: Northern blot Description
: RT-PCR-ELISA Description

Northern blot	Description

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 22

Experimental conditions

Panel name	: Genebridge 4
Primer_f	: TCAGGGGCCGGGCAAACACT
Primer_r	: CTCAAAGCTGCAGGGAGGTG
PCR product length	: 363 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	YES

Length of 3'UTR	2185 bp
Genome contig ID	gi89161203r_48739954
PolyA signal sequence (AATAAA,-17)	+--------+--------+----*----+---- TGTTTAATACGCTTGAGCAATAAACGCTGACTTGC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGACGTGGCTGTGGCTATTTAATATTGTGTCTCAGCAGCGTCAGCTCACT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	22	r	48839954	48866161	12	99.4	Perfect prediction

Gene/Protein Characteristic Table for KIAA0027

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information