##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspHI
AvaII AviII AvrII BamHI BbrPI
BbsI BcgI BciVI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaWI BseAI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstPI BstYI CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI FseI FspI
HindIII KpnI KspI MfeI MflI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AhdI 211 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3117 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2266 CCTCAGG CC^T[ACGT]AGG
AspEI 211 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 7 CTCGGG C^[CT]CG[GA]G
BalI 1938 TGGCCA TGG^CCA
BanII 2392 GGGCTC G[GA]GC[CT]^C
BbvCI 359 CCTCAGC CCTCAGC(-5/-2)
BclI 2707 TGATCA T^GATCA
BcnI 2243 CCGGG CC^[GC]GG
BsaBI 1902 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 14 GAGGAG GAGGAG(10/8)
BsgI 1531 GTGCAG GTGCAG(16/14)
BsiEI 158 CGATCG CG[GA][CT]^CG
BsoBI 7 CTCGGG C^[CT]CG[GA]G
BsrDI 1553 CATTGC GCAATG(2/0)
BssSI 1713 CTCGTG CACGAG(-5/-1)
Bsu36I 2266 CCTCAGG CC^T[ACGT]AGG
Eam1105I 211 GACGCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1090 CTCTTC CTCTTC(1/4)
Eco81I 2266 CCTCAGG CC^T[ACGT]AGG
EcoRV 1852 GATATC GAT^ATC
FbaI 2707 TGATCA T^GATCA
HgaI 211 GACGC GACGC(5/10)
HincII 2988 GTTAAC GT[CT]^[GA]AC
HindII 2988 GTTAAC GT[CT]^[GA]AC
HpaI 2988 GTTAAC GTT^AAC
Ksp632I 1090 CTCTTC CTCTTC(1/4)
MamI 1902 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1938 TGGCCA TGG^CCA
MscI 1938 TGGCCA TGG^CCA
MslI 1153 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 204 CAGCGG C[AC]G^C[GT]G
NciI 2243 CCGGG CC^[GC]GG
NcoI 132 CCATGG C^CATGG
NdeI 1989 CATATG CA^TATG
NspI 1822 GCATGT [GA]CATG^[CT]
PflMI 1564 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 321 CTGCAG CTGCA^G
PvuI 158 CGATCG CGAT^CG
SwaI 2728 ATTTAAAT ATTT^AAAT
Van91I 1564 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 226 GGCGCC G[GA]^CG[CT]C
1622 GGCGCC
AlwI 1303 GATCC GGATC(4/5)
275 GGATC
AspI 1500 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2054 GACGGAGTC
BbeI 226 GGCGCC GGCGC^C
1622 GGCGCC
BglI 863 GCCATTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1516 GCCGCCCAGGC
BmyI 2326 GTGCCC G[AGT]GC[ACT]^C
2392 GGGCTC
BpmI 1526 CTGGAG CTGGAG(16/14)
2078 CTGGAG
BsaHI 226 GGCGCC G[GA]^CG[CT]C
1622 GGCGCC
Bsp1286I 2326 GTGCCC G[AGT]GC[ACT]^C
2392 GGGCTC
BspMI 697 GCAGGT ACCTGC(4/8)
1396 GCAGGT
BsrBI 109 CCGCTC CCGCTC(-3/-3)
1548 CCGCTC
Cfr13I 1776 GGCCC G^G[ACGT]CC
2148 GGCCC
EaeI 1938 TGGCCA [CT]^GGCC[GA]
2240 TGGCCG
EcoT22I 1063 ATGCAT ATGCA^T
2577 ATGCAT
HapII 26 CCGG C^CGG
2243 CCGG
Hin1I 226 GGCGCC G[GA]^CG[CT]C
1622 GGCGCC
HpaII 26 CCGG C^CGG
2243 CCGG
KasI 226 GGCGCC G^GCGCC
1622 GGCGCC
MaeII 706 ACGT A^CGT
801 ACGT
MspI 26 CCGG C^CGG
2243 CCGG
NarI 226 GGCGCC GG^CGCC
1622 GGCGCC
NsiI 1063 ATGCAT ATGCA^T
2577 ATGCAT
PflFI 1500 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2054 GACGGAGTC
Sau96I 1776 GGCCC G^G[ACGT]CC
2148 GGCCC
SfoI 226 GGCGCC GGC^GCC
1622 GGCGCC
TaqI 1700 TCGA T^CGA
1856 TCGA
TfiI 2139 GATTC G^A[AT]TC
2790 GATTC
Tth111I 1500 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2054 GACGGAGTC
TthHB8I 1700 TCGA T^CGA
1856 TCGA
XbaI 1373 TCTAGA T^CTAGA
1898 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI ApoI AseI AsnI BanI
BbvI BfaI BsaI BsaJI BsiYI
BslI BsmAI BsmBI BsmFI BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstXI BstZ17I Cac8I CfoI DdeI
DpnII DraI DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PshBI RsaI
Sau3AI ScaI ScrFI SfaNI SfcI
SspI StyI Tru9I TseI Tsp45I
Tsp509I TspRI