##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AcyI AgeI AhdI ApaI
AscI AspEI AviII AvrII BbeI
BcgI BglI BglII BlnI BsaHI
BsiEI BsiWI BsmBI BspLU11I BsrBI
BssHII Bst1107I BstZ17I CpoI EagI
Eam1105I EclXI Eco52I FseI FspI
Hin1I HindIII KasI KspI MfeI
MluI MunI NaeI NarI NgoMIV
NotI NruI PacI PinAI PmeI
PshAI PvuI RsrII SacII SbfI
SfiI SfoI SgrAI Sse8387I
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1040 GGTACC G^GTACC
AccI 2601 GTCGAC GT^[AC][GT]AC
AccIII 3387 TCCGGA T^CCGGA
AflII 2459 CTTAAG C^TTAAG
Alw44I 2351 GTGCAC G^TGCAC
Aor51HI 5206 AGCGCT AGC^GCT
ApaLI 2351 GTGCAC G^TGCAC
AseI 2963 ATTAAT AT^TAAT
AsnI 2963 ATTAAT AT^TAAT
Asp718I 1040 GGTACC G^GTACC
AspI 2516 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2941 TGGCCA TGG^CCA
BbrPI 3285 CACGTG CAC^GTG
BbvCI 2711 GCTGAGG CCTCAGC(-5/-2)
BclI 4068 TGATCA T^GATCA
BfrI 2459 CTTAAG C^TTAAG
BsaWI 3387 TCCGGA [AT]^CCGG[AT]
BseAI 3387 TCCGGA T^CCGGA
BsmI 1862 GCATTC GAATGC(1/-1)
BspDI 2508 ATCGAT AT^CGAT
BspEI 3387 TCCGGA T^CCGGA
BsrFI 1244 GCCGGT [GA]^CCGG[CT]
BssSI 4470 CTCGTG CACGAG(-5/-1)
BstAPI 116 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3776 GGTGACC G^GT[ACGT]ACC
BstPI 3776 GGTGACC G^GT[ACGT]ACC
Cfr10I 1244 GCCGGT [GA]^CCGG[CT]
ClaI 2508 ATCGAT AT^CGAT
DrdI 262 GACTATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2941 TGGCCA [CT]^GGCC[GA]
Eco47III 5206 AGCGCT AGC^GCT
EcoNI 4768 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3776 GGTGACC G^GT[ACGT]ACC
EcoRV 4349 GATATC GAT^ATC
FauI 127 CCCGC CCCGC(4/6)
FbaI 4068 TGATCA T^GATCA
HpaI 2327 GTTAAC GTT^AAC
KpnI 1040 GGTACC GGTAC^C
MluNI 2941 TGGCCA TGG^CCA
MroI 3387 TCCGGA T^CCGGA
MscI 2941 TGGCCA TGG^CCA
NcoI 171 CCATGG C^CATGG
PaeR7I 3663 CTCGAG C^TCGAG
PflFI 2516 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3285 CACGTG CAC^GTG
PmlI 3285 CACGTG CAC^GTG
PshBI 2963 ATTAAT AT^TAAT
SalI 2601 GTCGAC G^TCGAC
SmaI 111 CCCGGG CCC^GGG
SpeI 3608 ACTAGT A^CTAGT
SphI 1942 GCATGC GCATG^C
StuI 1481 AGGCCT AGG^CCT
SwaI 5605 ATTTAAAT ATTT^AAAT
Tth111I 2516 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4193 TCTAGA T^CTAGA
XhoI 3663 CTCGAG C^TCGAG
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1917 CGCG CG^CG
2879 CGCG
AclI 811 AACGTT AA^CGTT
3471 AACGTT
AocI 1609 CCTAAGG CC^T[ACGT]AGG
5966 CCTGAGG
BlpI 63 GCTGAGC GC^T[ACGT]AGC
4658 GCTCAGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
4658 GCTCAGC
BsaBI 2909 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5933 GATTCCAATC
BstBI 357 TTCGAA TT^CGAA
5402 TTCGAA
BstUI 1917 CGCG CG^CG
2879 CGCG
BstXI 3029 CCAGAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3766 CCAACAAGTTGG
Bsu36I 1609 CCTAAGG CC^T[ACGT]AGG
5966 CCTGAGG
CelII 63 GCTGAGC GC^T[ACGT]AGC
4658 GCTCAGC
DraIII 1596 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
6006 CACTGAGTG
DsaI 171 CCATGG C^C[GA][CT]GG
4454 CCACGG
Eco81I 1609 CCTAAGG CC^T[ACGT]AGG
5966 CCTGAGG
HgaI 1915 GACGC GACGC(5/10)
43 GCGTC
MamI 2909 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5933 GATTCCAATC
MspA1I 1107 CAGCTG C[AC]G^C[GT]G
3888 CAGCTG
MvnI 1917 CGCG CG^CG
2879 CGCG
NheI 398 GCTAGC G^CTAGC
767 GCTAGC
NspV 357 TTCGAA TT^CGAA
5402 TTCGAA
Psp1406I 811 AACGTT AA^CGTT
3471 AACGTT
PvuII 1107 CAGCTG CAG^CTG
3888 CAGCTG
SacI 14 GAGCTC GAGCT^C
4104 GAGCTC
SapI 1790 GAAGAGC GCTCTTC(1/4)
4061 GAAGAGC
SexAI 3009 ACCTGGT A^CC[AT]GGT
3087 ACCAGGT
SfuI 357 TTCGAA TT^CGAA
5402 TTCGAA
SnaBI 1230 TACGTA TAC^GTA
5587 TACGTA
XcmI 4316 CCAACTGATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5125 CCAGGCACCTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI