##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AviII AvrII
BbsI BcgI BfrI BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrFI BssHII BstBI CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FauI FseI FspI
KpnI KspI MamI MfeI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 13 GGCGCC G[GA]^CG[CT]C
ApaI 3788 GGGCCC GGGCC^C
AseI 4034 ATTAAT AT^TAAT
AsnI 4034 ATTAAT AT^TAAT
AspHI 38 GTGCTC G[AT]GC[AT]^C
AspI 2775 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3612 GGATCC G^GATCC
BbeI 13 GGCGCC GGCGC^C
BbrPI 1116 CACGTG CAC^GTG
BciVI 1745 GGATAC GTATCC(6/5)
BglII 2578 AGATCT A^GATCT
BsaHI 13 GGCGCC G[GA]^CG[CT]C
BsaI 3386 GGTCTC GGTCTC(1/5)
BsaWI 3895 TCCGGT [AT]^CCGG[AT]
BsgI 474 CTGCAC GTGCAG(16/14)
BsiHKAI 38 GTGCTC G[AT]GC[AT]^C
BsmBI 1495 GAGACG CGTCTC(1/5)
Bsp1407I 1852 TGTACA T^GTACA
BspLU11I 4132 ACATGT A^CATGT
BsrGI 1852 TGTACA T^GTACA
BssSI 2919 CACGAG CACGAG(-5/-1)
BstEII 2399 GGTGACC G^GT[ACGT]ACC
BstPI 2399 GGTGACC G^GT[ACGT]ACC
EcoO65I 2399 GGTGACC G^GT[ACGT]ACC
EcoRV 2208 GATATC GAT^ATC
Hin1I 13 GGCGCC G[GA]^CG[CT]C
HindIII 2728 AAGCTT A^AGCTT
KasI 13 GGCGCC G^GCGCC
MluI 946 ACGCGT A^CGCGT
NarI 13 GGCGCC GG^CGCC
PaeR7I 3236 CTCGAG C^TCGAG
PflFI 2775 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1116 CACGTG CAC^GTG
PmlI 1116 CACGTG CAC^GTG
PshBI 4034 ATTAAT AT^TAAT
SapI 3843 GAAGAGC GCTCTTC(1/4)
ScaI 1317 AGTACT AGT^ACT
SfoI 13 GGCGCC GGC^GCC
SphI 985 GCATGC GCATG^C
SspBI 1852 TGTACA T^GTACA
Tth111I 2775 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3236 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 947 CGCG CG^CG
1637 CGCG
BbvCI 2325 CCTCAGC CCTCAGC(-5/-2)
2352 GCTGAGG
BclI 1922 TGATCA T^GATCA
2716 TGATCA
BglI 1077 GCCTATACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3580 GCCTCCCAGGC
Bst1107I 335 GTATAC GTA^TAC
3678 GTATAC
BstUI 947 CGCG CG^CG
1637 CGCG
BstZ17I 335 GTATAC GTA^TAC
3678 GTATAC
CfoI 14 GCGC GCG^C
2595 GCGC
EcoNI 1699 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1976 CCTTATCAAGG
EcoRI 1309 GAATTC G^AATTC
2432 GAATTC
FbaI 1922 TGATCA T^GATCA
2716 TGATCA
HaeII 13 GGCGCC [GA]GCGC^[CT]
2594 GGCGCT
HhaI 14 GCGC GCG^C
2595 GCGC
HinP1I 14 GCGC G^CGC
2595 GCGC
HpaI 798 GTTAAC GTT^AAC
4158 GTTAAC
MvnI 947 CGCG CG^CG
1637 CGCG
PpuMI 805 GGGACCC [GA]G^G[AT]CC[CT]
1249 AGGTCCC
SmaI 1474 CCCGGG CCC^GGG
2764 CCCGGG
SmlI 1175 CTTGAG C^T[CT][GA]AG
3236 CTCGAG
SspI 642 AATATT AAT^ATT
4037 AATATT
XmaCI 1474 CCCGGG C^CCGGG
2764 CCCGGG
XmaI 1474 CCCGGG C^CCGGG
2764 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI