##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AflII AgeI AocI AscI
AseI AsnI AviII BbvCI BciVI
BfrI BglII BsaBI BsiEI BsiWI
BspDI BspLU11I BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DrdI EagI EclXI Eco52I Eco81I
FspI HincII HindII HindIII HpaI
KspI MamI MluI NdeI NheI
NotI NruI NspI NspV PacI
PaeR7I PinAI PmeI PshBI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3312 GGTACC G^GTACC
AccI 3584 GTCTAC GT^[AC][GT]AC
AclI 2880 AACGTT AA^CGTT
AcsI 2489 GAATTC [GA]^AATT[CT]
AhdI 1823 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1937 GTGCAC G^TGCAC
Aor51HI 3534 AGCGCT AGC^GCT
ApaLI 1937 GTGCAC G^TGCAC
ApoI 2489 GAATTC [GA]^AATT[CT]
Asp718I 3312 GGTACC G^GTACC
AspEI 1823 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1590 CCTAGG C^CTAGG
BclI 789 TGATCA T^GATCA
BlnI 1590 CCTAGG C^CTAGG
BsaI 216 GAGACC GGTCTC(1/5)
BsgI 798 CTGCAC GTGCAG(16/14)
BsmI 2793 GAATGC GAATGC(1/-1)
Bsp1407I 275 TGTACA T^GTACA
BsrBI 365 GAGCGG CCGCTC(-3/-3)
BsrGI 275 TGTACA T^GTACA
BstAPI 3022 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 499 GGTGACC G^GT[ACGT]ACC
BstPI 499 GGTGACC G^GT[ACGT]ACC
DraIII 676 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1823 GACTGTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3534 AGCGCT AGC^GCT
EcoO65I 499 GGTGACC G^GT[ACGT]ACC
EcoRI 2489 GAATTC G^AATTC
EcoRV 1140 GATATC GAT^ATC
EcoT22I 2948 ATGCAT ATGCA^T
FbaI 789 TGATCA T^GATCA
KpnI 3312 GGTACC GGTAC^C
MfeI 591 CAATTG C^AATTG
MunI 591 CAATTG C^AATTG
NsiI 2948 ATGCAT ATGCA^T
PshAI 460 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2880 AACGTT AA^CGTT
SacI 3457 GAGCTC GAGCT^C
SapI 1969 GCTCTTC GCTCTTC(1/4)
ScaI 2530 AGTACT AGT^ACT
SpeI 706 ACTAGT A^CTAGT
SspBI 275 TGTACA T^GTACA
StuI 2989 AGGCCT AGG^CCT
XbaI 2146 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 403 CGCG CG^CG
1453 CGCG
AccIII 3223 TCCGGA T^CCGGA
3547 TCCGGA
AcyI 1998 GGCGCC G[GA]^CG[CT]C
2729 GGCGCC
BamHI 1011 GGATCC G^GATCC
2153 GGATCC
BbeI 1998 GGCGCC GGCGC^C
2729 GGCGCC
BcgI 411 CGAGCGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
429 CGAAGAGACTGC
BglI 2021 GCCGCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2162 GCCACACTGGC
BlpI 164 GCTGAGC GC^T[ACGT]AGC
2421 GCTGAGC
Bpu1102I 164 GCTGAGC GC^T[ACGT]AGC
2421 GCTGAGC
BsaHI 1998 GGCGCC G[GA]^CG[CT]C
2729 GGCGCC
BseAI 3223 TCCGGA T^CCGGA
3547 TCCGGA
BspEI 3223 TCCGGA T^CCGGA
3547 TCCGGA
BspHI 1158 TCATGA T^CATGA
3111 TCATGA
BsrDI 952 CATTGC GCAATG(2/0)
1039 CATTGC
BstUI 403 CGCG CG^CG
1453 CGCG
BstXI 855 CCAACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1044 CCAGTCATGTGG
CelII 164 GCTGAGC GC^T[ACGT]AGC
2421 GCTGAGC
DraI 1606 TTTAAA TTT^AAA
3562 TTTAAA
EcoNI 1246 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1942 CCTGCCCCAGG
FseI 1255 GGCCGGCC GGCCGG^CC
2170 GGCCGGCC
Hin1I 1998 GGCGCC G[GA]^CG[CT]C
2729 GGCGCC
KasI 1998 GGCGCC G^GCGCC
2729 GGCGCC
MroI 3223 TCCGGA T^CCGGA
3547 TCCGGA
MvnI 403 CGCG CG^CG
1453 CGCG
NarI 1998 GGCGCC GG^CGCC
2729 GGCGCC
NcoI 2687 CCATGG C^CATGG
2922 CCATGG
RcaI 1158 TCATGA T^CATGA
3111 TCATGA
SfoI 1998 GGCGCC GGC^GCC
2729 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI