##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI BamHI BbrPI
BbvCI BcgI BglI BsaBI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoNI FseI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SbfI SfiI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 2612 GTAGAC GT^[AC][GT]AC
AclI 1005 AACGTT AA^CGTT
AflII 4101 CTTAAG C^TTAAG
AlwNI 2801 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4066 CCTAAGG CC^T[ACGT]AGG
ApaI 3866 GGGCCC GGGCC^C
AspHI 1880 GTGCTC G[AT]GC[AT]^C
AvaI 2765 CCCGGG C^[CT]CG[GA]G
AviII 2032 TGCGCA TGC^GCA
BbsI 992 GAAGAC GAAGAC(2/6)
BciVI 2785 GTATCC GTATCC(6/5)
BfrI 4101 CTTAAG C^TTAAG
BlpI 2684 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2684 GCTCAGC GC^T[ACGT]AGC
BpuAI 992 GAAGAC GAAGAC(2/6)
BsaAI 813 CACGTA [CT]AC^GT[GA]
BsaWI 3042 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1880 GTGCTC G[AT]GC[AT]^C
BsoBI 2765 CCCGGG C^[CT]CG[GA]G
BspHI 1749 TCATGA T^CATGA
BsrDI 4160 CATTGC GCAATG(2/0)
BsrFI 11 GCCGGT [GA]^CCGG[CT]
BstAPI 3191 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2363 GGTTACC G^GT[ACGT]ACC
BstPI 2363 GGTTACC G^GT[ACGT]ACC
Bsu36I 4066 CCTAAGG CC^T[ACGT]AGG
CelII 2684 GCTCAGC GC^T[ACGT]AGC
Cfr10I 11 GCCGGT [GA]^CCGG[CT]
DraI 3023 TTTAAA TTT^AAA
DrdI 2615 GACAATATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 135 CCACGG C^C[GA][CT]GG
Eco81I 4066 CCTAAGG CC^T[ACGT]AGG
EcoO65I 2363 GGTTACC G^GT[ACGT]ACC
EcoRV 1845 GATATC GAT^ATC
FspI 2032 TGCGCA TGC^GCA
HgaI 333 GACGC GACGC(5/10)
HindIII 3005 AAGCTT A^AGCTT
PpuMI 3054 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1005 AACGTT AA^CGTT
PstI 2807 CTGCAG CTGCA^G
RcaI 1749 TCATGA T^CATGA
ScaI 1308 AGTACT AGT^ACT
SmaI 2765 CCCGGG CCC^GGG
XbaI 2750 TCTAGA T^CTAGA
XmaCI 2765 CCCGGG C^CCGGG
XmaI 2765 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 1227 GGCGCC G[GA]^CG[CT]C
3032 GGCGCC
AhdI 2754 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3056 GACCCCCAGTC
AspEI 2754 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3056 GACCCCCAGTC
AvrII 1627 CCTAGG C^CTAGG
3395 CCTAGG
BalI 2128 TGGCCA TGG^CCA
2797 TGGCCA
BanI 1227 GGCGCC G^G[CT][GA]CC
3032 GGCGCC
BanII 3783 GAGCCC G[GA]GC[CT]^C
3866 GGGCCC
BbeI 1227 GGCGCC GGCGC^C
3032 GGCGCC
BglII 703 AGATCT A^GATCT
1265 AGATCT
BlnI 1627 CCTAGG C^CTAGG
3395 CCTAGG
BsaHI 1227 GGCGCC G[GA]^CG[CT]C
3032 GGCGCC
BsaI 1131 GAGACC GGTCTC(1/5)
1632 GGTCTC
BsgI 2831 CTGCAC GTGCAG(16/14)
3189 CTGCAC
BstBI 363 TTCGAA TT^CGAA
1356 TTCGAA
DraIII 2472 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3680 CACAATGTG
EaeI 2128 TGGCCA [CT]^GGCC[GA]
2797 TGGCCA
Eam1105I 2754 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3056 GACCCCCAGTC
EcoRI 360 GAATTC G^AATTC
2226 GAATTC
EcoT22I 3464 ATGCAT ATGCA^T
4075 ATGCAT
HaeII 1227 GGCGCC [GA]GCGC^[CT]
3032 GGCGCC
Hin1I 1227 GGCGCC G[GA]^CG[CT]C
3032 GGCGCC
KasI 1227 GGCGCC G^GCGCC
3032 GGCGCC
MluNI 2128 TGGCCA TGG^CCA
2797 TGGCCA
MscI 2128 TGGCCA TGG^CCA
2797 TGGCCA
MslI 3495 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3538 CATTCAAATG
NarI 1227 GGCGCC GG^CGCC
3032 GGCGCC
NsiI 3464 ATGCAT ATGCA^T
4075 ATGCAT
NspV 363 TTCGAA TT^CGAA
1356 TTCGAA
PflMI 2471 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2789 CCATTACTTGG
SapI 3780 GAAGAGC GCTCTTC(1/4)
2352 GCTCTTC
SfoI 1227 GGCGCC GGC^GCC
3032 GGCGCC
SfuI 363 TTCGAA TT^CGAA
1356 TTCGAA
Van91I 2471 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2789 CCATTACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaII BbvI
BclI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI