##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccIII AcyI AgeI
AhdI AlwI Aor51HI ApaI AscI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbvCI BcgI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII Eam1105I Eco47III EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KspI MamI MflI MluI
MroI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PshAI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I Van91I XbaI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 3119 AACGTT AA^CGTT
AflII 3544 CTTAAG C^TTAAG
Alw44I 2575 GTGCAC G^TGCAC
ApaLI 2575 GTGCAC G^TGCAC
AseI 3063 ATTAAT AT^TAAT
AsnI 3063 ATTAAT AT^TAAT
AvaI 604 CTCGAG C^[CT]CG[GA]G
BbrPI 2531 CACGTG CAC^GTG
BciVI 38 GTATCC GTATCC(6/5)
BclI 3090 TGATCA T^GATCA
BfrI 3544 CTTAAG C^TTAAG
BsaI 2203 GGTCTC GGTCTC(1/5)
BsiEI 1 CGGCCG CG[GA][CT]^CG
BsoBI 604 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2303 TGTACA T^GTACA
BspLU11I 2579 ACATGT A^CATGT
BsrDI 2650 GCAATG GCAATG(2/0)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
BsrGI 2303 TGTACA T^GTACA
CfoI 62 GCGC GCG^C
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
DrdI 1478 GACATCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2795 CCATGG C^C[GA][CT]GG
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco52I 1 CGGCCG C^GGCCG
EcoRI 1181 GAATTC G^AATTC
FbaI 3090 TGATCA T^GATCA
HhaI 62 GCGC GCG^C
HinP1I 62 GCGC G^CGC
MfeI 3299 CAATTG C^AATTG
MslI 3492 CATTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3299 CAATTG C^AATTG
NaeI 6 GCCGGC GCC^GGC
NcoI 2795 CCATGG C^CATGG
NgoMIV 6 GCCGGC G^CCGGC
PaeR7I 604 CTCGAG C^TCGAG
PmaCI 2531 CACGTG CAC^GTG
PmlI 2531 CACGTG CAC^GTG
PshBI 3063 ATTAAT AT^TAAT
Psp1406I 3119 AACGTT AA^CGTT
SapI 1108 GCTCTTC GCTCTTC(1/4)
SfiI 3385 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2664 GCATGC GCATG^C
SspBI 2303 TGTACA T^GTACA
StuI 238 AGGCCT AGG^CCT
TaqI 605 TCGA T^CGA
TthHB8I 605 TCGA T^CGA
XhoI 604 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1170 GGTACC G^GTACC
3287 GGTACC
AccII 18 CGCG CG^CG
63 CGCG
AflIII 2532 ACGTGT A^C[GA][CT]GT
2579 ACATGT
Asp718I 1170 GGTACC G^GTACC
3287 GGTACC
BcnI 808 CCCGG CC^[GC]GG
2072 CCGGG
BsaAI 2531 CACGTG [CT]AC^GT[GA]
2854 TACGTG
BspMI 317 ACCTGC ACCTGC(4/8)
1217 ACCTGC
BssSI 1936 CACGAG CACGAG(-5/-1)
45 CTCGTG
BstUI 18 CGCG CG^CG
63 CGCG
DpnII 2159 GATC ^GATC
3091 GATC
FauI 905 CCCGC CCCGC(4/6)
2060 GCGGG
HindIII 1269 AAGCTT A^AGCTT
1836 AAGCTT
KpnI 1170 GGTACC GGTAC^C
3287 GGTACC
MboI 2159 GATC ^GATC
3091 GATC
MvnI 18 CGCG CG^CG
63 CGCG
NciI 808 CCCGG CC^[GC]GG
2072 CCGGG
NdeII 2159 GATC ^GATC
3091 GATC
SacI 491 GAGCTC GAGCT^C
1106 GAGCTC
Sau3AI 2159 GATC ^GATC
3091 GATC
SexAI 881 ACCAGGT A^CC[AT]GGT
3600 ACCTGGT
XcmI 952 CCAAGAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1243 CCAAGCAACCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
AocI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI